Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.)
The SQUAMOSA PROMOTER BINDING PROTEIN (SBP) gene family is essential for plant growth and development. However, little is known about the SBP gene family in pitaya (Selenicereus undatus L.); a fruit crop cultivated in tropical climates, severely affected by abiotic stress factors. These stresses pro...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Plant Stress |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23001616 |
| _version_ | 1797422518688022528 |
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| author | Aamir Ali Khokhar Liu Hui Darya Khan Muhammad Azhar Hussain Wei Lv Qamar U Zaman Hua-Feng Wang |
| author_facet | Aamir Ali Khokhar Liu Hui Darya Khan Muhammad Azhar Hussain Wei Lv Qamar U Zaman Hua-Feng Wang |
| author_sort | Aamir Ali Khokhar |
| collection | DOAJ |
| description | The SQUAMOSA PROMOTER BINDING PROTEIN (SBP) gene family is essential for plant growth and development. However, little is known about the SBP gene family in pitaya (Selenicereus undatus L.); a fruit crop cultivated in tropical climates, severely affected by abiotic stress factors. These stresses produce excessive reactive oxygen species (ROS), affecting pitaya growth and development. In this study, we identified 17 SBP genes in the pitaya genome (HuSBP) and classified them into seven distinct clusters based on their phylogeny. The HuSBP genes exhibited a conserved intron-exon structure and motif domain organization. These SBP genes were differentially expressed in 15 developmental stages of flower (F), flower buds (FB), pericarp (PeriC), and pulp. In addition, specific HuSBP genes exhibited higher expression under single-factor, double-factor, and multifactorial stresses, revealing their diverse role in the growth and development of pitaya. Exogenous application of melatonin with known antioxidant and stress-protective properties, was also applied. The expression pattern of RNA-Seq data was validated by RT-qPCR analysis. Based on genome-wide results and transcriptome-based data under multifactorial stresses, we predicted six putative candidate genes (HuSBP2/3/7/10/14/15) that might play diverse roles in pitaya growth and development. These results provide valuable insights into the role of HuSBP genes in pitaya stress tolerance and could be used to develop innovative strategies to create resilient and adaptable pitaya genotypes using conventional breeding and modern biotechnological tools. |
| first_indexed | 2024-03-09T07:33:27Z |
| format | Article |
| id | doaj.art-a636160d3a494d008fde9bd725a84124 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-03-09T07:33:27Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-a636160d3a494d008fde9bd725a841242023-12-03T05:43:41ZengElsevierPlant Stress2667-064X2023-12-0110100294Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.)Aamir Ali Khokhar0Liu Hui1Darya Khan2Muhammad Azhar Hussain3Wei Lv4Qamar U Zaman5Hua-Feng Wang6School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, ChinaSchool of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China; Corresponding author.School of Breeding and Multiplication (Sanya Institute of Breeding and Multiplication), Hainan Yazhou Bay Seed Laboratory, Hainan University, Sanya 572025, China; School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China; Corresponding author.The SQUAMOSA PROMOTER BINDING PROTEIN (SBP) gene family is essential for plant growth and development. However, little is known about the SBP gene family in pitaya (Selenicereus undatus L.); a fruit crop cultivated in tropical climates, severely affected by abiotic stress factors. These stresses produce excessive reactive oxygen species (ROS), affecting pitaya growth and development. In this study, we identified 17 SBP genes in the pitaya genome (HuSBP) and classified them into seven distinct clusters based on their phylogeny. The HuSBP genes exhibited a conserved intron-exon structure and motif domain organization. These SBP genes were differentially expressed in 15 developmental stages of flower (F), flower buds (FB), pericarp (PeriC), and pulp. In addition, specific HuSBP genes exhibited higher expression under single-factor, double-factor, and multifactorial stresses, revealing their diverse role in the growth and development of pitaya. Exogenous application of melatonin with known antioxidant and stress-protective properties, was also applied. The expression pattern of RNA-Seq data was validated by RT-qPCR analysis. Based on genome-wide results and transcriptome-based data under multifactorial stresses, we predicted six putative candidate genes (HuSBP2/3/7/10/14/15) that might play diverse roles in pitaya growth and development. These results provide valuable insights into the role of HuSBP genes in pitaya stress tolerance and could be used to develop innovative strategies to create resilient and adaptable pitaya genotypes using conventional breeding and modern biotechnological tools.http://www.sciencedirect.com/science/article/pii/S2667064X23001616Dragon fruit (Selenicereus undatus L.)SBP gene familyAbiotic stress combinationsTranscriptome analysisPitayaMelatonin |
| spellingShingle | Aamir Ali Khokhar Liu Hui Darya Khan Muhammad Azhar Hussain Wei Lv Qamar U Zaman Hua-Feng Wang Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.) Plant Stress Dragon fruit (Selenicereus undatus L.) SBP gene family Abiotic stress combinations Transcriptome analysis Pitaya Melatonin |
| title | Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.) |
| title_full | Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.) |
| title_fullStr | Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.) |
| title_full_unstemmed | Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.) |
| title_short | Comprehensive characterization of SBP genes revealed their role under multifactorial stress combinations in dragon fruit (Selenicereus undatus L.) |
| title_sort | comprehensive characterization of sbp genes revealed their role under multifactorial stress combinations in dragon fruit selenicereus undatus l |
| topic | Dragon fruit (Selenicereus undatus L.) SBP gene family Abiotic stress combinations Transcriptome analysis Pitaya Melatonin |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23001616 |
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