In Vitro Research Reproducibility: Keeping Up High Standards
Concern regarding the reproducibility of observations in life science research has emerged in recent years, particularly in view of unfavorable experiences with preclinical in vivo research. The use of cell-based systems has increasingly replaced in vivo research and the application of in vitro mode...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-12-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphar.2019.01484/full |
| _version_ | 1818195291404238848 |
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| author | Cordula Hirsch Stefan Schildknecht |
| author_facet | Cordula Hirsch Stefan Schildknecht |
| author_sort | Cordula Hirsch |
| collection | DOAJ |
| description | Concern regarding the reproducibility of observations in life science research has emerged in recent years, particularly in view of unfavorable experiences with preclinical in vivo research. The use of cell-based systems has increasingly replaced in vivo research and the application of in vitro models enjoys an ever-growing popularity. To avoid repeating past mistakes, high standards of reproducibility and reliability must be established and maintained in the field of in vitro biomedical research. Detailed guidance documenting the appropriate handling of cells has been authored, but was received with quite disparate perception by different branches in biomedical research. In that regard, we intend to raise awareness of the reproducibility issue among scientists in all branches of contemporary life science research and their individual responsibility in this matter. We have herein compiled a selection of the most susceptible steps of everyday in vitro cell culture routines that have the potential to influence cell quality and recommend practices to minimize the likelihood of poor cell quality impairing reproducibility with modest investment of time and resources. |
| first_indexed | 2024-12-12T01:15:51Z |
| format | Article |
| id | doaj.art-a663b7f0d7f34a02bd901abb36098826 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-12-12T01:15:51Z |
| publishDate | 2019-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-a663b7f0d7f34a02bd901abb360988262022-12-22T00:43:21ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122019-12-011010.3389/fphar.2019.01484492837In Vitro Research Reproducibility: Keeping Up High StandardsCordula Hirsch0Stefan Schildknecht1Particles-Biology Interactions Laboratory, Swiss Federal Laboratories for Materials Science and Technology (Empa), St. Gallen, SwitzerlandIn vitro Toxicology and Biomedicine, Department of Biology, University of Konstanz, Konstanz, GermanyConcern regarding the reproducibility of observations in life science research has emerged in recent years, particularly in view of unfavorable experiences with preclinical in vivo research. The use of cell-based systems has increasingly replaced in vivo research and the application of in vitro models enjoys an ever-growing popularity. To avoid repeating past mistakes, high standards of reproducibility and reliability must be established and maintained in the field of in vitro biomedical research. Detailed guidance documenting the appropriate handling of cells has been authored, but was received with quite disparate perception by different branches in biomedical research. In that regard, we intend to raise awareness of the reproducibility issue among scientists in all branches of contemporary life science research and their individual responsibility in this matter. We have herein compiled a selection of the most susceptible steps of everyday in vitro cell culture routines that have the potential to influence cell quality and recommend practices to minimize the likelihood of poor cell quality impairing reproducibility with modest investment of time and resources.https://www.frontiersin.org/article/10.3389/fphar.2019.01484/fullcell culture modelsreproducibilitytoxicologygood cell culture practicenew approach methods (NAM) |
| spellingShingle | Cordula Hirsch Stefan Schildknecht In Vitro Research Reproducibility: Keeping Up High Standards Frontiers in Pharmacology cell culture models reproducibility toxicology good cell culture practice new approach methods (NAM) |
| title | In Vitro Research Reproducibility: Keeping Up High Standards |
| title_full | In Vitro Research Reproducibility: Keeping Up High Standards |
| title_fullStr | In Vitro Research Reproducibility: Keeping Up High Standards |
| title_full_unstemmed | In Vitro Research Reproducibility: Keeping Up High Standards |
| title_short | In Vitro Research Reproducibility: Keeping Up High Standards |
| title_sort | in vitro research reproducibility keeping up high standards |
| topic | cell culture models reproducibility toxicology good cell culture practice new approach methods (NAM) |
| url | https://www.frontiersin.org/article/10.3389/fphar.2019.01484/full |
| work_keys_str_mv | AT cordulahirsch invitroresearchreproducibilitykeepinguphighstandards AT stefanschildknecht invitroresearchreproducibilitykeepinguphighstandards |