Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes
Abstract The study investigated the effect of enzymes as a toxin detoxifier (DETOXIZYME) dietary supplementation on performance during growth, blood chemistry, and immunity under clostridia infection in chickens. A total of 480, day-old male chicks were randomly distributed to four groups, with six...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-32685-3 |
| _version_ | 1797850116218945536 |
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| author | Ahmed A. Saleh Abdelhaleem Hafez Khairy Amber AbdelRahman Y. Abdelhady Heba M. Salem M. Fathy M. A. Kamal Mahmoud Alagawany Mohammed H. Alzawqari |
| author_facet | Ahmed A. Saleh Abdelhaleem Hafez Khairy Amber AbdelRahman Y. Abdelhady Heba M. Salem M. Fathy M. A. Kamal Mahmoud Alagawany Mohammed H. Alzawqari |
| author_sort | Ahmed A. Saleh |
| collection | DOAJ |
| description | Abstract The study investigated the effect of enzymes as a toxin detoxifier (DETOXIZYME) dietary supplementation on performance during growth, blood chemistry, and immunity under clostridia infection in chickens. A total of 480, day-old male chicks were randomly distributed to four groups, with six replicates of 20 birds each. The first control negative treatment (A) fed the basal formula as commercial feed prepared following the strain's needs, the second control positive group (B) fed the basal formula challenged with Clostridium perfringens (C. perfringens) type A, the third group (C) fed the basal formula with 100 g DETOXIZYME/ton of feed and challenged with clostridia, and the fourth group (D) fed the control basal formula with 100 g DETOXIZYME/ton of feed. DETOXIZYME dietary supplementation significantly boosted body weight (BW), body weight gain (BWG), feed intake (FI), and European production efficiency factor (EPEF) and improved the feed conversion rate (FCR) of the broilers. The dietary supplementation of DETOXIZYME significantly increased carcass trait and spleen. However, liver and abdominal fat weight significantly decreased compared with clostridia-challenged groups. The values of alanine aminotransferase (ALT), aspartate aminotransferase (AST), uric acid, creatinine, and Malondialdehyde (MDA) were decreased. While calcium, phosphate, zinc, and glutathione peroxidase (GPx) levels were improved in birds that took basal formulas fortified with DETOXIZYME contrary to the other treatment groups during 35 days of age. Plasma total cholesterol, triglyceride, and low-density lipoprotein (LDL) values were reduced versus the other treatment groups. Dietary supplementation of DETOXIZYME increased total protein, albumin, globulin, and Newcastle Disease (ND) immunity titer levels in the overall period compared to other groups. Dietary DETOXIZYME supplementation decreased clostridia and E. coli bacteria counts and improved gut morphometry. In conclusion, dietary supplementation of DETOXIZYME had a positive impact on performance, blood biochemistry, immunity, and bacterial counts and improved the gut morphology in broilers under clostridia infection. |
| first_indexed | 2024-04-09T18:56:15Z |
| format | Article |
| id | doaj.art-a63d8f905b76495181efb7b1b235927e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T18:56:15Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a63d8f905b76495181efb7b1b235927e2023-04-09T11:12:15ZengNature PortfolioScientific Reports2045-23222023-04-0113111310.1038/s41598-023-32685-3Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymesAhmed A. Saleh0Abdelhaleem Hafez1Khairy Amber2AbdelRahman Y. Abdelhady3Heba M. Salem4M. Fathy5M. A. Kamal6Mahmoud Alagawany7Mohammed H. Alzawqari8Department of Poultry Production, Faculty of Agriculture, Kafrelsheikh UniversityDepartment of Poultry Production, Faculty of Agriculture, Kafrelsheikh UniversityDepartment of Poultry Production, Faculty of Agriculture, Kafrelsheikh UniversityPoultry Production Department, Faculty of Agriculture, Ain Shams UniversityDepartment of Poultry Diseases, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Animal and Poultry Health, Desert Research CentreDepartment of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo UniversityDepartment of Poultry, Faculty of Agriculture, Zagazig UniversityDepartment of Animal Production, Faculty of Agriculture and Food Sciences, Ibb UniversityAbstract The study investigated the effect of enzymes as a toxin detoxifier (DETOXIZYME) dietary supplementation on performance during growth, blood chemistry, and immunity under clostridia infection in chickens. A total of 480, day-old male chicks were randomly distributed to four groups, with six replicates of 20 birds each. The first control negative treatment (A) fed the basal formula as commercial feed prepared following the strain's needs, the second control positive group (B) fed the basal formula challenged with Clostridium perfringens (C. perfringens) type A, the third group (C) fed the basal formula with 100 g DETOXIZYME/ton of feed and challenged with clostridia, and the fourth group (D) fed the control basal formula with 100 g DETOXIZYME/ton of feed. DETOXIZYME dietary supplementation significantly boosted body weight (BW), body weight gain (BWG), feed intake (FI), and European production efficiency factor (EPEF) and improved the feed conversion rate (FCR) of the broilers. The dietary supplementation of DETOXIZYME significantly increased carcass trait and spleen. However, liver and abdominal fat weight significantly decreased compared with clostridia-challenged groups. The values of alanine aminotransferase (ALT), aspartate aminotransferase (AST), uric acid, creatinine, and Malondialdehyde (MDA) were decreased. While calcium, phosphate, zinc, and glutathione peroxidase (GPx) levels were improved in birds that took basal formulas fortified with DETOXIZYME contrary to the other treatment groups during 35 days of age. Plasma total cholesterol, triglyceride, and low-density lipoprotein (LDL) values were reduced versus the other treatment groups. Dietary supplementation of DETOXIZYME increased total protein, albumin, globulin, and Newcastle Disease (ND) immunity titer levels in the overall period compared to other groups. Dietary DETOXIZYME supplementation decreased clostridia and E. coli bacteria counts and improved gut morphometry. In conclusion, dietary supplementation of DETOXIZYME had a positive impact on performance, blood biochemistry, immunity, and bacterial counts and improved the gut morphology in broilers under clostridia infection.https://doi.org/10.1038/s41598-023-32685-3 |
| spellingShingle | Ahmed A. Saleh Abdelhaleem Hafez Khairy Amber AbdelRahman Y. Abdelhady Heba M. Salem M. Fathy M. A. Kamal Mahmoud Alagawany Mohammed H. Alzawqari Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes Scientific Reports |
| title | Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes |
| title_full | Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes |
| title_fullStr | Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes |
| title_full_unstemmed | Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes |
| title_short | Drug-independent control strategy of clostridial infection in broiler chickens using anti-toxin environmentally friendly multienzymes |
| title_sort | drug independent control strategy of clostridial infection in broiler chickens using anti toxin environmentally friendly multienzymes |
| url | https://doi.org/10.1038/s41598-023-32685-3 |
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