A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species
Canine brucellosis is primarily caused by Brucella canis, but other Brucella species can also cause the disease. Identifying sequences specific to B. canis and establishing PCR assays that can distinguish between B. canis and other Brucella species is essential to determine the etiology of canine br...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2022.983482/full |
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| author | Yin-Bo Ye Jiang-Hua Yang Dong-Liang Li Li-Hua Hao Zhao Zhang Si-Yao Mei Huan Zhang Fang-Yuan Du Li-Hui Yv Bao-Shan Liu Ze-Liang Chen |
| author_facet | Yin-Bo Ye Jiang-Hua Yang Dong-Liang Li Li-Hua Hao Zhao Zhang Si-Yao Mei Huan Zhang Fang-Yuan Du Li-Hui Yv Bao-Shan Liu Ze-Liang Chen |
| author_sort | Yin-Bo Ye |
| collection | DOAJ |
| description | Canine brucellosis is primarily caused by Brucella canis, but other Brucella species can also cause the disease. Identifying sequences specific to B. canis and establishing PCR assays that can distinguish between B. canis and other Brucella species is essential to determine the etiology of canine brucellosis and the source of infection and to achieve effective control. We analyzed the gaps and SNPs of genomes I and II from B. canis strain RM6/66 and B. melitensis strain 16M using the Mauve genome alignment software, and the specificity of each of these differential regions was analyzed by BLAST. A 132 bp specific sequence was found between the DK60_915 (glycosyl hydrolase 108 family protein) and DK60_917 (aldose 1-epimerase) loci in B. canis chromosome 1. Further comparative analysis revealed that this is a reverse complement sequence between B. canis and other Brucella species. Then, three primers were designed based on the sequence that could detect B. canis with a 310 bp amplification product or other Brucella species with a 413 bp product. The PCR based on these primers had reasonable specificity and a sensitivity of 100 copies of Brucella DNA. The detection results for the blood samples of the aborted dogs showed a favorable accordance with the Bruce-ladder multiplex PCR assay. In conclusion, we found a specific reverse complement sequence between B. canis and other Brucella and developed a PCR method that allows a more comprehensive identification of the pathogen involved in canine brucellosis. These findings provide an effective means for preventing and controlling brucellosis. |
| first_indexed | 2024-04-13T17:10:16Z |
| format | Article |
| id | doaj.art-a65dceaa1fd84e4482bd14f5f8e2919c |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-04-13T17:10:16Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-a65dceaa1fd84e4482bd14f5f8e2919c2022-12-22T02:38:19ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692022-11-01910.3389/fvets.2022.983482983482A specific reverse complement sequence for distinguishing Brucella canis from other Brucella speciesYin-Bo Ye0Jiang-Hua Yang1Dong-Liang Li2Li-Hua Hao3Zhao Zhang4Si-Yao Mei5Huan Zhang6Fang-Yuan Du7Li-Hui Yv8Bao-Shan Liu9Ze-Liang Chen10Key Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaBeijing Animal Disease Prevention and Control Center, Beijing, ChinaDivision of the Standards, China Institute of Veterinary Drug Control, Beijing, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Livestock Infectious Diseases, Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaCanine brucellosis is primarily caused by Brucella canis, but other Brucella species can also cause the disease. Identifying sequences specific to B. canis and establishing PCR assays that can distinguish between B. canis and other Brucella species is essential to determine the etiology of canine brucellosis and the source of infection and to achieve effective control. We analyzed the gaps and SNPs of genomes I and II from B. canis strain RM6/66 and B. melitensis strain 16M using the Mauve genome alignment software, and the specificity of each of these differential regions was analyzed by BLAST. A 132 bp specific sequence was found between the DK60_915 (glycosyl hydrolase 108 family protein) and DK60_917 (aldose 1-epimerase) loci in B. canis chromosome 1. Further comparative analysis revealed that this is a reverse complement sequence between B. canis and other Brucella species. Then, three primers were designed based on the sequence that could detect B. canis with a 310 bp amplification product or other Brucella species with a 413 bp product. The PCR based on these primers had reasonable specificity and a sensitivity of 100 copies of Brucella DNA. The detection results for the blood samples of the aborted dogs showed a favorable accordance with the Bruce-ladder multiplex PCR assay. In conclusion, we found a specific reverse complement sequence between B. canis and other Brucella and developed a PCR method that allows a more comprehensive identification of the pathogen involved in canine brucellosis. These findings provide an effective means for preventing and controlling brucellosis.https://www.frontiersin.org/articles/10.3389/fvets.2022.983482/fullcanine brucellosisB. canisreverse complementary sequencecaninespecific |
| spellingShingle | Yin-Bo Ye Jiang-Hua Yang Dong-Liang Li Li-Hua Hao Zhao Zhang Si-Yao Mei Huan Zhang Fang-Yuan Du Li-Hui Yv Bao-Shan Liu Ze-Liang Chen A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species Frontiers in Veterinary Science canine brucellosis B. canis reverse complementary sequence canine specific |
| title | A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species |
| title_full | A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species |
| title_fullStr | A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species |
| title_full_unstemmed | A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species |
| title_short | A specific reverse complement sequence for distinguishing Brucella canis from other Brucella species |
| title_sort | specific reverse complement sequence for distinguishing brucella canis from other brucella species |
| topic | canine brucellosis B. canis reverse complementary sequence canine specific |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2022.983482/full |
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