Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding
Soybean [Glycine max (Linn.) Merr] is a source of plant-based proteins and an essential oilseed crop and industrial raw material. The increase in the demand for soybeans due to societal changes has coincided with the increase in the breeding of soybean varieties with enhanced traits. Earlier gene ed...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1247707/full |
| _version_ | 1797730966786015232 |
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| author | Dan Yao Dan Yao Junming Zhou Aijing Zhang Jiaxin Wang Yixuan Liu Lixue Wang Wenxuan Pi Zihao Li Wenjun Yue Jinliang Cai Huijing Liu Wenyuan Hao Xiangchun Qu |
| author_facet | Dan Yao Dan Yao Junming Zhou Aijing Zhang Jiaxin Wang Yixuan Liu Lixue Wang Wenxuan Pi Zihao Li Wenjun Yue Jinliang Cai Huijing Liu Wenyuan Hao Xiangchun Qu |
| author_sort | Dan Yao |
| collection | DOAJ |
| description | Soybean [Glycine max (Linn.) Merr] is a source of plant-based proteins and an essential oilseed crop and industrial raw material. The increase in the demand for soybeans due to societal changes has coincided with the increase in the breeding of soybean varieties with enhanced traits. Earlier gene editing technologies involved zinc finger nucleases and transcription activator-like effector nucleases, but the third-generation gene editing technology uses clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). The rapid development of CRISPR/Cas9 technology has made it one of the most effective, straightforward, affordable, and user-friendly technologies for targeted gene editing. This review summarizes the application of CRISPR/Cas9 technology in soybean molecular breeding. More specifically, it provides an overview of the genes that have been targeted, the type of editing that occurs, the mechanism of action, and the efficiency of gene editing. Furthermore, suggestions for enhancing and accelerating the molecular breeding of novel soybean varieties with ideal traits (e.g., high yield, high quality, and durable disease resistance) are included. |
| first_indexed | 2024-03-12T11:51:47Z |
| format | Article |
| id | doaj.art-a6463f289bbc4bad833b8a83d05d019c |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-12T11:51:47Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a6463f289bbc4bad833b8a83d05d019c2023-08-31T06:47:12ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-08-011410.3389/fpls.2023.12477071247707Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breedingDan Yao0Dan Yao1Junming Zhou2Aijing Zhang3Jiaxin Wang4Yixuan Liu5Lixue Wang6Wenxuan Pi7Zihao Li8Wenjun Yue9Jinliang Cai10Huijing Liu11Wenyuan Hao12Xiangchun Qu13College of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaInstitute of Crop Resources, Jilin Provincial Academy of Agricultural Sciences, Gongzhuling, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaCollege of Life Science, Jilin Agricultural University, Changchun, Jilin, ChinaJilin Provincial Academy of Agricultural Sciences, Changchun, Jilin, ChinaInstitute of Crop Resources, Jilin Provincial Academy of Agricultural Sciences, Gongzhuling, Jilin, ChinaSoybean [Glycine max (Linn.) Merr] is a source of plant-based proteins and an essential oilseed crop and industrial raw material. The increase in the demand for soybeans due to societal changes has coincided with the increase in the breeding of soybean varieties with enhanced traits. Earlier gene editing technologies involved zinc finger nucleases and transcription activator-like effector nucleases, but the third-generation gene editing technology uses clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). The rapid development of CRISPR/Cas9 technology has made it one of the most effective, straightforward, affordable, and user-friendly technologies for targeted gene editing. This review summarizes the application of CRISPR/Cas9 technology in soybean molecular breeding. More specifically, it provides an overview of the genes that have been targeted, the type of editing that occurs, the mechanism of action, and the efficiency of gene editing. Furthermore, suggestions for enhancing and accelerating the molecular breeding of novel soybean varieties with ideal traits (e.g., high yield, high quality, and durable disease resistance) are included.https://www.frontiersin.org/articles/10.3389/fpls.2023.1247707/fullCRISPR/Cas9soybeanmolecular breedinggene editingapplication |
| spellingShingle | Dan Yao Dan Yao Junming Zhou Aijing Zhang Jiaxin Wang Yixuan Liu Lixue Wang Wenxuan Pi Zihao Li Wenjun Yue Jinliang Cai Huijing Liu Wenyuan Hao Xiangchun Qu Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding Frontiers in Plant Science CRISPR/Cas9 soybean molecular breeding gene editing application |
| title | Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding |
| title_full | Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding |
| title_fullStr | Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding |
| title_full_unstemmed | Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding |
| title_short | Advances in CRISPR/Cas9-based research related to soybean [Glycine max (Linn.) Merr] molecular breeding |
| title_sort | advances in crispr cas9 based research related to soybean glycine max linn merr molecular breeding |
| topic | CRISPR/Cas9 soybean molecular breeding gene editing application |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1247707/full |
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