Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface
Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) among animals and humans are a public health threat. This study analyzed the occurrence of ESBL-E in a high-risk environment in a companion animal clinic and two animal patients’ households. In an intensive care unit (ICU), recta...
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| Format: | Article |
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MDPI AG
2021-02-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/2/171 |
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| author | Kira Schmitt Stefan P. Kuster Katrin Zurfluh Rahel S. Jud Jane E. Sykes Roger Stephan Barbara Willi |
| author_facet | Kira Schmitt Stefan P. Kuster Katrin Zurfluh Rahel S. Jud Jane E. Sykes Roger Stephan Barbara Willi |
| author_sort | Kira Schmitt |
| collection | DOAJ |
| description | Extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) among animals and humans are a public health threat. This study analyzed the occurrence of ESBL-E in a high-risk environment in a companion animal clinic and two animal patients’ households. In an intensive care unit (ICU), rectal swabs from 74 dogs and cats, 74 hand swabs from staff and 298 swabs from surfaces were analyzed for ESBL-E. Seventeen hospitalized patients (23%) and ten (3%) surfaces in the ICU tested ESBL-E positive. Transmission chains for <i>Klebsiella pneumoniae</i> ST307 <i>bla</i><sub>CTX-M-15</sub> and <i>Escherichia coli</i> ST38 <i>bla</i><sub>CTX-M-14</sub>, ST88 <i>bla</i><sub>CTX-M-14</sub> and ST224 <i>bla</i><sub>CTX-M-1</sub> were observed over extended periods of time (14 to 30 days) with similar strains isolated from patients and the clinical environment. After discharge, two colonized dogs (dogs 7 and 12) and their household contacts were resampled. Dog 7 tested repeatedly positive for 77 days, dog 12 tested negative; six (24%) surfaces in the household of the persistently colonized dog tested ESBL-E positive. The owner of dog 7 and one of the owners of dog 12 were colonized. Based on whole genome sequencing, isolates from the owners, their dogs and other ICU patients belonged to the same clusters, highlighting the public health importance of ESBL-E in companion animal clinics. |
| first_indexed | 2024-03-09T05:04:18Z |
| format | Article |
| id | doaj.art-a641add6567044babbef681aab633db1 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-09T05:04:18Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a641add6567044babbef681aab633db12023-12-03T12:56:41ZengMDPI AGAntibiotics2079-63822021-02-0110217110.3390/antibiotics10020171Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household InterfaceKira Schmitt0Stefan P. Kuster1Katrin Zurfluh2Rahel S. Jud3Jane E. Sykes4Roger Stephan5Barbara Willi6Institute for Food Safety and Hygiene, University of Zurich, Winterthurerstrasse 272, CH-8057 Zurich, SwitzerlandDivision of Infectious Diseases and Hospital Epidemiology, University Hospital Zurich, University of Zurich, Rämistrasse 100, CH-8091 Zurich, SwitzerlandInstitute for Food Safety and Hygiene, University of Zurich, Winterthurerstrasse 272, CH-8057 Zurich, SwitzerlandDivision of Critical Care Medicine, Department of Small Animals, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandDepartment of Medicine & Epidemiology, University of California-Davis, 2108 Tupper Hall, Davis, CA 95618, USAInstitute for Food Safety and Hygiene, University of Zurich, Winterthurerstrasse 272, CH-8057 Zurich, SwitzerlandClinic for Small Animal Internal Medicine, University of Zurich, Winterthurerstrasse 260, CH-8057 Zurich, SwitzerlandExtended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-E) among animals and humans are a public health threat. This study analyzed the occurrence of ESBL-E in a high-risk environment in a companion animal clinic and two animal patients’ households. In an intensive care unit (ICU), rectal swabs from 74 dogs and cats, 74 hand swabs from staff and 298 swabs from surfaces were analyzed for ESBL-E. Seventeen hospitalized patients (23%) and ten (3%) surfaces in the ICU tested ESBL-E positive. Transmission chains for <i>Klebsiella pneumoniae</i> ST307 <i>bla</i><sub>CTX-M-15</sub> and <i>Escherichia coli</i> ST38 <i>bla</i><sub>CTX-M-14</sub>, ST88 <i>bla</i><sub>CTX-M-14</sub> and ST224 <i>bla</i><sub>CTX-M-1</sub> were observed over extended periods of time (14 to 30 days) with similar strains isolated from patients and the clinical environment. After discharge, two colonized dogs (dogs 7 and 12) and their household contacts were resampled. Dog 7 tested repeatedly positive for 77 days, dog 12 tested negative; six (24%) surfaces in the household of the persistently colonized dog tested ESBL-E positive. The owner of dog 7 and one of the owners of dog 12 were colonized. Based on whole genome sequencing, isolates from the owners, their dogs and other ICU patients belonged to the same clusters, highlighting the public health importance of ESBL-E in companion animal clinics.https://www.mdpi.com/2079-6382/10/2/171antimicrobial resistancemultidrug resistancecaninefelineESBLhome |
| spellingShingle | Kira Schmitt Stefan P. Kuster Katrin Zurfluh Rahel S. Jud Jane E. Sykes Roger Stephan Barbara Willi Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface Antibiotics antimicrobial resistance multidrug resistance canine feline ESBL home |
| title | Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface |
| title_full | Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface |
| title_fullStr | Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface |
| title_full_unstemmed | Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface |
| title_short | Transmission Chains of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae at the Companion Animal Veterinary Clinic–Household Interface |
| title_sort | transmission chains of extended spectrum beta lactamase producing enterobacteriaceae at the companion animal veterinary clinic household interface |
| topic | antimicrobial resistance multidrug resistance canine feline ESBL home |
| url | https://www.mdpi.com/2079-6382/10/2/171 |
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