High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff)
Abstract Background The brown planthopper (Nilaparvata lugens Stål; BPH), one of the most destructive pests of rice, has proven to be a substantial threat, conferring enormous production losses in Asia and becoming a difficult challenge to manipulate and control under field conditions. The continuou...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2019-06-01
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| Series: | Rice |
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| Online Access: | http://link.springer.com/article/10.1186/s12284-019-0289-7 |
| _version_ | 1818510452612661248 |
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| author | Zhihua Li Yanxia Xue Hailian Zhou Yang Li Babar Usman Xiaozhen Jiao Xinyi Wang Fang Liu Baoxiang Qin Rongbai Li Yongfu Qiu |
| author_facet | Zhihua Li Yanxia Xue Hailian Zhou Yang Li Babar Usman Xiaozhen Jiao Xinyi Wang Fang Liu Baoxiang Qin Rongbai Li Yongfu Qiu |
| author_sort | Zhihua Li |
| collection | DOAJ |
| description | Abstract Background The brown planthopper (Nilaparvata lugens Stål; BPH), one of the most destructive pests of rice, has proven to be a substantial threat, conferring enormous production losses in Asia and becoming a difficult challenge to manipulate and control under field conditions. The continuous use of insecticides promotes the resurgence of BPH, which results in resistant varieties adapting through the upgrading of new BPH biotypes. To overcome resistance acquired by BPH against resistance varieties, different forms of novel resistant gene fusions act as functional domains for breeding to enhance insect resistance. Results The current study reports on the novel BPH resistance gene Bph36 derived from two introgression lines (RBPH16 and RBPH17) developed from wild rice GX2183 which was previously reported to be resistant to BPH. Using two F2 crossing populations (Kangwenqizhan × RBPH16 and Huanghuazhan × RBPH17) in a bulked segregant analysis (BSA) for identification of resistant genes and QTL analysis, two QTLs for BPH resistance were generated on the long and short arms of chromosome 4, which was further confirmed by developing BC1F2:3 populations by backcrossing via marker assisted selection (MAS) approach. One BPH resistance locus on the short arm of chromosome 4 was mapped to a 38-kb interval flanked by InDel markers S13 and X48, and then was named Bph36, whereas another locus on the long arm of chromosome 4 was also detected in an interval flanked by RM16766 and RM17033, which was the same as that of Bph27. An evaluation analysis based on four parameters (BPH host selection, honeydew weight, BPH survival rate and BPH population growth rate) shows that Bph36 conferred high levels antibiosis and antixenosis to BPH. Moreover, Bph36 pyramided with Bph3, Bph27, and Bph29 through MAS into elite cultivars 9311 and MH511 (harbored Xa23), creating different background breeding lines that also exhibited strong resistance to BPH in the seedling or tillering stage. Conclusion Bph36 can be utilized in BPH resistance breeding programs to develop high resistant rice lines and the high-resolution fine mapping will facilitate further map-based cloning and marker-assisted gene pyramiding of resistant gene. MAS exploited to pyramid with Bph3, Bph27, Bph29, and Xa23 was confirmed the effectiveness for BPH resistance breeding in rice and provided insights into the molecular mechanism of defense to control this devastating insect. |
| first_indexed | 2024-12-10T23:20:08Z |
| format | Article |
| id | doaj.art-a638dc2de43d4b52b90ef44e2311be26 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-12-10T23:20:08Z |
| publishDate | 2019-06-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-a638dc2de43d4b52b90ef44e2311be262022-12-22T01:29:45ZengSpringerOpenRice1939-84251939-84332019-06-0112111310.1186/s12284-019-0289-7High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff)Zhihua Li0Yanxia Xue1Hailian Zhou2Yang Li3Babar Usman4Xiaozhen Jiao5Xinyi Wang6Fang Liu7Baoxiang Qin8Rongbai Li9Yongfu Qiu10State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Agricultural College, Guangxi UniversityAbstract Background The brown planthopper (Nilaparvata lugens Stål; BPH), one of the most destructive pests of rice, has proven to be a substantial threat, conferring enormous production losses in Asia and becoming a difficult challenge to manipulate and control under field conditions. The continuous use of insecticides promotes the resurgence of BPH, which results in resistant varieties adapting through the upgrading of new BPH biotypes. To overcome resistance acquired by BPH against resistance varieties, different forms of novel resistant gene fusions act as functional domains for breeding to enhance insect resistance. Results The current study reports on the novel BPH resistance gene Bph36 derived from two introgression lines (RBPH16 and RBPH17) developed from wild rice GX2183 which was previously reported to be resistant to BPH. Using two F2 crossing populations (Kangwenqizhan × RBPH16 and Huanghuazhan × RBPH17) in a bulked segregant analysis (BSA) for identification of resistant genes and QTL analysis, two QTLs for BPH resistance were generated on the long and short arms of chromosome 4, which was further confirmed by developing BC1F2:3 populations by backcrossing via marker assisted selection (MAS) approach. One BPH resistance locus on the short arm of chromosome 4 was mapped to a 38-kb interval flanked by InDel markers S13 and X48, and then was named Bph36, whereas another locus on the long arm of chromosome 4 was also detected in an interval flanked by RM16766 and RM17033, which was the same as that of Bph27. An evaluation analysis based on four parameters (BPH host selection, honeydew weight, BPH survival rate and BPH population growth rate) shows that Bph36 conferred high levels antibiosis and antixenosis to BPH. Moreover, Bph36 pyramided with Bph3, Bph27, and Bph29 through MAS into elite cultivars 9311 and MH511 (harbored Xa23), creating different background breeding lines that also exhibited strong resistance to BPH in the seedling or tillering stage. Conclusion Bph36 can be utilized in BPH resistance breeding programs to develop high resistant rice lines and the high-resolution fine mapping will facilitate further map-based cloning and marker-assisted gene pyramiding of resistant gene. MAS exploited to pyramid with Bph3, Bph27, Bph29, and Xa23 was confirmed the effectiveness for BPH resistance breeding in rice and provided insights into the molecular mechanism of defense to control this devastating insect.http://link.springer.com/article/10.1186/s12284-019-0289-7Oryza rufipogon GriffBrown planthopper (Nilaparvata lugens Stål)Resistance geneGene mappingMarker-assisted selectionNear isogenic line |
| spellingShingle | Zhihua Li Yanxia Xue Hailian Zhou Yang Li Babar Usman Xiaozhen Jiao Xinyi Wang Fang Liu Baoxiang Qin Rongbai Li Yongfu Qiu High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff) Rice Oryza rufipogon Griff Brown planthopper (Nilaparvata lugens Stål) Resistance gene Gene mapping Marker-assisted selection Near isogenic line |
| title | High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff) |
| title_full | High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff) |
| title_fullStr | High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff) |
| title_full_unstemmed | High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff) |
| title_short | High-resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice (Oryza. rufipogon Griff) |
| title_sort | high resolution mapping and breeding application of a novel brown planthopper resistance gene derived from wild rice oryza rufipogon griff |
| topic | Oryza rufipogon Griff Brown planthopper (Nilaparvata lugens Stål) Resistance gene Gene mapping Marker-assisted selection Near isogenic line |
| url | http://link.springer.com/article/10.1186/s12284-019-0289-7 |
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