Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study
Sheep estrous synchronization is mainly based on progestagen-impregnated sponges which could cause vaginitis. Several species of <i>Lactobacillus</i> used as probiotics are commonly used in the treatment or prevention of urogenital infections in humans. However, no studies have been perf...
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| Format: | Article |
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MDPI AG
2020-04-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/4/719 |
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| author | Juan J. Quereda Empar García-Roselló Marta Barba María L. Mocé Jesús Gomis Estrella Jiménez-Trigos Esther Bataller Rebeca Martínez-Boví Ángel García-Muñoz Ángel Gómez-Martín |
| author_facet | Juan J. Quereda Empar García-Roselló Marta Barba María L. Mocé Jesús Gomis Estrella Jiménez-Trigos Esther Bataller Rebeca Martínez-Boví Ángel García-Muñoz Ángel Gómez-Martín |
| author_sort | Juan J. Quereda |
| collection | DOAJ |
| description | Sheep estrous synchronization is mainly based on progestagen-impregnated sponges which could cause vaginitis. Several species of <i>Lactobacillus</i> used as probiotics are commonly used in the treatment or prevention of urogenital infections in humans. However, no studies have been performed to analyze the potential use of probiotics to prevent urogenital infections in sheep. A randomized controlled clinical trial was conducted with 21 one-year-old ewes to develop a model of probiotic infusion in vaginal sponges in order to study their influence in ewe’s vaginal microbiota, general health status, fertility and prolificity. Synchronization of estrus was based on intravaginal sponges for 14 days. Bacterial communities (Enterobacteriaceae and lactic acid bacteria) were highly fluctuating over time and between animals. The safety of probiotic infusion (mix of <i>Lactobacillus</i> spp. 60% <i>L. crispatus</i>, 20% <i>L. brevis</i> and 20% <i>L. gasseri</i>) in the vagina of healthy ewes was firstly confirmed. Neutrophils were observed in 80% (8/10) of the control ewes compared to 36% (4/11) of the ewes in the probiotic group 2 days after sponge removal (<i>p</i> = 0.056). Fertility in the control and probiotic groups was 60% (6/10) and 91% (10/11), respectively <i>p</i> = 0.097. These results suggest that <i>Lactobacillus</i> spp. infusion in the ewe’s vagina does not affect general health status or fertility. |
| first_indexed | 2024-03-10T20:21:30Z |
| format | Article |
| id | doaj.art-a6477902b8474aacbc70e12e2d2ceaab |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T20:21:30Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-a6477902b8474aacbc70e12e2d2ceaab2023-11-19T22:12:00ZengMDPI AGAnimals2076-26152020-04-0110471910.3390/ani10040719Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot StudyJuan J. Quereda0Empar García-Roselló1Marta Barba2María L. Mocé3Jesús Gomis4Estrella Jiménez-Trigos5Esther Bataller6Rebeca Martínez-Boví7Ángel García-Muñoz8Ángel Gómez-Martín9Research Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainResearch Group Microbiological Agents Associated with Animal Reproduction (PROVAGINBIO), Department of Animal Production and Health, Veterinary Public Health and Food Science and Technology (PASAPTA) Facultad de Veterinaria, Universidad Cardenal Herrera-CEU, CEU Universities, 46113 Valencia, SpainSheep estrous synchronization is mainly based on progestagen-impregnated sponges which could cause vaginitis. Several species of <i>Lactobacillus</i> used as probiotics are commonly used in the treatment or prevention of urogenital infections in humans. However, no studies have been performed to analyze the potential use of probiotics to prevent urogenital infections in sheep. A randomized controlled clinical trial was conducted with 21 one-year-old ewes to develop a model of probiotic infusion in vaginal sponges in order to study their influence in ewe’s vaginal microbiota, general health status, fertility and prolificity. Synchronization of estrus was based on intravaginal sponges for 14 days. Bacterial communities (Enterobacteriaceae and lactic acid bacteria) were highly fluctuating over time and between animals. The safety of probiotic infusion (mix of <i>Lactobacillus</i> spp. 60% <i>L. crispatus</i>, 20% <i>L. brevis</i> and 20% <i>L. gasseri</i>) in the vagina of healthy ewes was firstly confirmed. Neutrophils were observed in 80% (8/10) of the control ewes compared to 36% (4/11) of the ewes in the probiotic group 2 days after sponge removal (<i>p</i> = 0.056). Fertility in the control and probiotic groups was 60% (6/10) and 91% (10/11), respectively <i>p</i> = 0.097. These results suggest that <i>Lactobacillus</i> spp. infusion in the ewe’s vagina does not affect general health status or fertility.https://www.mdpi.com/2076-2615/10/4/719lactic acid bacteria<i>Lactobacillus</i>dysbiosisalternative to antibioticsvaginitisestrus synchronization |
| spellingShingle | Juan J. Quereda Empar García-Roselló Marta Barba María L. Mocé Jesús Gomis Estrella Jiménez-Trigos Esther Bataller Rebeca Martínez-Boví Ángel García-Muñoz Ángel Gómez-Martín Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study Animals lactic acid bacteria <i>Lactobacillus</i> dysbiosis alternative to antibiotics vaginitis estrus synchronization |
| title | Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study |
| title_full | Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study |
| title_fullStr | Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study |
| title_full_unstemmed | Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study |
| title_short | Use of Probiotics in Intravaginal Sponges in Sheep: A Pilot Study |
| title_sort | use of probiotics in intravaginal sponges in sheep a pilot study |
| topic | lactic acid bacteria <i>Lactobacillus</i> dysbiosis alternative to antibiotics vaginitis estrus synchronization |
| url | https://www.mdpi.com/2076-2615/10/4/719 |
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