Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i>
During the course of our ongoing work to discover new inhibitors of biofilm formation of <i>Staphylococcus aureus</i> from fungal sources, we observed biofilm inhibition by cytochalasans isolated from cultures of the ascomycete <i>Hypoxylon fragiforme</i> for the first time....
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-10-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/8/4/129 |
| _version_ | 1819080137574973440 |
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| author | Kamila Tomoko Yuyama Lucile Wendt Frank Surup Robin Kretz Clara Chepkirui Kathrin Wittstein Chollaratt Boonlarppradab Sarunyou Wongkanoun Jennifer Luangsa-ard Marc Stadler Wolf-Rainer Abraham |
| author_facet | Kamila Tomoko Yuyama Lucile Wendt Frank Surup Robin Kretz Clara Chepkirui Kathrin Wittstein Chollaratt Boonlarppradab Sarunyou Wongkanoun Jennifer Luangsa-ard Marc Stadler Wolf-Rainer Abraham |
| author_sort | Kamila Tomoko Yuyama |
| collection | DOAJ |
| description | During the course of our ongoing work to discover new inhibitors of biofilm formation of <i>Staphylococcus aureus</i> from fungal sources, we observed biofilm inhibition by cytochalasans isolated from cultures of the ascomycete <i>Hypoxylon fragiforme</i> for the first time. Two new compounds were purified by a bioassay-guided fractionation procedure; their structures were elucidated subsequently by nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HR-MS). This unexpected finding prompted us to test further cytochalasans from other fungi and from commercial sources for comparison. Out of 21 cytochalasans, 13 showed significant inhibition of <i>Staphylococcus aureus</i> biofilm formation at subtoxic levels. These findings indicate the potential of cytochalasans as biofilm inhibitors for the first time, also because the minimum inhibitory concentrations (MIC) are independent of the anti-biofilm activities. However, cytochalasans are known to be inhibitors of actin, making some of them very toxic for eukaryotic cells. Since the chemical structures of the tested compounds were rather diverse, the inclusion of additional derivatives, as well as the evaluation of their selectivity against mammalian cells vs. the bacterium, will be necessary as next step in order to develop structure-activity relationships and identify the optimal candidates for development of an anti-biofilm agent. |
| first_indexed | 2024-12-21T19:40:06Z |
| format | Article |
| id | doaj.art-a65d3b900e954cc8930224b71ce2b5fe |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-12-21T19:40:06Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-a65d3b900e954cc8930224b71ce2b5fe2022-12-21T18:52:30ZengMDPI AGBiomolecules2218-273X2018-10-018412910.3390/biom8040129biom8040129Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i>Kamila Tomoko Yuyama0Lucile Wendt1Frank Surup2Robin Kretz3Clara Chepkirui4Kathrin Wittstein5Chollaratt Boonlarppradab6Sarunyou Wongkanoun7Jennifer Luangsa-ard8Marc Stadler9Wolf-Rainer Abraham10Department Chemical Microbiology, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDepartment Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDepartment Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDepartment Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDepartment Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDepartment Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyNational Centre for Genetic Engineering and Biotechnology (BIOTEC), NSTDA, 113 Thailand Science Park, Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, ThailandNational Centre for Genetic Engineering and Biotechnology (BIOTEC), NSTDA, 113 Thailand Science Park, Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, ThailandNational Centre for Genetic Engineering and Biotechnology (BIOTEC), NSTDA, 113 Thailand Science Park, Phahonyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120, ThailandDepartment Microbial Drugs, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDepartment Chemical Microbiology, Helmholtz Centre for Infection Research (HZI), Inhoffenstraße 7, 38124 Braunschweig, GermanyDuring the course of our ongoing work to discover new inhibitors of biofilm formation of <i>Staphylococcus aureus</i> from fungal sources, we observed biofilm inhibition by cytochalasans isolated from cultures of the ascomycete <i>Hypoxylon fragiforme</i> for the first time. Two new compounds were purified by a bioassay-guided fractionation procedure; their structures were elucidated subsequently by nuclear magnetic resonance (NMR) spectroscopy and high-resolution mass spectrometry (HR-MS). This unexpected finding prompted us to test further cytochalasans from other fungi and from commercial sources for comparison. Out of 21 cytochalasans, 13 showed significant inhibition of <i>Staphylococcus aureus</i> biofilm formation at subtoxic levels. These findings indicate the potential of cytochalasans as biofilm inhibitors for the first time, also because the minimum inhibitory concentrations (MIC) are independent of the anti-biofilm activities. However, cytochalasans are known to be inhibitors of actin, making some of them very toxic for eukaryotic cells. Since the chemical structures of the tested compounds were rather diverse, the inclusion of additional derivatives, as well as the evaluation of their selectivity against mammalian cells vs. the bacterium, will be necessary as next step in order to develop structure-activity relationships and identify the optimal candidates for development of an anti-biofilm agent.https://www.mdpi.com/2218-273X/8/4/129ascomycotabacterial pathogensbiofilm dispersionchromatographynatural productssecondary metabolitesstructure elucidationXylariales |
| spellingShingle | Kamila Tomoko Yuyama Lucile Wendt Frank Surup Robin Kretz Clara Chepkirui Kathrin Wittstein Chollaratt Boonlarppradab Sarunyou Wongkanoun Jennifer Luangsa-ard Marc Stadler Wolf-Rainer Abraham Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i> Biomolecules ascomycota bacterial pathogens biofilm dispersion chromatography natural products secondary metabolites structure elucidation Xylariales |
| title | Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i> |
| title_full | Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i> |
| title_fullStr | Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i> |
| title_short | Cytochalasans Act as Inhibitors of Biofilm Formation of <i>Staphylococcus aureus</i> |
| title_sort | cytochalasans act as inhibitors of biofilm formation of i staphylococcus aureus i |
| topic | ascomycota bacterial pathogens biofilm dispersion chromatography natural products secondary metabolites structure elucidation Xylariales |
| url | https://www.mdpi.com/2218-273X/8/4/129 |
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