Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers
Rough lemon (Citrus jambhiri Lush) is one of the leading citrus rootstock around the world besides sour orange. In the present studies, rough lemon was crossed with X 639 and Swingle citrumelo at Department of Fruit Science, PAU Ludhiana during 2015-16. Cent per cent seed germination was recorded f...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2020-07-01
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| Series: | The Indian Journal of Agricultural Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAgS/article/view/102232 |
| _version_ | 1811164725465055232 |
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| author | Jagveer Singh H S Dhaliwal Anirudh Thakur G S Sidhu P Chhuneja Kuldeep Pandey |
| author_facet | Jagveer Singh H S Dhaliwal Anirudh Thakur G S Sidhu P Chhuneja Kuldeep Pandey |
| author_sort | Jagveer Singh |
| collection | DOAJ |
| description |
Rough lemon (Citrus jambhiri Lush) is one of the leading citrus rootstock around the world besides sour orange. In the present studies, rough lemon was crossed with X 639 and Swingle citrumelo at Department of Fruit Science, PAU Ludhiana during 2015-16. Cent per cent seed germination was recorded for both the crosses under in vitro conditions but, the germination rates with growing media in plug trays were significantly lower (91.3 and 89.2% for RL× X and RL × SC, respectively). Higher proportion of multi-foliate seedlings (26.89%) was recovered by in vitro seed culture over the ex vitro seed germination (5.20%). All the multi-foliate seedlings were confirmed by SSR analysis using two primers (F 29 and F 87). The SSR analysis showed that only 85% multifoliate seedlings were zygotic while, the rest were not confirmed to be zygotic. The SSR analysis of the 50 unifoliate F1 seedlings each from both the crosses with 5 SSR primers differentiated seedlings into two groups, viz. zygotic and nucellar seedlings. In RL × X cross, the most efficient SSR marker was F29, which identified 30% F1 unifoliate seedlings to be zygotic followed by CCSME31, which identified 26% unifoliate seedlings as zygotic seedlings while the remaining seedlings were nucellar. In the cross RL × SC, the primer F29 was most efficient as it identified 28% zygotic seedlings and 72% nucellar seedlings whereas, the primer F87 identified 24 and 76% zygotic and nucellar seedlings, respectively.
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| first_indexed | 2024-04-10T15:26:07Z |
| format | Article |
| id | doaj.art-a64c8884fffc4ba190b176c88e533468 |
| institution | Directory Open Access Journal |
| issn | 0019-5022 2394-3319 |
| language | English |
| last_indexed | 2024-04-10T15:26:07Z |
| publishDate | 2020-07-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | The Indian Journal of Agricultural Sciences |
| spelling | doaj.art-a64c8884fffc4ba190b176c88e5334682023-02-14T09:09:53ZengIndian Council of Agricultural ResearchThe Indian Journal of Agricultural Sciences0019-50222394-33192020-07-0190410.56093/ijas.v90i4.102232Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markersJagveer Singh0H S Dhaliwal1Anirudh Thakur2G S Sidhu3P Chhuneja4Kuldeep Pandey5Punjab Agricultural University, Ludhiana, Punjab 141 004, IndiaPunjab Agricultural University, Ludhiana, Punjab 141 004, IndiaPunjab Agricultural University, Ludhiana, Punjab 141 004, IndiaPunjab Agricultural University, Ludhiana, Punjab 141 004, IndiaPunjab Agricultural University, Ludhiana, Punjab 141 004, IndiaPunjab Agricultural University, Ludhiana, Punjab 141 004, India Rough lemon (Citrus jambhiri Lush) is one of the leading citrus rootstock around the world besides sour orange. In the present studies, rough lemon was crossed with X 639 and Swingle citrumelo at Department of Fruit Science, PAU Ludhiana during 2015-16. Cent per cent seed germination was recorded for both the crosses under in vitro conditions but, the germination rates with growing media in plug trays were significantly lower (91.3 and 89.2% for RL× X and RL × SC, respectively). Higher proportion of multi-foliate seedlings (26.89%) was recovered by in vitro seed culture over the ex vitro seed germination (5.20%). All the multi-foliate seedlings were confirmed by SSR analysis using two primers (F 29 and F 87). The SSR analysis showed that only 85% multifoliate seedlings were zygotic while, the rest were not confirmed to be zygotic. The SSR analysis of the 50 unifoliate F1 seedlings each from both the crosses with 5 SSR primers differentiated seedlings into two groups, viz. zygotic and nucellar seedlings. In RL × X cross, the most efficient SSR marker was F29, which identified 30% F1 unifoliate seedlings to be zygotic followed by CCSME31, which identified 26% unifoliate seedlings as zygotic seedlings while the remaining seedlings were nucellar. In the cross RL × SC, the primer F29 was most efficient as it identified 28% zygotic seedlings and 72% nucellar seedlings whereas, the primer F87 identified 24 and 76% zygotic and nucellar seedlings, respectively. https://epubs.icar.org.in/index.php/IJAgS/article/view/102232Citrus breedingEmbryo rescueMulti-foliate seedlings and rootstock. |
| spellingShingle | Jagveer Singh H S Dhaliwal Anirudh Thakur G S Sidhu P Chhuneja Kuldeep Pandey Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers The Indian Journal of Agricultural Sciences Citrus breeding Embryo rescue Multi-foliate seedlings and rootstock. |
| title | Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers |
| title_full | Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers |
| title_fullStr | Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers |
| title_full_unstemmed | Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers |
| title_short | Ex vitro recovery of rough lemon (Citrus jambhiri) hybrids and identification with SSR markers |
| title_sort | ex vitro recovery of rough lemon citrus jambhiri hybrids and identification with ssr markers |
| topic | Citrus breeding Embryo rescue Multi-foliate seedlings and rootstock. |
| url | https://epubs.icar.org.in/index.php/IJAgS/article/view/102232 |
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