Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro Study
Introduction: Oral cavity contains a plethora of microorganisms. These microorganisms have shown both beneficence and maleficence. The most common disease affecting the oral cavity not only to the human being but also for the animals was dental caries that are proved to have multi-bacterial origin....
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Journal of Orofacial Sciences |
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| Online Access: | http://www.jofs.in/article.asp?issn=0975-8844;year=2020;volume=12;issue=2;spage=96;epage=100;aulast=Magesh |
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| author | K T Magesh R Aravindhan M Sathya Kumar A Sivachandran |
| author_facet | K T Magesh R Aravindhan M Sathya Kumar A Sivachandran |
| author_sort | K T Magesh |
| collection | DOAJ |
| description | Introduction: Oral cavity contains a plethora of microorganisms. These microorganisms have shown both beneficence and maleficence. The most common disease affecting the oral cavity not only to the human being but also for the animals was dental caries that are proved to have multi-bacterial origin. Bacterial species such as Streptococcus mutans (S.mutans), S.sanguis and Lactobacillus fermentum plays a major role in the pathogenesis of this disease. In this study, the crude extract of sargassum wightii is tested for its antibacterial efficacy against the S.mutants. Materials and Methods: The brown seaweed S. wightii was collected from the Gulf of Mannar. The samples were cut into small pieces and shade dried for five days. After drying, the samples were grounded and stored. Stock solution was prepared using soxhlet apparatus. The organic extract of the seaweed S. wightii was assayed for the antimicrobial activity against S. mutans using agar diffusion method and the result was compared with the positive control sample (Ampicillin). Results: Stock solution of S.wightii used in this study was prepared in the following grades 500, 750, and 1000 μg. It was found that zone of inhibition increased steadily as the concentration of the extract increased. Highest inhibition zone of about 23 mm was found out at the concentration of 1000 μg, whereas the control showed 38 mm. Result showed the definite antibacterial activity of S.wightii even with the crude extract. Conclusion: Majority of the treatment and preventive approaches for dental caries are based on the use of synthetic antibiotics and chemicals. Their excessive use leads to alterations in the oral and intestinal microbiome, development of resistance and other undesirable side effects. The use of natural resources or products has proven to be copious source of biologically active compounds. |
| first_indexed | 2024-12-14T21:51:33Z |
| format | Article |
| id | doaj.art-a6692e999c394e12a5bf927f21423540 |
| institution | Directory Open Access Journal |
| issn | 0975-8844 |
| language | English |
| last_indexed | 2024-12-14T21:51:33Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Orofacial Sciences |
| spelling | doaj.art-a6692e999c394e12a5bf927f214235402022-12-21T22:46:14ZengWolters Kluwer Medknow PublicationsJournal of Orofacial Sciences0975-88442020-01-011229610010.4103/jofs.jofs_80_20Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro StudyK T MageshR AravindhanM Sathya KumarA SivachandranIntroduction: Oral cavity contains a plethora of microorganisms. These microorganisms have shown both beneficence and maleficence. The most common disease affecting the oral cavity not only to the human being but also for the animals was dental caries that are proved to have multi-bacterial origin. Bacterial species such as Streptococcus mutans (S.mutans), S.sanguis and Lactobacillus fermentum plays a major role in the pathogenesis of this disease. In this study, the crude extract of sargassum wightii is tested for its antibacterial efficacy against the S.mutants. Materials and Methods: The brown seaweed S. wightii was collected from the Gulf of Mannar. The samples were cut into small pieces and shade dried for five days. After drying, the samples were grounded and stored. Stock solution was prepared using soxhlet apparatus. The organic extract of the seaweed S. wightii was assayed for the antimicrobial activity against S. mutans using agar diffusion method and the result was compared with the positive control sample (Ampicillin). Results: Stock solution of S.wightii used in this study was prepared in the following grades 500, 750, and 1000 μg. It was found that zone of inhibition increased steadily as the concentration of the extract increased. Highest inhibition zone of about 23 mm was found out at the concentration of 1000 μg, whereas the control showed 38 mm. Result showed the definite antibacterial activity of S.wightii even with the crude extract. Conclusion: Majority of the treatment and preventive approaches for dental caries are based on the use of synthetic antibiotics and chemicals. Their excessive use leads to alterations in the oral and intestinal microbiome, development of resistance and other undesirable side effects. The use of natural resources or products has proven to be copious source of biologically active compounds.http://www.jofs.in/article.asp?issn=0975-8844;year=2020;volume=12;issue=2;spage=96;epage=100;aulast=Mageshantibacterialalgaedental cariesseaweeds. wightiitooth decay |
| spellingShingle | K T Magesh R Aravindhan M Sathya Kumar A Sivachandran Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro Study Journal of Orofacial Sciences antibacterial algae dental caries seaweed s. wightii tooth decay |
| title | Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro Study |
| title_full | Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro Study |
| title_fullStr | Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro Study |
| title_full_unstemmed | Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro Study |
| title_short | Antibacterial Efficacy of the Extract of Sargassum Wightii Against Oral Pathogen − An In Vitro Study |
| title_sort | antibacterial efficacy of the extract of sargassum wightii against oral pathogen an in vitro study |
| topic | antibacterial algae dental caries seaweed s. wightii tooth decay |
| url | http://www.jofs.in/article.asp?issn=0975-8844;year=2020;volume=12;issue=2;spage=96;epage=100;aulast=Magesh |
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