Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes
Abstract Purpose This study aimed to evaluate the protective effects of gentiopicroside against lipopolysaccharide-induced chondrocyte inflammation. Methods SW 1353 chondrosarcoma cells were stimulated with LPS (5 μg/ml) for 24 h and treated with different concentrations of gentiopicroside (GPS) for...
| Main Authors: | , , , , , , |
|---|---|
| Formato: | Artigo |
| Idioma: | English |
| Publicado em: |
BMC
2024-03-01
|
| Colecção: | Journal of Orthopaedic Surgery and Research |
| Assuntos: | |
| Acesso em linha: | https://doi.org/10.1186/s13018-024-04676-1 |
| _version_ | 1827301069311442944 |
|---|---|
| author | Longfei Li Qianqian Fan Yixuan Zhao Qian Zhang Gaofeng Qin Chen Li Wei Li |
| author_facet | Longfei Li Qianqian Fan Yixuan Zhao Qian Zhang Gaofeng Qin Chen Li Wei Li |
| author_sort | Longfei Li |
| collection | DOAJ |
| description | Abstract Purpose This study aimed to evaluate the protective effects of gentiopicroside against lipopolysaccharide-induced chondrocyte inflammation. Methods SW 1353 chondrosarcoma cells were stimulated with LPS (5 μg/ml) for 24 h and treated with different concentrations of gentiopicroside (GPS) for 24 h. The toxic effects of GPS on chondrocytes were determined using a CCK-8 assay and EdU staining. Western blotting, qPCR, and immunofluorescence analysis were used to examine the protective effect of GPS against the inflammatory response in chondrocytes induced by lipopolysaccharide (LPS). One-way ANOVA was used to compare the differences between the groups (significance level of 0.05). Results The CCK-8 results showed that 10, 20 and 40 μM GPS had no significant toxic effects on chondrocytes; GPS effectively reduced the production of IL-1β and PGE2, reversed LPS-induced extracellular matrix degradation in cartilage by inhibiting the Stat3/Runx2 signaling pathway, and suppressed the hypertrophic transformation of SW 1353 chondrosarcoma cells. Conclusion Our study demonstrated that GPS significantly inhibited the LPS-induced inflammatory response and hypertrophic cellular degeneration in SW 1353 chondrosarcoma cells and is a valuable traditional Chinese medicine for the treatment of knee osteoarthritis. |
| first_indexed | 2024-04-24T16:16:48Z |
| format | Article |
| id | doaj.art-a65f1533d11a4d96ac5b5b02f81c1e8f |
| institution | Directory Open Access Journal |
| issn | 1749-799X |
| language | English |
| last_indexed | 2024-04-24T16:16:48Z |
| publishDate | 2024-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj.art-a65f1533d11a4d96ac5b5b02f81c1e8f2024-03-31T11:26:47ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-03-0119111010.1186/s13018-024-04676-1Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytesLongfei Li0Qianqian Fan1Yixuan Zhao2Qian Zhang3Gaofeng Qin4Chen Li5Wei Li6School of Special Education and Rehabilitation, Binzhou Medical UniversityDepartment of Rehabilitation, Binzhou Medical University HospitalSchool of Special Education and Rehabilitation, Binzhou Medical UniversitySchool of Special Education and Rehabilitation, Binzhou Medical UniversityDepartment of Rehabilitation, Binzhou Medical University HospitalDepartment of Rehabilitation, Binzhou Medical University HospitalDepartment of Rehabilitation, Binzhou Medical University HospitalAbstract Purpose This study aimed to evaluate the protective effects of gentiopicroside against lipopolysaccharide-induced chondrocyte inflammation. Methods SW 1353 chondrosarcoma cells were stimulated with LPS (5 μg/ml) for 24 h and treated with different concentrations of gentiopicroside (GPS) for 24 h. The toxic effects of GPS on chondrocytes were determined using a CCK-8 assay and EdU staining. Western blotting, qPCR, and immunofluorescence analysis were used to examine the protective effect of GPS against the inflammatory response in chondrocytes induced by lipopolysaccharide (LPS). One-way ANOVA was used to compare the differences between the groups (significance level of 0.05). Results The CCK-8 results showed that 10, 20 and 40 μM GPS had no significant toxic effects on chondrocytes; GPS effectively reduced the production of IL-1β and PGE2, reversed LPS-induced extracellular matrix degradation in cartilage by inhibiting the Stat3/Runx2 signaling pathway, and suppressed the hypertrophic transformation of SW 1353 chondrosarcoma cells. Conclusion Our study demonstrated that GPS significantly inhibited the LPS-induced inflammatory response and hypertrophic cellular degeneration in SW 1353 chondrosarcoma cells and is a valuable traditional Chinese medicine for the treatment of knee osteoarthritis.https://doi.org/10.1186/s13018-024-04676-1OsteoarthritisKnee osteoarthritisGentiopicrosideLipopolysaccharideHypertrophy |
| spellingShingle | Longfei Li Qianqian Fan Yixuan Zhao Qian Zhang Gaofeng Qin Chen Li Wei Li Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes Journal of Orthopaedic Surgery and Research Osteoarthritis Knee osteoarthritis Gentiopicroside Lipopolysaccharide Hypertrophy |
| title | Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes |
| title_full | Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes |
| title_fullStr | Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes |
| title_full_unstemmed | Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes |
| title_short | Gentiopicroside ameliorates the lipopolysaccharide-induced inflammatory response and hypertrophy in chondrocytes |
| title_sort | gentiopicroside ameliorates the lipopolysaccharide induced inflammatory response and hypertrophy in chondrocytes |
| topic | Osteoarthritis Knee osteoarthritis Gentiopicroside Lipopolysaccharide Hypertrophy |
| url | https://doi.org/10.1186/s13018-024-04676-1 |
| work_keys_str_mv | AT longfeili gentiopicrosideamelioratesthelipopolysaccharideinducedinflammatoryresponseandhypertrophyinchondrocytes AT qianqianfan gentiopicrosideamelioratesthelipopolysaccharideinducedinflammatoryresponseandhypertrophyinchondrocytes AT yixuanzhao gentiopicrosideamelioratesthelipopolysaccharideinducedinflammatoryresponseandhypertrophyinchondrocytes AT qianzhang gentiopicrosideamelioratesthelipopolysaccharideinducedinflammatoryresponseandhypertrophyinchondrocytes AT gaofengqin gentiopicrosideamelioratesthelipopolysaccharideinducedinflammatoryresponseandhypertrophyinchondrocytes AT chenli gentiopicrosideamelioratesthelipopolysaccharideinducedinflammatoryresponseandhypertrophyinchondrocytes AT weili gentiopicrosideamelioratesthelipopolysaccharideinducedinflammatoryresponseandhypertrophyinchondrocytes |