SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices
The surface-enhanced Raman scattering (SERS) spectra of three amphiphilic oligopeptides derived from EAK16 (AEAEAKAK)<sub>2</sub> were examined to study systematic amino acid substitution effects on the corresponding interaction with Ag colloidal nanoparticles. Such self-assembling molec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/7/959 |
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| author | Michele Di Foggia Vitaliano Tugnoli Stefano Ottani Monica Dettin Annj Zamuner Santiago Sanchez-Cortes Daniele Cesini Armida Torreggiani |
| author_facet | Michele Di Foggia Vitaliano Tugnoli Stefano Ottani Monica Dettin Annj Zamuner Santiago Sanchez-Cortes Daniele Cesini Armida Torreggiani |
| author_sort | Michele Di Foggia |
| collection | DOAJ |
| description | The surface-enhanced Raman scattering (SERS) spectra of three amphiphilic oligopeptides derived from EAK16 (AEAEAKAK)<sub>2</sub> were examined to study systematic amino acid substitution effects on the corresponding interaction with Ag colloidal nanoparticles. Such self-assembling molecular systems, known as “molecular Lego”, are of particular interest for their uses in tissue engineering and as biomimetic coatings for medical devices because they can form insoluble macroscopic membranes under physiological conditions. Spectra were collected for both native and gamma-irradiated samples. Quantum mechanical data on two of the examined oligopeptides were also obtained to clarify the assignment of the prominent significative bands observed in the spectra. In general, the peptide–nanoparticles interaction occurs through the COO<sup>−</sup> groups, with the amide bond and the aliphatic chain close to the colloid surface. After gamma irradiation, mimicking a free oxidative radical attack, the SERS spectra of the biomaterials show that COO<sup>−</sup> groups still provide the main peptide–nanoparticle interactions. However, the spatial arrangement of the peptides is different, exhibiting a systematic decrease in the distance between aliphatic chains and colloid nanoparticles. |
| first_indexed | 2024-03-10T09:57:59Z |
| format | Article |
| id | doaj.art-a6412573c36e48209f1a907247dc9bb8 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T09:57:59Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-a6412573c36e48209f1a907247dc9bb82023-11-22T02:12:28ZengMDPI AGBiomolecules2218-273X2021-06-0111795910.3390/biom11070959SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical DevicesMichele Di Foggia0Vitaliano Tugnoli1Stefano Ottani2Monica Dettin3Annj Zamuner4Santiago Sanchez-Cortes5Daniele Cesini6Armida Torreggiani7Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, 40126 Bologna, ItalyDipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, 40126 Bologna, ItalyIstituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche (ISOF-CNR), 40129 Bologna, ItalyDipartimento di Ingegneria Industriale, Università di Padova, 35131 Padova, ItalyDipartimento di Ingegneria Industriale, Università di Padova, 35131 Padova, ItalyInstituto de Estructura de la Materia, Consejo Superior de Investigaciones Cientificas (CSIC), 28006 Madrid, SpainDipartimento CNAF, Istituto Nazionale di Fisica Nucleare (INFN-CNAF), 40127 Bologna, ItalyIstituto per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale delle Ricerche (ISOF-CNR), 40129 Bologna, ItalyThe surface-enhanced Raman scattering (SERS) spectra of three amphiphilic oligopeptides derived from EAK16 (AEAEAKAK)<sub>2</sub> were examined to study systematic amino acid substitution effects on the corresponding interaction with Ag colloidal nanoparticles. Such self-assembling molecular systems, known as “molecular Lego”, are of particular interest for their uses in tissue engineering and as biomimetic coatings for medical devices because they can form insoluble macroscopic membranes under physiological conditions. Spectra were collected for both native and gamma-irradiated samples. Quantum mechanical data on two of the examined oligopeptides were also obtained to clarify the assignment of the prominent significative bands observed in the spectra. In general, the peptide–nanoparticles interaction occurs through the COO<sup>−</sup> groups, with the amide bond and the aliphatic chain close to the colloid surface. After gamma irradiation, mimicking a free oxidative radical attack, the SERS spectra of the biomaterials show that COO<sup>−</sup> groups still provide the main peptide–nanoparticle interactions. However, the spatial arrangement of the peptides is different, exhibiting a systematic decrease in the distance between aliphatic chains and colloid nanoparticles.https://www.mdpi.com/2218-273X/11/7/959amphiphilic oligopeptidesSERSbiomimetic coatingDFToligopeptide–surface interactionoxidative stress |
| spellingShingle | Michele Di Foggia Vitaliano Tugnoli Stefano Ottani Monica Dettin Annj Zamuner Santiago Sanchez-Cortes Daniele Cesini Armida Torreggiani SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices Biomolecules amphiphilic oligopeptides SERS biomimetic coating DFT oligopeptide–surface interaction oxidative stress |
| title | SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices |
| title_full | SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices |
| title_fullStr | SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices |
| title_full_unstemmed | SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices |
| title_short | SERS Investigation on Oligopeptides Used as Biomimetic Coatings for Medical Devices |
| title_sort | sers investigation on oligopeptides used as biomimetic coatings for medical devices |
| topic | amphiphilic oligopeptides SERS biomimetic coating DFT oligopeptide–surface interaction oxidative stress |
| url | https://www.mdpi.com/2218-273X/11/7/959 |
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