Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in Egypt
A total of 300 human fecal samples were collected from febrile neutropenic patients suffering from severe gastroenteritis, followed by identification and serological characterization of recovered isolates. Fifty nontyphoidal <i>Salmonella</i> (NTS) serovars were recovered. A total of ser...
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MDPI AG
2021-04-01
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| Series: | Antibiotics |
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| author | Reem A. Youssef Ahmad M. Abbas Ahmed M. El-Shehawi Mona I. Mabrouk Khaled M. Aboshanab |
| author_facet | Reem A. Youssef Ahmad M. Abbas Ahmed M. El-Shehawi Mona I. Mabrouk Khaled M. Aboshanab |
| author_sort | Reem A. Youssef |
| collection | DOAJ |
| description | A total of 300 human fecal samples were collected from febrile neutropenic patients suffering from severe gastroenteritis, followed by identification and serological characterization of recovered isolates. Fifty nontyphoidal <i>Salmonella</i> (NTS) serovars were recovered. A total of serologically identified 50 NTS serovars recovered from poultry of the same geographical area and during the same period as well as one standard strain <i>S.</i> Poona were supplied by the Bacterial Bank of Animal Health Research Institute of Egypt. Antibiogram analysis revealed that the human and poultry serovars exhibited similar antimicrobial resistance patterns against 28 different antimicrobial agents, particularly against ampicillin, cefotaxime, oxytetracycline, and erythromycin. Plasmids harboring <i>bla</i><sub>CTX-m</sub>, <i>bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub>, and <i>aac</i>(6’)-Ib were detected in 11 (22%) and 8 (16%) of human and poultry serovars, respectively. Molecular detection of the most clinically relevant virulence genes and analysis of the associated virulence genotypes proved that the human (<i>n</i> = 11) and poultry serovars (<i>n</i> = 12) shared 11 genotypes. Enterobacterial repetitive intergenic consensus PCR analysis revealed that human and poultry serovars were clustered together in 3 out of the 4 clusters with a similarity index ranged from 0.15 to 1. Since poultry are usually consumed by humans, the presence of resistant bacteria harboring transmissible genetic elements is of great health concern. |
| first_indexed | 2024-03-10T11:58:11Z |
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| language | English |
| last_indexed | 2024-03-10T11:58:11Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a640feb687a14716a6a6006308e2fea22023-11-21T17:08:35ZengMDPI AGAntibiotics2079-63822021-04-0110549310.3390/antibiotics10050493Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in EgyptReem A. Youssef0Ahmad M. Abbas1Ahmed M. El-Shehawi2Mona I. Mabrouk3Khaled M. Aboshanab4Department of Microbiology, Egyptian Drug Authority (EDA), P.O. Box 11553, Giza 11553, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., P.O. Box 11566, Cairo 11566, EgyptDepartment of Biotechnology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaDepartment of Microbiology, Egyptian Drug Authority (EDA), P.O. Box 11553, Giza 11553, EgyptDepartment of Microbiology and Immunology, Faculty of Pharmacy, Ain Shams University, Organization of African Unity St., P.O. Box 11566, Cairo 11566, EgyptA total of 300 human fecal samples were collected from febrile neutropenic patients suffering from severe gastroenteritis, followed by identification and serological characterization of recovered isolates. Fifty nontyphoidal <i>Salmonella</i> (NTS) serovars were recovered. A total of serologically identified 50 NTS serovars recovered from poultry of the same geographical area and during the same period as well as one standard strain <i>S.</i> Poona were supplied by the Bacterial Bank of Animal Health Research Institute of Egypt. Antibiogram analysis revealed that the human and poultry serovars exhibited similar antimicrobial resistance patterns against 28 different antimicrobial agents, particularly against ampicillin, cefotaxime, oxytetracycline, and erythromycin. Plasmids harboring <i>bla</i><sub>CTX-m</sub>, <i>bla</i><sub>SHV</sub>, <i>bla</i><sub>TEM</sub>, and <i>aac</i>(6’)-Ib were detected in 11 (22%) and 8 (16%) of human and poultry serovars, respectively. Molecular detection of the most clinically relevant virulence genes and analysis of the associated virulence genotypes proved that the human (<i>n</i> = 11) and poultry serovars (<i>n</i> = 12) shared 11 genotypes. Enterobacterial repetitive intergenic consensus PCR analysis revealed that human and poultry serovars were clustered together in 3 out of the 4 clusters with a similarity index ranged from 0.15 to 1. Since poultry are usually consumed by humans, the presence of resistant bacteria harboring transmissible genetic elements is of great health concern.https://www.mdpi.com/2079-6382/10/5/493nontyphoidal <i>Salmonella</i>serotypingantimicrobial resistancevirulence genesERIC PCR |
| spellingShingle | Reem A. Youssef Ahmad M. Abbas Ahmed M. El-Shehawi Mona I. Mabrouk Khaled M. Aboshanab Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in Egypt Antibiotics nontyphoidal <i>Salmonella</i> serotyping antimicrobial resistance virulence genes ERIC PCR |
| title | Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in Egypt |
| title_full | Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in Egypt |
| title_fullStr | Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in Egypt |
| title_full_unstemmed | Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in Egypt |
| title_short | Serotyping and Antimicrobial Resistance Profile of Enteric Nontyphoidal <i>Salmonella</i> Recovered from Febrile Neutropenic Patients and Poultry in Egypt |
| title_sort | serotyping and antimicrobial resistance profile of enteric nontyphoidal i salmonella i recovered from febrile neutropenic patients and poultry in egypt |
| topic | nontyphoidal <i>Salmonella</i> serotyping antimicrobial resistance virulence genes ERIC PCR |
| url | https://www.mdpi.com/2079-6382/10/5/493 |
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