Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes Ecosystem
The coastal sand dune ecosystem at the Parangtritis Coast of Yogyakarta, Indonesia has unique characteristics such as low moisture sandy soil, high salinity and low nutrient content. Fimbristylis cymosa is one of the plant species having the capability to survive in that unique ecosystem. In this s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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SEAMEO, BIOTROP
2022-04-01
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| Series: | Biotropia: The Southeast Asian Journal of Tropical Biology |
| Online Access: | https://journal.biotrop.org/index.php/biotropia/article/view/1584 |
| _version_ | 1827902857212329984 |
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| author | Ketut Arte Widane Annisaa Widyasari Endah Retnaningrum |
| author_facet | Ketut Arte Widane Annisaa Widyasari Endah Retnaningrum |
| author_sort | Ketut Arte Widane |
| collection | DOAJ |
| description |
The coastal sand dune ecosystem at the Parangtritis Coast of Yogyakarta, Indonesia has unique characteristics such as low moisture sandy soil, high salinity and low nutrient content. Fimbristylis cymosa is one of the plant species having the capability to survive in that unique ecosystem. In this study, rhizobacteria isolated from the rhizosphere of F. cymosa were isolated to be further analyzed on their phosphate solubilizing and antagonistic properties against Fusarium oxysporum which cause the Wilt disease. The isolates of Phosphate Solubilizing Rhizobacteria (PSR) having the most potential capabilities were then polyphasically identified based on phenotypic and genotypic characters followed by 16S rDNA sequencing. The results showed that four PSR isolates (I8, I11, I12 and I24) have high phosphate dissolution indices. The highest indices were observed in isolates I11 (3.08) and I12 (3.44), respectively. Analysis of the dual plate experiments for PSR I11 and PSR I12 isolates against the growth of F. oxysporum also showed quite high inhibitory activities, i.e., isolate PSR I11 was 42.40%, while isolate PSR I12 was 42.08%. The two isolates were polyphasically identified as Burkholderia dolosa. This study clearly showed that PSR I11 and PSR I12 isolates are very potential and prospective to be used as marginal land inoculants and as providers of phosphorus. This study also showed that the isolates are useful as biocontrol agents against F. oxysporum in plants.
Keywords: inhibitory activity, phosphate dissolution index, phosphorus, polyphasic identification, sandy soil
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| first_indexed | 2024-03-13T00:05:04Z |
| format | Article |
| id | doaj.art-a635949994804f49a3890b3cd7f7306e |
| institution | Directory Open Access Journal |
| issn | 0215-6334 1907-770X |
| language | English |
| last_indexed | 2024-03-13T00:05:04Z |
| publishDate | 2022-04-01 |
| publisher | SEAMEO, BIOTROP |
| record_format | Article |
| series | Biotropia: The Southeast Asian Journal of Tropical Biology |
| spelling | doaj.art-a635949994804f49a3890b3cd7f7306e2023-07-13T04:23:47ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2022-04-0129110.11598/btb.2022.29.1.1584Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes EcosystemKetut Arte Widane0Annisaa Widyasari1Endah Retnaningrum2Undergraduate Student, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia Graduate Student, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaMicrobiology Laboratory, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia The coastal sand dune ecosystem at the Parangtritis Coast of Yogyakarta, Indonesia has unique characteristics such as low moisture sandy soil, high salinity and low nutrient content. Fimbristylis cymosa is one of the plant species having the capability to survive in that unique ecosystem. In this study, rhizobacteria isolated from the rhizosphere of F. cymosa were isolated to be further analyzed on their phosphate solubilizing and antagonistic properties against Fusarium oxysporum which cause the Wilt disease. The isolates of Phosphate Solubilizing Rhizobacteria (PSR) having the most potential capabilities were then polyphasically identified based on phenotypic and genotypic characters followed by 16S rDNA sequencing. The results showed that four PSR isolates (I8, I11, I12 and I24) have high phosphate dissolution indices. The highest indices were observed in isolates I11 (3.08) and I12 (3.44), respectively. Analysis of the dual plate experiments for PSR I11 and PSR I12 isolates against the growth of F. oxysporum also showed quite high inhibitory activities, i.e., isolate PSR I11 was 42.40%, while isolate PSR I12 was 42.08%. The two isolates were polyphasically identified as Burkholderia dolosa. This study clearly showed that PSR I11 and PSR I12 isolates are very potential and prospective to be used as marginal land inoculants and as providers of phosphorus. This study also showed that the isolates are useful as biocontrol agents against F. oxysporum in plants. Keywords: inhibitory activity, phosphate dissolution index, phosphorus, polyphasic identification, sandy soil https://journal.biotrop.org/index.php/biotropia/article/view/1584 |
| spellingShingle | Ketut Arte Widane Annisaa Widyasari Endah Retnaningrum Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes Ecosystem Biotropia: The Southeast Asian Journal of Tropical Biology |
| title | Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes Ecosystem |
| title_full | Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes Ecosystem |
| title_fullStr | Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes Ecosystem |
| title_full_unstemmed | Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes Ecosystem |
| title_short | Characterization and Polyphasic Identification of Novel Rhizobacteria Strain Isolated from Sand Dunes Ecosystem |
| title_sort | characterization and polyphasic identification of novel rhizobacteria strain isolated from sand dunes ecosystem |
| url | https://journal.biotrop.org/index.php/biotropia/article/view/1584 |
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