Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based Analysis
This study aimed to elucidate the metabolomic signature associated with obesity and periodontitis during pregnancy in plasma and saliva biofluids. Ninety-eight pregnant women were divided into: with obesity and periodontitis (OP = 20), with obesity but without periodontitis (OWP = 27), with normal B...
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MDPI AG
2022-10-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/12/11/1029 |
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| author | Gerson Aparecido Foratori-Junior Adrien Le Guennec Tatiana Kelly da Silva Fidalgo Leanne Cleaver Marília Afonso Rabelo Buzalaf Guy Howard Carpenter Silvia Helena de Carvalho Sales-Peres |
| author_facet | Gerson Aparecido Foratori-Junior Adrien Le Guennec Tatiana Kelly da Silva Fidalgo Leanne Cleaver Marília Afonso Rabelo Buzalaf Guy Howard Carpenter Silvia Helena de Carvalho Sales-Peres |
| author_sort | Gerson Aparecido Foratori-Junior |
| collection | DOAJ |
| description | This study aimed to elucidate the metabolomic signature associated with obesity and periodontitis during pregnancy in plasma and saliva biofluids. Ninety-eight pregnant women were divided into: with obesity and periodontitis (OP = 20), with obesity but without periodontitis (OWP = 27), with normal BMI but with periodontitis (NP = 21), with normal BMI and without periodontitis (NWP = 30). Saliva and plasma were analyzed by <sup>1</sup>H-NMR for metabolites identification. Partial Least Squares-Discriminant Analysis (PLS-DA), Sparse PLS-DA (sPLS-DA), and Variable Importance of Projection (VIP) were performed. ANOVA and Pearson’s correlation were applied (<i>p</i> < 0.05). Plasmatic analysis indicated the levels of glucose (<i>p</i> = 0.041) and phenylalanine (<i>p</i> = 0.015) were positively correlated with periodontal parameters and BMI, respectively. In saliva, periodontitis was mainly associated with high levels of acetic acid (<i>p</i> = 0.024), isovaleric acid, butyric acid, leucine, valine, isoleucine, and propionic acid (<i>p</i> < 0.001). High salivary concentrations of glycine (<i>p</i> = 0.015), succinic acid (<i>p</i> = 0.015), and lactate (<i>p</i> = 0.026) were associated with obesity. Saliva demonstrated a more elucidative difference than plasma, indicating the glucose-alanine cycle, alanine metabolism, valine, leucine and isoleucine degradation, glutamate metabolism, and Warburg effect as the main metabolic pathways. |
| first_indexed | 2024-03-09T18:51:07Z |
| format | Article |
| id | doaj.art-a64f7aa7536b4511b7cd5111b68f7c65 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-09T18:51:07Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-a64f7aa7536b4511b7cd5111b68f7c652023-11-24T05:49:53ZengMDPI AGMetabolites2218-19892022-10-011211102910.3390/metabo12111029Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based AnalysisGerson Aparecido Foratori-Junior0Adrien Le Guennec1Tatiana Kelly da Silva Fidalgo2Leanne Cleaver3Marília Afonso Rabelo Buzalaf4Guy Howard Carpenter5Silvia Helena de Carvalho Sales-Peres6Department of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, BrazilNuclear Magnetic Resonance Facility, Guy’s Campus, King’s College London, London SE1 1UL, UKDepartment of Preventive and Community Dentistry, School of Dentistry, Rio de Janeiro State University, Rio de Janeiro 20551-030, BrazilCentre for Host-Microbiome Interactions, Faculty of Dental, Oral & Craniofacial Sciences, Guy’s Campus, King’s College London, London SE1 1UL, UKDepartment of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, BrazilCentre for Host-Microbiome Interactions, Faculty of Dental, Oral & Craniofacial Sciences, Guy’s Campus, King’s College London, London SE1 1UL, UKDepartment of Pediatric Dentistry, Orthodontics and Public Health, Bauru School of Dentistry, University of São Paulo, Bauru 17012-901, BrazilThis study aimed to elucidate the metabolomic signature associated with obesity and periodontitis during pregnancy in plasma and saliva biofluids. Ninety-eight pregnant women were divided into: with obesity and periodontitis (OP = 20), with obesity but without periodontitis (OWP = 27), with normal BMI but with periodontitis (NP = 21), with normal BMI and without periodontitis (NWP = 30). Saliva and plasma were analyzed by <sup>1</sup>H-NMR for metabolites identification. Partial Least Squares-Discriminant Analysis (PLS-DA), Sparse PLS-DA (sPLS-DA), and Variable Importance of Projection (VIP) were performed. ANOVA and Pearson’s correlation were applied (<i>p</i> < 0.05). Plasmatic analysis indicated the levels of glucose (<i>p</i> = 0.041) and phenylalanine (<i>p</i> = 0.015) were positively correlated with periodontal parameters and BMI, respectively. In saliva, periodontitis was mainly associated with high levels of acetic acid (<i>p</i> = 0.024), isovaleric acid, butyric acid, leucine, valine, isoleucine, and propionic acid (<i>p</i> < 0.001). High salivary concentrations of glycine (<i>p</i> = 0.015), succinic acid (<i>p</i> = 0.015), and lactate (<i>p</i> = 0.026) were associated with obesity. Saliva demonstrated a more elucidative difference than plasma, indicating the glucose-alanine cycle, alanine metabolism, valine, leucine and isoleucine degradation, glutamate metabolism, and Warburg effect as the main metabolic pathways.https://www.mdpi.com/2218-1989/12/11/1029obesityperiodontitispregnancymetabolomicssaliva |
| spellingShingle | Gerson Aparecido Foratori-Junior Adrien Le Guennec Tatiana Kelly da Silva Fidalgo Leanne Cleaver Marília Afonso Rabelo Buzalaf Guy Howard Carpenter Silvia Helena de Carvalho Sales-Peres Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based Analysis Metabolites obesity periodontitis pregnancy metabolomics saliva |
| title | Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based Analysis |
| title_full | Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based Analysis |
| title_fullStr | Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based Analysis |
| title_full_unstemmed | Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based Analysis |
| title_short | Metabolomic Profiles Associated with Obesity and Periodontitis during Pregnancy: Cross-Sectional Study with Proton Nuclear Magnetic Resonance (<sup>1</sup>H-NMR)-Based Analysis |
| title_sort | metabolomic profiles associated with obesity and periodontitis during pregnancy cross sectional study with proton nuclear magnetic resonance sup 1 sup h nmr based analysis |
| topic | obesity periodontitis pregnancy metabolomics saliva |
| url | https://www.mdpi.com/2218-1989/12/11/1029 |
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