A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i>
<i>Limnospira maxima</i> is a remarkable organism showing great potential as a versatile and sustainable food source, offering a powerful solution to address the pressing issues of malnutrition and undernourishment worldwide. <i>L. maxima</i> contains high amounts of proteins...
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MDPI AG
2023-07-01
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| Series: | Methods and Protocols |
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| Online Access: | https://www.mdpi.com/2409-9279/6/4/62 |
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| author | Yirlis Yadeth Pineda-Rodriguez Marcelo F. Pompelli Alfredo Jarma-Orozco Novisel Veitía Rodríguez Luis Alfonso Rodriguez-Paez |
| author_facet | Yirlis Yadeth Pineda-Rodriguez Marcelo F. Pompelli Alfredo Jarma-Orozco Novisel Veitía Rodríguez Luis Alfonso Rodriguez-Paez |
| author_sort | Yirlis Yadeth Pineda-Rodriguez |
| collection | DOAJ |
| description | <i>Limnospira maxima</i> is a remarkable organism showing great potential as a versatile and sustainable food source, offering a powerful solution to address the pressing issues of malnutrition and undernourishment worldwide. <i>L. maxima</i> contains high amounts of proteins, vitamins, minerals, and essential fatty acids. It can be grown in both bioreactors and open systems; however, before considering industrial production, optimization studies of the cultivation must be conducted to obtain knowledge about the ideal environmental conditions. Additionally, for the molecular typing of <i>L. maxima</i> strains and their industrial scaling, high-quality and large quantity DNA extraction is required. Notwithstanding, DNA extraction from <i>L. maxima</i> can be challenging due to the low amount of DNA in cells and the presence of difficult-to-remove substances such as polysaccharides and polyphenols. In this study, the quality and quantity of DNA extracted from two types of <i>L. maxima</i> samples (<i>Limnospira maxima</i> strain SISCA accession GenBank: OR195505.1) were evaluated using three commercially available DNA extraction kits and two types of input biological material. The results showed that Pbact-P kit had the highest quantity and quality of DNA, while CTAB-P allowed for a higher quantity and quality of RNA, making them optimal protocols for nucleic acid extraction to improve PCR, rt-PCR, and genome sequencing of <i>L. maxima</i> compared with other extraction methods. |
| first_indexed | 2024-03-10T23:41:41Z |
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| id | doaj.art-a6684c04bad747eeaeb0d3cc41d60458 |
| institution | Directory Open Access Journal |
| issn | 2409-9279 |
| language | English |
| last_indexed | 2024-03-10T23:41:41Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Methods and Protocols |
| spelling | doaj.art-a6684c04bad747eeaeb0d3cc41d604582023-11-19T02:26:12ZengMDPI AGMethods and Protocols2409-92792023-07-01646210.3390/mps6040062A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i>Yirlis Yadeth Pineda-Rodriguez0Marcelo F. Pompelli1Alfredo Jarma-Orozco2Novisel Veitía Rodríguez3Luis Alfonso Rodriguez-Paez4Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, ColombiaLaboratorio de Biología Molecular Aplicada (INVEPAR) de la Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, ColombiaLaboratorio de Biología Molecular Aplicada (INVEPAR) de la Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, ColombiaInstituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Santa Clara 54830, CubaLaboratorio de Biología Molecular Aplicada (INVEPAR) de la Facultad de Ciencias Agrícolas, Universidad de Córdoba, Montería 230002, Colombia<i>Limnospira maxima</i> is a remarkable organism showing great potential as a versatile and sustainable food source, offering a powerful solution to address the pressing issues of malnutrition and undernourishment worldwide. <i>L. maxima</i> contains high amounts of proteins, vitamins, minerals, and essential fatty acids. It can be grown in both bioreactors and open systems; however, before considering industrial production, optimization studies of the cultivation must be conducted to obtain knowledge about the ideal environmental conditions. Additionally, for the molecular typing of <i>L. maxima</i> strains and their industrial scaling, high-quality and large quantity DNA extraction is required. Notwithstanding, DNA extraction from <i>L. maxima</i> can be challenging due to the low amount of DNA in cells and the presence of difficult-to-remove substances such as polysaccharides and polyphenols. In this study, the quality and quantity of DNA extracted from two types of <i>L. maxima</i> samples (<i>Limnospira maxima</i> strain SISCA accession GenBank: OR195505.1) were evaluated using three commercially available DNA extraction kits and two types of input biological material. The results showed that Pbact-P kit had the highest quantity and quality of DNA, while CTAB-P allowed for a higher quantity and quality of RNA, making them optimal protocols for nucleic acid extraction to improve PCR, rt-PCR, and genome sequencing of <i>L. maxima</i> compared with other extraction methods.https://www.mdpi.com/2409-9279/6/4/62DNA extractionA260/A280PCR<i>Arthrospira maxima</i> |
| spellingShingle | Yirlis Yadeth Pineda-Rodriguez Marcelo F. Pompelli Alfredo Jarma-Orozco Novisel Veitía Rodríguez Luis Alfonso Rodriguez-Paez A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i> Methods and Protocols DNA extraction A260/A280 PCR <i>Arthrospira maxima</i> |
| title | A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i> |
| title_full | A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i> |
| title_fullStr | A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i> |
| title_full_unstemmed | A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i> |
| title_short | A New and Profitable Protocol to DNA Extraction in <i>Limnospira maxima</i> |
| title_sort | new and profitable protocol to dna extraction in i limnospira maxima i |
| topic | DNA extraction A260/A280 PCR <i>Arthrospira maxima</i> |
| url | https://www.mdpi.com/2409-9279/6/4/62 |
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