Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERS
The aim of this study is to develop a method for the determination of chlormequat (CCC) residues in grain and oil. The pretreatment by using QuEChERS and Gas Chromatography-Tandem Massspectrometry (GC-MS/MS) analysis were optimized. The samples were extracted from wheat flour, rice, corn, soybean, s...
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| Format: | Article |
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Academy of National Food and Strategic Reserves Administration
2022-09-01
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| Series: | Liang you shipin ke-ji |
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| Online Access: | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220528?st=article_issue |
| _version_ | 1797869991559692288 |
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| author | LIU Fu-ying YANG Shui-yan GUO Ying SHAO Zi-lin |
| author_facet | LIU Fu-ying YANG Shui-yan GUO Ying SHAO Zi-lin |
| author_sort | LIU Fu-ying |
| collection | DOAJ |
| description | The aim of this study is to develop a method for the determination of chlormequat (CCC) residues in grain and oil. The pretreatment by using QuEChERS and Gas Chromatography-Tandem Massspectrometry (GC-MS/MS) analysis were optimized. The samples were extracted from wheat flour, rice, corn, soybean, soybean oil, rapeseed, and rapeseed oil with acetonitrile and salted out with anhydrous magnesium sulfate, and sodium chloride, then cleaned up by a mixture of N-propyl ethylenediamine (PSA) and octadecyl silane (C18) adsorbents. The matrix effects of different substrates (wheat flour, rice, rapeseed, rapeseed oil) were investigated by derived mass spectrometry and matrix-matched calibration combined with external standards was used for quantitative analysis. The results showed a good linearity in the range of 0.05~2 μg/mL for CCC in different matrixes, with correlation coefficients (R2) of more than 0.9980. The limits of detection (LODs) and the limits of quantification (LOQs) of the method were 0.004~0.009 mg/kg and 0.01~0.03 mg/kg, respectively. The averagerecoveries at three spiked levels were 73.6%~99.1%, and the relative standard deviations (RSDs) were 2.34%~7.78% (n = 6). The method was efficient and sensitive, and satisfied the testing requirements for chlormequat residues in grain and oil samples. |
| first_indexed | 2024-04-10T00:20:18Z |
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| id | doaj.art-a6553a35eb5b4d929b98c4fb010240d1 |
| institution | Directory Open Access Journal |
| issn | 1007-7561 |
| language | English |
| last_indexed | 2024-04-10T00:20:18Z |
| publishDate | 2022-09-01 |
| publisher | Academy of National Food and Strategic Reserves Administration |
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| series | Liang you shipin ke-ji |
| spelling | doaj.art-a6553a35eb5b4d929b98c4fb010240d12023-03-16T03:05:49ZengAcademy of National Food and Strategic Reserves AdministrationLiang you shipin ke-ji1007-75612022-09-0130523424310.16210/j.cnki.1007-7561.2022.05.021Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERSLIU Fu-ying0YANG Shui-yan1GUO Ying2SHAO Zi-lin3Yunnan Grain and Oil Science Research Institute (Yunnan Grain and Oil Product Quality Supervision and Inspection Center), Kunming, Yunnan 650033, ChinaYunnan Grain and Oil Science Research Institute (Yunnan Grain and Oil Product Quality Supervision and Inspection Center), Kunming, Yunnan 650033, ChinaYunnan Grain and Oil Science Research Institute (Yunnan Grain and Oil Product Quality Supervision and Inspection Center), Kunming, Yunnan 650033, ChinaYunnan Grain and Oil Science Research Institute (Yunnan Grain and Oil Product Quality Supervision and Inspection Center), Kunming, Yunnan 650033, ChinaThe aim of this study is to develop a method for the determination of chlormequat (CCC) residues in grain and oil. The pretreatment by using QuEChERS and Gas Chromatography-Tandem Massspectrometry (GC-MS/MS) analysis were optimized. The samples were extracted from wheat flour, rice, corn, soybean, soybean oil, rapeseed, and rapeseed oil with acetonitrile and salted out with anhydrous magnesium sulfate, and sodium chloride, then cleaned up by a mixture of N-propyl ethylenediamine (PSA) and octadecyl silane (C18) adsorbents. The matrix effects of different substrates (wheat flour, rice, rapeseed, rapeseed oil) were investigated by derived mass spectrometry and matrix-matched calibration combined with external standards was used for quantitative analysis. The results showed a good linearity in the range of 0.05~2 μg/mL for CCC in different matrixes, with correlation coefficients (R2) of more than 0.9980. The limits of detection (LODs) and the limits of quantification (LOQs) of the method were 0.004~0.009 mg/kg and 0.01~0.03 mg/kg, respectively. The averagerecoveries at three spiked levels were 73.6%~99.1%, and the relative standard deviations (RSDs) were 2.34%~7.78% (n = 6). The method was efficient and sensitive, and satisfied the testing requirements for chlormequat residues in grain and oil samples.http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220528?st=article_issuegas chromatography-tandem mass spectrometry (gc-ms-ms)grainoilchlormequat (ccc) |
| spellingShingle | LIU Fu-ying YANG Shui-yan GUO Ying SHAO Zi-lin Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERS Liang you shipin ke-ji gas chromatography-tandem mass spectrometry (gc-ms-ms) grain oil chlormequat (ccc) |
| title | Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERS |
| title_full | Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERS |
| title_fullStr | Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERS |
| title_full_unstemmed | Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERS |
| title_short | Determination of Chlormequat Residues in Grains and Oils by Gas Chromatography-Tandem Massspectrometry with QuEChERS |
| title_sort | determination of chlormequat residues in grains and oils by gas chromatography tandem massspectrometry with quechers |
| topic | gas chromatography-tandem mass spectrometry (gc-ms-ms) grain oil chlormequat (ccc) |
| url | http://lyspkj.ijournal.cn/lyspkj/article/abstract/20220528?st=article_issue |
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