Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma
Background: Recent findings indicate that the host microbiome can have a significant impact on the development of lung cancer by inducing an inflammatory response, causing dysbiosis, and generating genome damage. The aim of this study was to search for bacterial communities specifically associated w...
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MDPI AG
2022-09-01
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| Online Access: | https://www.mdpi.com/2075-1729/12/9/1365 |
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| author | Elizaveta Baranova Vladimir Druzhinin Ludmila Matskova Pavel Demenkov Valentin Volobaev Varvara Minina Alexey Larionov Victor Titov |
| author_facet | Elizaveta Baranova Vladimir Druzhinin Ludmila Matskova Pavel Demenkov Valentin Volobaev Varvara Minina Alexey Larionov Victor Titov |
| author_sort | Elizaveta Baranova |
| collection | DOAJ |
| description | Background: Recent findings indicate that the host microbiome can have a significant impact on the development of lung cancer by inducing an inflammatory response, causing dysbiosis, and generating genome damage. The aim of this study was to search for bacterial communities specifically associated with squamous cell carcinoma (LUSC). Methods: In this study, the taxonomic composition of the sputum microbiome of 40 men with untreated LUSC was compared with that of 40 healthy controls. Next-Generation sequencing of bacterial 16S rRNA genes was used to determine the taxonomic composition of the respiratory microbiome. Results: There were no differences in alpha diversity between the LUSC and control groups. Meanwhile, differences in the structure of bacterial communities (β diversity) among patients and controls differed significantly in sputum samples (pseudo-F = 1.53; <i>p</i> = 0.005). Genera of <i>Streptococcus</i>, <i>Bacillus</i>, <i>Gemella,</i> and <i>Haemophilus</i> were found to be significantly enriched in patients with LUSC compared to the control subjects, while 19 bacterial genera were significantly reduced, indicating a decrease in beta diversity in the microbiome of patients with LUSC. Conclusions: Among other candidates, <i>Streptococcus</i> (<i>Streptococcus agalactiae</i>) emerges as the most likely LUSC biomarker, but more research is needed to confirm this assumption. |
| first_indexed | 2024-03-09T23:23:38Z |
| format | Article |
| id | doaj.art-a6369ef64b2a46939dca990e0ccfc4ea |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T23:23:38Z |
| publishDate | 2022-09-01 |
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| record_format | Article |
| series | Life |
| spelling | doaj.art-a6369ef64b2a46939dca990e0ccfc4ea2023-11-23T17:22:52ZengMDPI AGLife2075-17292022-09-01129136510.3390/life12091365Sputum Microbiome Composition in Patients with Squamous Cell Lung CarcinomaElizaveta Baranova0Vladimir Druzhinin1Ludmila Matskova2Pavel Demenkov3Valentin Volobaev4Varvara Minina5Alexey Larionov6Victor Titov7Department of Genetics and Fundamental Medicine, Kemerovo State University, Kemerovo 650000, RussiaDepartment of Genetics and Fundamental Medicine, Kemerovo State University, Kemerovo 650000, RussiaInstitute of Living Systems, Immanuel Kant Baltic Federal University, Kaliningrad 236041, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk 630090, RussiaScientific Center for Genetics and Life Sciences, Sirius University of Science and Technology, Sochi 354340, RussiaDepartment of Genetics and Fundamental Medicine, Kemerovo State University, Kemerovo 650000, RussiaDepartment of Genetics and Fundamental Medicine, Kemerovo State University, Kemerovo 650000, RussiaKemerovo Regional Oncology Center, Kemerovo 654005, RussiaBackground: Recent findings indicate that the host microbiome can have a significant impact on the development of lung cancer by inducing an inflammatory response, causing dysbiosis, and generating genome damage. The aim of this study was to search for bacterial communities specifically associated with squamous cell carcinoma (LUSC). Methods: In this study, the taxonomic composition of the sputum microbiome of 40 men with untreated LUSC was compared with that of 40 healthy controls. Next-Generation sequencing of bacterial 16S rRNA genes was used to determine the taxonomic composition of the respiratory microbiome. Results: There were no differences in alpha diversity between the LUSC and control groups. Meanwhile, differences in the structure of bacterial communities (β diversity) among patients and controls differed significantly in sputum samples (pseudo-F = 1.53; <i>p</i> = 0.005). Genera of <i>Streptococcus</i>, <i>Bacillus</i>, <i>Gemella,</i> and <i>Haemophilus</i> were found to be significantly enriched in patients with LUSC compared to the control subjects, while 19 bacterial genera were significantly reduced, indicating a decrease in beta diversity in the microbiome of patients with LUSC. Conclusions: Among other candidates, <i>Streptococcus</i> (<i>Streptococcus agalactiae</i>) emerges as the most likely LUSC biomarker, but more research is needed to confirm this assumption.https://www.mdpi.com/2075-1729/12/9/1365lung cancersquamous cell lung carcinomasputum microbiometaxonomic composition<i>Streptococcus</i> |
| spellingShingle | Elizaveta Baranova Vladimir Druzhinin Ludmila Matskova Pavel Demenkov Valentin Volobaev Varvara Minina Alexey Larionov Victor Titov Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma Life lung cancer squamous cell lung carcinoma sputum microbiome taxonomic composition <i>Streptococcus</i> |
| title | Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma |
| title_full | Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma |
| title_fullStr | Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma |
| title_full_unstemmed | Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma |
| title_short | Sputum Microbiome Composition in Patients with Squamous Cell Lung Carcinoma |
| title_sort | sputum microbiome composition in patients with squamous cell lung carcinoma |
| topic | lung cancer squamous cell lung carcinoma sputum microbiome taxonomic composition <i>Streptococcus</i> |
| url | https://www.mdpi.com/2075-1729/12/9/1365 |
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