Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy
Depth-resolved label-free optical imaging by the method of multiphoton autofluorescence microscopy (MPAM) may offer new ways to examine cellular and extracellular atypia associated with epithelial squamous cell carcinoma (SCC). MPAM was evaluated for its ability to identify cellular and microstructu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/15/4/1302 |
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| author | Paula Patricia Villarreal Rahul Pal Suimin Qiu Orly Coblens Alejandro Villasante-Tezanos Vicente Resto Susan McCammon Gracie Vargas |
| author_facet | Paula Patricia Villarreal Rahul Pal Suimin Qiu Orly Coblens Alejandro Villasante-Tezanos Vicente Resto Susan McCammon Gracie Vargas |
| author_sort | Paula Patricia Villarreal |
| collection | DOAJ |
| description | Depth-resolved label-free optical imaging by the method of multiphoton autofluorescence microscopy (MPAM) may offer new ways to examine cellular and extracellular atypia associated with epithelial squamous cell carcinoma (SCC). MPAM was evaluated for its ability to identify cellular and microstructural atypia in head and neck tissues from resected discarded tumor tissue. Three-dimensional image volumes were obtained from tissues from the floor of the mouth, tongue, and larynx, and were then processed for histology. MPAM micrographs were evaluated for qualitative metrics of cell atypia and quantitative measures associated with nuclear pleomorphism. Statistical analyses correlated MPAM endpoints with histological grade from each imaged site. Cellular overcrowding, discohesion, anisonucleosis, and multinucleated cells, as observed through MPAM, were found to be statistically associated with dysplasia and SCC grading, but not in histologically benign regions. A quantitative measure of the coefficient of variance in nuclear size in SCC and dysplasia was statistically elevated above histologically benign regions. MPAM also allowed for the identification of cellular heterogeneity across transitional areas and other features, such as inflammatory infiltrates. In the future, MPAM could be evaluated for the non-invasive detection of neoplasia, possibly as an adjunct to traditional conventional examination and biopsy. |
| first_indexed | 2024-03-11T09:02:11Z |
| format | Article |
| id | doaj.art-a666bf5d2b154274b0065cc0ae5ebb13 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T09:02:11Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-a666bf5d2b154274b0065cc0ae5ebb132023-11-16T19:39:09ZengMDPI AGCancers2072-66942023-02-01154130210.3390/cancers15041302Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence MicroscopyPaula Patricia Villarreal0Rahul Pal1Suimin Qiu2Orly Coblens3Alejandro Villasante-Tezanos4Vicente Resto5Susan McCammon6Gracie Vargas7The Institute for Translational Sciences, University of Texas Medical Branch, Galveston, TX 77555, USAAthinoula A. Martinos Center for Biomedical Imaging, Massachusetts General Hospital, Charlestown, MA 02129, USADepartment of Pathology, Division of Surgical Pathology, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Otolaryngology, Head & Neck Surgery, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Biostatistics and Data Science, School for Public and Population Health, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Otolaryngology, Head & Neck Surgery, University of Texas Medical Branch, Galveston, TX 77555, USADepartment of Otolaryngology, Head & Neck Surgery Oncology Division, The University of Alabama at Birmingham, Birmingham, AL 35294, USADepartment of Neurobiology, University of Texas Medical Branch, Galveston, TX 77555, USADepth-resolved label-free optical imaging by the method of multiphoton autofluorescence microscopy (MPAM) may offer new ways to examine cellular and extracellular atypia associated with epithelial squamous cell carcinoma (SCC). MPAM was evaluated for its ability to identify cellular and microstructural atypia in head and neck tissues from resected discarded tumor tissue. Three-dimensional image volumes were obtained from tissues from the floor of the mouth, tongue, and larynx, and were then processed for histology. MPAM micrographs were evaluated for qualitative metrics of cell atypia and quantitative measures associated with nuclear pleomorphism. Statistical analyses correlated MPAM endpoints with histological grade from each imaged site. Cellular overcrowding, discohesion, anisonucleosis, and multinucleated cells, as observed through MPAM, were found to be statistically associated with dysplasia and SCC grading, but not in histologically benign regions. A quantitative measure of the coefficient of variance in nuclear size in SCC and dysplasia was statistically elevated above histologically benign regions. MPAM also allowed for the identification of cellular heterogeneity across transitional areas and other features, such as inflammatory infiltrates. In the future, MPAM could be evaluated for the non-invasive detection of neoplasia, possibly as an adjunct to traditional conventional examination and biopsy.https://www.mdpi.com/2072-6694/15/4/1302multiphoton microscopyautofluorescencesecond harmonic generationsquamous cell carcinomahead and neckepithelium |
| spellingShingle | Paula Patricia Villarreal Rahul Pal Suimin Qiu Orly Coblens Alejandro Villasante-Tezanos Vicente Resto Susan McCammon Gracie Vargas Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy Cancers multiphoton microscopy autofluorescence second harmonic generation squamous cell carcinoma head and neck epithelium |
| title | Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy |
| title_full | Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy |
| title_fullStr | Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy |
| title_full_unstemmed | Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy |
| title_short | Label-Free Imaging and Histo-Optical Evaluation of Head and Neck Cancers with Multiphoton Autofluorescence Microscopy |
| title_sort | label free imaging and histo optical evaluation of head and neck cancers with multiphoton autofluorescence microscopy |
| topic | multiphoton microscopy autofluorescence second harmonic generation squamous cell carcinoma head and neck epithelium |
| url | https://www.mdpi.com/2072-6694/15/4/1302 |
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