Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis
<i>Aspergillus niger</i> is one of the most important sources of secondary metabolites (SMs), with a wide array of pharmacological effects, including anti-inflammatory, antitumor, immunomodulatory and antioxidant effects. However, the biosynthetic analysis of these bioactive components h...
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MDPI AG
2022-05-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/6/591 |
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| author | Ping Wang Shuang Xu Yuqi Tang Hong Wang Xuelian Bai Huawei Zhang |
| author_facet | Ping Wang Shuang Xu Yuqi Tang Hong Wang Xuelian Bai Huawei Zhang |
| author_sort | Ping Wang |
| collection | DOAJ |
| description | <i>Aspergillus niger</i> is one of the most important sources of secondary metabolites (SMs), with a wide array of pharmacological effects, including anti-inflammatory, antitumor, immunomodulatory and antioxidant effects. However, the biosynthetic analysis of these bioactive components has been rarely reported owing to the lack of high-quality genome sequences and comprehensive analysis. In this study, the whole genome of one marine-sponge-derived strain <i>A. niger</i> L14 was sequenced and assembled as well as in-depth bioinformatic analysis. The results indicated that the sequence assembly of strain L14 generated one high-quality genome with a total size of 36.1 Mb, a G + C content of 45.3% and an N50 scaffold of 4.2 Mb. Gene annotation was extensively deployed using various BLAST databases, including non-redudant (Nr) protein sequence, nucleotide (Nt) sequence, Swiss-Prot, Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) as well as Pathogen Host Interactions (PHI) and Carbohydrate-active enzymes (CAZy) databases. AntiSMASH analysis revealed that this marine strain harbors a total of 69 SMs biosynthesis gene clusters (BGCs), including 17 PKSs, 18 NRPSs, 21 NRPS-likes, 9 terpenes, 2 indoles, 1 betalactone and 1 siderophore, suggesting its biosynthetic potential to produce a wide variety of SMs. These findings will assist in future investigations on the genetic basis of strain L14 and provide insights into its new bioactive SMs for new drug discovery. |
| first_indexed | 2024-03-09T23:22:42Z |
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| last_indexed | 2024-03-09T23:22:42Z |
| publishDate | 2022-05-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-a669fefc2b964a38902774485cad5d7c2023-11-23T17:24:38ZengMDPI AGJournal of Fungi2309-608X2022-05-018659110.3390/jof8060591Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites BiosynthesisPing Wang0Shuang Xu1Yuqi Tang2Hong Wang3Xuelian Bai4Huawei Zhang5School of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, ChinaSchool of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, ChinaSchool of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, ChinaSchool of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, ChinaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, ChinaSchool of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, China<i>Aspergillus niger</i> is one of the most important sources of secondary metabolites (SMs), with a wide array of pharmacological effects, including anti-inflammatory, antitumor, immunomodulatory and antioxidant effects. However, the biosynthetic analysis of these bioactive components has been rarely reported owing to the lack of high-quality genome sequences and comprehensive analysis. In this study, the whole genome of one marine-sponge-derived strain <i>A. niger</i> L14 was sequenced and assembled as well as in-depth bioinformatic analysis. The results indicated that the sequence assembly of strain L14 generated one high-quality genome with a total size of 36.1 Mb, a G + C content of 45.3% and an N50 scaffold of 4.2 Mb. Gene annotation was extensively deployed using various BLAST databases, including non-redudant (Nr) protein sequence, nucleotide (Nt) sequence, Swiss-Prot, Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Clusters of Orthologous Groups (COG) as well as Pathogen Host Interactions (PHI) and Carbohydrate-active enzymes (CAZy) databases. AntiSMASH analysis revealed that this marine strain harbors a total of 69 SMs biosynthesis gene clusters (BGCs), including 17 PKSs, 18 NRPSs, 21 NRPS-likes, 9 terpenes, 2 indoles, 1 betalactone and 1 siderophore, suggesting its biosynthetic potential to produce a wide variety of SMs. These findings will assist in future investigations on the genetic basis of strain L14 and provide insights into its new bioactive SMs for new drug discovery.https://www.mdpi.com/2309-608X/8/6/591<i>Aspergillus niger</i>symbiotic microorganismgenome sequencingantiSMASHnatural productbiosynthetic potential |
| spellingShingle | Ping Wang Shuang Xu Yuqi Tang Hong Wang Xuelian Bai Huawei Zhang Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis Journal of Fungi <i>Aspergillus niger</i> symbiotic microorganism genome sequencing antiSMASH natural product biosynthetic potential |
| title | Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis |
| title_full | Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis |
| title_fullStr | Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis |
| title_full_unstemmed | Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis |
| title_short | Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain <i>Aspergillus niger</i> L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis |
| title_sort | genomic and antismash analyses of marine sponge derived strain i aspergillus niger i l14 unveiling its vast potential of secondary metabolites biosynthesis |
| topic | <i>Aspergillus niger</i> symbiotic microorganism genome sequencing antiSMASH natural product biosynthetic potential |
| url | https://www.mdpi.com/2309-608X/8/6/591 |
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