Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer
Cryopreservation is the final step of stem cell production before the cryostorage of the product. Conventional methods of adding cryoprotecting agents (CPA) into the cells can be manual or automated with robotic arms. However, challenging issues with these methods at industrial-scale production are...
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| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/13/9/1516 |
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| author | Lin Ding Sajad Razavi Bazaz Jesus Shrestha Hoseyn A. Amiri Sima Mas-hafi Balarka Banerjee Graham Vesey Morteza Miansari Majid Ebrahimi Warkiani |
| author_facet | Lin Ding Sajad Razavi Bazaz Jesus Shrestha Hoseyn A. Amiri Sima Mas-hafi Balarka Banerjee Graham Vesey Morteza Miansari Majid Ebrahimi Warkiani |
| author_sort | Lin Ding |
| collection | DOAJ |
| description | Cryopreservation is the final step of stem cell production before the cryostorage of the product. Conventional methods of adding cryoprotecting agents (CPA) into the cells can be manual or automated with robotic arms. However, challenging issues with these methods at industrial-scale production are the insufficient mixing of cells and CPA, leading to damage of cells, discontinuous feeding, the batch-to-batch difference in products, and, occasionally, cross-contamination. Therefore, the current study proposes an alternative way to overcome the abovementioned challenges; a highly efficient micromixer for low-cost, continuous, labour-free, and automated mixing of stem cells with CPA solutions. Our results show that our micromixer provides a more homogenous mixing of cells and CPA compared to the manual mixing method, while the cell properties, including surface markers, differentiation potential, proliferation, morphology, and therapeutic potential, are well preserved. |
| first_indexed | 2024-03-09T23:08:06Z |
| format | Article |
| id | doaj.art-a669c23d8f0e4c1f9bdebbcd2d1def54 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-09T23:08:06Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-a669c23d8f0e4c1f9bdebbcd2d1def542023-11-23T17:50:49ZengMDPI AGMicromachines2072-666X2022-09-01139151610.3390/mi13091516Rapid and Continuous Cryopreservation of Stem Cells with a 3D MicromixerLin Ding0Sajad Razavi Bazaz1Jesus Shrestha2Hoseyn A. Amiri3Sima Mas-hafi4Balarka Banerjee5Graham Vesey6Morteza Miansari7Majid Ebrahimi Warkiani8School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaMicro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, IranMicro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, IranRegeneus Ltd., Paddington, Sydney, NSW 2021, AustraliaRegeneus Ltd., Paddington, Sydney, NSW 2021, AustraliaMicro+Nanosystems & Applied Biophysics Laboratory, Department of Mechanical Engineering, Babol Noshirvani University of Technology, P.O. Box 484, Babol 47148-71167, IranSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaCryopreservation is the final step of stem cell production before the cryostorage of the product. Conventional methods of adding cryoprotecting agents (CPA) into the cells can be manual or automated with robotic arms. However, challenging issues with these methods at industrial-scale production are the insufficient mixing of cells and CPA, leading to damage of cells, discontinuous feeding, the batch-to-batch difference in products, and, occasionally, cross-contamination. Therefore, the current study proposes an alternative way to overcome the abovementioned challenges; a highly efficient micromixer for low-cost, continuous, labour-free, and automated mixing of stem cells with CPA solutions. Our results show that our micromixer provides a more homogenous mixing of cells and CPA compared to the manual mixing method, while the cell properties, including surface markers, differentiation potential, proliferation, morphology, and therapeutic potential, are well preserved.https://www.mdpi.com/2072-666X/13/9/1516cryopreservation3D printingmicrofluidicsstem cellsbioprocessing industry |
| spellingShingle | Lin Ding Sajad Razavi Bazaz Jesus Shrestha Hoseyn A. Amiri Sima Mas-hafi Balarka Banerjee Graham Vesey Morteza Miansari Majid Ebrahimi Warkiani Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer Micromachines cryopreservation 3D printing microfluidics stem cells bioprocessing industry |
| title | Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer |
| title_full | Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer |
| title_fullStr | Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer |
| title_full_unstemmed | Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer |
| title_short | Rapid and Continuous Cryopreservation of Stem Cells with a 3D Micromixer |
| title_sort | rapid and continuous cryopreservation of stem cells with a 3d micromixer |
| topic | cryopreservation 3D printing microfluidics stem cells bioprocessing industry |
| url | https://www.mdpi.com/2072-666X/13/9/1516 |
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