A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains
Aflatoxin B1 (AFB1) is a toxic compound naturally produced by the genera <i>Aspergillus</i>. Distillers’ grains can be used as animal feed since they have high content of crude protein and other nutrients. However, they are easily contaminated by mycotoxins, and currently there are no ra...
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MDPI AG
2021-09-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/9/2109 |
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| author | Zifei Wang Pengjie Luo Baodong Zheng |
| author_facet | Zifei Wang Pengjie Luo Baodong Zheng |
| author_sort | Zifei Wang |
| collection | DOAJ |
| description | Aflatoxin B1 (AFB1) is a toxic compound naturally produced by the genera <i>Aspergillus</i>. Distillers’ grains can be used as animal feed since they have high content of crude protein and other nutrients. However, they are easily contaminated by mycotoxins, and currently there are no rapid detection methods for AFB1 in distillers’ grains. In this study, a lateral flow immunoassay (LFIA) based on red fluorescent microsphere (FM), is developed for quantitative detection of AFB1 in distillers’ grains. The whole test can be completed within 15 min, with the cut-off value being 25.0 μg/kg, and the quantitative limit of detection (qLOD) being 3.4 μg/kg. This method represents satisfactory recoveries of 95.2–113.0%, and the coefficients of variation (CVs) are less than 7.0%. Furthermore, this technique is successfully used to analyze AFB1 in real samples, and the results indicates good consistency with that of high-performance liquid chromatography (HPLC). The correlation coefficient is found to be greater than 0.99. The proposed test strip facilitates on-site, cost-effective, and sensitive monitoring of AFB1 in distillers’ grains. |
| first_indexed | 2024-03-10T07:40:52Z |
| format | Article |
| id | doaj.art-a650abf3223f45d98fb30ec206e1ff97 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T07:40:52Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
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| series | Foods |
| spelling | doaj.art-a650abf3223f45d98fb30ec206e1ff972023-11-22T13:04:22ZengMDPI AGFoods2304-81582021-09-01109210910.3390/foods10092109A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ GrainsZifei Wang0Pengjie Luo1Baodong Zheng2College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaChinese Academy of Medical Science Research Unit (No. 2019RU014), NHC Key Laboratory of Food Safety Risk Assessment, China National Center for Food Safety Risk Assessment, Beijing 100022, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaAflatoxin B1 (AFB1) is a toxic compound naturally produced by the genera <i>Aspergillus</i>. Distillers’ grains can be used as animal feed since they have high content of crude protein and other nutrients. However, they are easily contaminated by mycotoxins, and currently there are no rapid detection methods for AFB1 in distillers’ grains. In this study, a lateral flow immunoassay (LFIA) based on red fluorescent microsphere (FM), is developed for quantitative detection of AFB1 in distillers’ grains. The whole test can be completed within 15 min, with the cut-off value being 25.0 μg/kg, and the quantitative limit of detection (qLOD) being 3.4 μg/kg. This method represents satisfactory recoveries of 95.2–113.0%, and the coefficients of variation (CVs) are less than 7.0%. Furthermore, this technique is successfully used to analyze AFB1 in real samples, and the results indicates good consistency with that of high-performance liquid chromatography (HPLC). The correlation coefficient is found to be greater than 0.99. The proposed test strip facilitates on-site, cost-effective, and sensitive monitoring of AFB1 in distillers’ grains.https://www.mdpi.com/2304-8158/10/9/2109aflatoxin B1distillers’ grainsfluorescent microsphereslateral flow immunoassay |
| spellingShingle | Zifei Wang Pengjie Luo Baodong Zheng A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains Foods aflatoxin B1 distillers’ grains fluorescent microspheres lateral flow immunoassay |
| title | A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains |
| title_full | A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains |
| title_fullStr | A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains |
| title_full_unstemmed | A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains |
| title_short | A Rapid and Sensitive Fluorescent Microsphere-Based Lateral Flow Immunoassay for Determination of Aflatoxin B1 in Distillers’ Grains |
| title_sort | rapid and sensitive fluorescent microsphere based lateral flow immunoassay for determination of aflatoxin b1 in distillers grains |
| topic | aflatoxin B1 distillers’ grains fluorescent microspheres lateral flow immunoassay |
| url | https://www.mdpi.com/2304-8158/10/9/2109 |
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