Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia
In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot,...
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| Format: | Article |
| Language: | English |
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Sciendo
2016-08-01
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| Series: | Polish Journal of Microbiology |
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| Online Access: | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/3/pdf/10.5604_17331331.1215610.pdf |
| _version_ | 1811220757814968320 |
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| author | Badreddine Mechri Amel Medhioub Mohamed Nejib Medhioub Mahjoub Aouni |
| author_facet | Badreddine Mechri Amel Medhioub Mohamed Nejib Medhioub Mahjoub Aouni |
| author_sort | Badreddine Mechri |
| collection | DOAJ |
| description | In the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrio extracellular product were also observed among HeLa and Vero cells. |
| first_indexed | 2024-04-12T07:47:59Z |
| format | Article |
| id | doaj.art-a66399fcb0784babaac66ecc1fc57de8 |
| institution | Directory Open Access Journal |
| issn | 1733-1331 2544-4646 |
| language | English |
| last_indexed | 2024-04-12T07:47:59Z |
| publishDate | 2016-08-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Polish Journal of Microbiology |
| spelling | doaj.art-a66399fcb0784babaac66ecc1fc57de82022-12-22T03:41:40ZengSciendoPolish Journal of Microbiology1733-13312544-46462016-08-0165310.5604/17331331.1215610Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, TunisiaBadreddine Mechri0Amel Medhioub1Mohamed Nejib Medhioub2Mahjoub Aouni3Laboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, TunisiaLaboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, TunisiaLaboratory of Aquaculture, National Institute of Marine Sciences and Technology, Monastir, TunisiaLaboratory of Contagious Diseases and Biologically Active Substances, Faculty of Pharmacy, University of Monastir, Monastir, TunisiaIn the current study, 65 Vibrio spp. were isolated from the Monastir lagoon water, were characterized phenotypically and genotypically. In addition, we looked for the presence of three Vibrio parahaemolyticus virulence genes (tlh, trh and tdh) and ten Vibrio cholerae virulence genes (ctxA, vpi, zot, ace, toxR, toxT, tosS, toxRS, tcpA and cpP). We also investigated the antibiotic susceptibilities and the adherence ability of the identified strains to abiotic material and to biotic surfaces. The cytotoxicity activity against HeLa and Vero cell lines were also carried out for all tested strains. All Vibrio isolates were identified to the species level and produced several hydrolytic exoenzymes. The results also revealed that all strains were expressing high rates of resistance to tested antibiotics. The minimum inhibitory concentration (MIC) values showed that tetracycline and chloramphenicol were the most effective antibiotics against the tested bacteria. Vibrio alginolyticus and V. cholerae species were the most adhesive strains to both biotic and abiotic surfaces. Besides, V. alginolyticus isolates has the high levels of recombination of genes encoding V. cholerae and V. parahaemolyticus virulence factors. In vitro cytotoxic activities of several Vibrio extracellular product were also observed among HeLa and Vero cells.https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/3/pdf/10.5604_17331331.1215610.pdfVibrio spp.antibiotic susceptibilitybiofilmMonastir lagoonvirulence genes |
| spellingShingle | Badreddine Mechri Amel Medhioub Mohamed Nejib Medhioub Mahjoub Aouni Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia Polish Journal of Microbiology Vibrio spp. antibiotic susceptibility biofilm Monastir lagoon virulence genes |
| title | Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia |
| title_full | Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia |
| title_fullStr | Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia |
| title_full_unstemmed | Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia |
| title_short | Prevalence of Biofilm Formation and Wide Distribution of Virulence Associated Genes among Vibrio spp. Strains Isolated from the Monastir Lagoon, Tunisia |
| title_sort | prevalence of biofilm formation and wide distribution of virulence associated genes among vibrio spp strains isolated from the monastir lagoon tunisia |
| topic | Vibrio spp. antibiotic susceptibility biofilm Monastir lagoon virulence genes |
| url | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/65/3/pdf/10.5604_17331331.1215610.pdf |
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