The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa
Abstract Objectives Staphylococcus aureus and Pseudomonas aeruginosa were the most common bacteria in nosocomial infections. Different bacteriocins are currently being studied as antibiotics or in conjunction with antibiotics as potential strategies to treat resistant infectious agents. The study ai...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-022-00804-x |
| _version_ | 1797836565006778368 |
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| author | Parnia Ghapanvari Mohammad Taheri Farid Aziz Jalilian Sanaz Dehbashi Aram Asareh Zadegan Dezfuli Mohammad Reza Arabestani |
| author_facet | Parnia Ghapanvari Mohammad Taheri Farid Aziz Jalilian Sanaz Dehbashi Aram Asareh Zadegan Dezfuli Mohammad Reza Arabestani |
| author_sort | Parnia Ghapanvari |
| collection | DOAJ |
| description | Abstract Objectives Staphylococcus aureus and Pseudomonas aeruginosa were the most common bacteria in nosocomial infections. Different bacteriocins are currently being studied as antibiotics or in conjunction with antibiotics as potential strategies to treat resistant infectious agents. The study aimed to determine nisin's effect on the biofilm production, antimicrobial susceptibility, and biofilm formation of S. aureus and P. aeruginosa. Materials and methods The experimental research tested two antibiotic-resistant isolates of S. aureus and P. aeruginosa strains. The experimental study tested two antibiotic-resistant isolates of S. aureus and P. aeruginosa strains. The MIC of bacteriocin nisin was determined using the micro broth dilution method, and crystal violet was used to assess the effect of bacteriocin on the biofilm. In addition, L929 cell culture was used to determine the effectiveness of bacteriocin on the isolate under similar cell conditions. Moreover, the MTT assay was used to and evaluate bacteriocin toxicity. In this study, the software Prism version 9 and Graph pad software were utilized. Results The results of this study reveal that the nisin has different activities at different doses and is considered dose-dependent. At various times and doses, nisin inhibits biofilm formation in S. aureus, and P. aeruginosa isolates. Nisin also showed a decreasing survival of the isolates. Antibiotic-resistant bacteria can be made more vulnerable by nisin. Furthermore, nisin treatment affected the production of virulence factors such as hemolysins in S. aureus and had little or a negative effect on P. aeruginosa virulence factors. This medication stops S. aureus and P. aeruginosa from growing and causes bacterial cell damage. Conclusions Antibacterial properties of nicin against S. aureus and P. aeruginosa were successfully studied. This bacteriocin stops S. aureus and P. aeruginosa from growing and causes bacterial cell damage or death. Damage to the membrane among the fundamental causes is reduced membrane potential and enzyme inactivation. |
| first_indexed | 2024-04-09T15:12:05Z |
| format | Article |
| id | doaj.art-a6404c1dc0bf4fbfabc692e81fb6ff89 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-04-09T15:12:05Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-a6404c1dc0bf4fbfabc692e81fb6ff892023-04-30T11:11:11ZengBMCEuropean Journal of Medical Research2047-783X2022-09-012711810.1186/s40001-022-00804-xThe effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosaParnia Ghapanvari0Mohammad Taheri1Farid Aziz Jalilian2Sanaz Dehbashi3Aram Asareh Zadegan Dezfuli4Mohammad Reza Arabestani5Microbiology department, Faculty of Medicine, Hamadan University of Medical SciencesMicrobiology department, Faculty of Medicine, Hamadan University of Medical SciencesDepartment of Virology, School of Medicine, University of HamadanMicrobiology department, Faculty of Medicine, Hamadan University of Medical SciencesDepartment of Microbiology, Faculty of Medicine, Ahvaz Jundishapour University of Medical SciencesMicrobiology department, Faculty of Medicine, Hamadan University of Medical SciencesAbstract Objectives Staphylococcus aureus and Pseudomonas aeruginosa were the most common bacteria in nosocomial infections. Different bacteriocins are currently being studied as antibiotics or in conjunction with antibiotics as potential strategies to treat resistant infectious agents. The study aimed to determine nisin's effect on the biofilm production, antimicrobial susceptibility, and biofilm formation of S. aureus and P. aeruginosa. Materials and methods The experimental research tested two antibiotic-resistant isolates of S. aureus and P. aeruginosa strains. The experimental study tested two antibiotic-resistant isolates of S. aureus and P. aeruginosa strains. The MIC of bacteriocin nisin was determined using the micro broth dilution method, and crystal violet was used to assess the effect of bacteriocin on the biofilm. In addition, L929 cell culture was used to determine the effectiveness of bacteriocin on the isolate under similar cell conditions. Moreover, the MTT assay was used to and evaluate bacteriocin toxicity. In this study, the software Prism version 9 and Graph pad software were utilized. Results The results of this study reveal that the nisin has different activities at different doses and is considered dose-dependent. At various times and doses, nisin inhibits biofilm formation in S. aureus, and P. aeruginosa isolates. Nisin also showed a decreasing survival of the isolates. Antibiotic-resistant bacteria can be made more vulnerable by nisin. Furthermore, nisin treatment affected the production of virulence factors such as hemolysins in S. aureus and had little or a negative effect on P. aeruginosa virulence factors. This medication stops S. aureus and P. aeruginosa from growing and causes bacterial cell damage. Conclusions Antibacterial properties of nicin against S. aureus and P. aeruginosa were successfully studied. This bacteriocin stops S. aureus and P. aeruginosa from growing and causes bacterial cell damage or death. Damage to the membrane among the fundamental causes is reduced membrane potential and enzyme inactivation.https://doi.org/10.1186/s40001-022-00804-xBacteriocinNisinStaphylococcus aureusPseudomonas aeruginosaCo-culturePolymicrobial infection |
| spellingShingle | Parnia Ghapanvari Mohammad Taheri Farid Aziz Jalilian Sanaz Dehbashi Aram Asareh Zadegan Dezfuli Mohammad Reza Arabestani The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa European Journal of Medical Research Bacteriocin Nisin Staphylococcus aureus Pseudomonas aeruginosa Co-culture Polymicrobial infection |
| title | The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa |
| title_full | The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa |
| title_fullStr | The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa |
| title_full_unstemmed | The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa |
| title_short | The effect of nisin on the biofilm production, antimicrobial susceptibility and biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa |
| title_sort | effect of nisin on the biofilm production antimicrobial susceptibility and biofilm formation of staphylococcus aureus and pseudomonas aeruginosa |
| topic | Bacteriocin Nisin Staphylococcus aureus Pseudomonas aeruginosa Co-culture Polymicrobial infection |
| url | https://doi.org/10.1186/s40001-022-00804-x |
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