Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure
Fluoxetine (FLX) is an antidepressant that is increasingly being detected in aquatic environments. However, this contaminated FLX can affect aquatic organisms. Therefore, the aim of this study was to evaluate the genotoxic, mutagenic, and cytotoxic potential of FLX on erythrocytes in Nile tilapia (O...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-01-01
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| Series: | Toxicology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214750022000610 |
| _version_ | 1811178649850740736 |
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| author | Pachara Vijitkul Mesayamas Kongsema Thularath Toommakorn Vasakorn Bullangpoti |
| author_facet | Pachara Vijitkul Mesayamas Kongsema Thularath Toommakorn Vasakorn Bullangpoti |
| author_sort | Pachara Vijitkul |
| collection | DOAJ |
| description | Fluoxetine (FLX) is an antidepressant that is increasingly being detected in aquatic environments. However, this contaminated FLX can affect aquatic organisms. Therefore, the aim of this study was to evaluate the genotoxic, mutagenic, and cytotoxic potential of FLX on erythrocytes in Nile tilapia (Oreochromis niloticus) after acute exposure. Fish were exposed to different concentrations of FLX (10, 100 and 1000 µg/L) for 96 h. Then, the condition factor (K value) was used to assess the general fish condition. The genotoxicity was investigated using a comet assay, and the mutagenicity was examined using micronucleus (MN) and erythrocytic nuclear abnormalities (ENAs) assays. In addition, the cytotoxicity was analyzed by erythrocyte morphometry and erythrocyte maturity index (EMI). The results showed that FLX did not affect the fish’s health. Nevertheless, 100 and 1000 µg/L FLX significantly increased DNA damage. Furthermore, a higher concentration of FLX presented a significantly increased frequency of MNs and ENAs, also leading to changes in some erythrocyte morphometric indices and significantly decreased mature erythrocytes. In conclusion, our results indicate that FLX induces genotoxic, mutagenic, and cytotoxic effects in erythrocytes of O. niloticus. |
| first_indexed | 2024-04-11T06:23:13Z |
| format | Article |
| id | doaj.art-a66c517e28ae4d06b518162d537ffca2 |
| institution | Directory Open Access Journal |
| issn | 2214-7500 |
| language | English |
| last_indexed | 2024-04-11T06:23:13Z |
| publishDate | 2022-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicology Reports |
| spelling | doaj.art-a66c517e28ae4d06b518162d537ffca22022-12-22T04:40:30ZengElsevierToxicology Reports2214-75002022-01-019588596Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposurePachara Vijitkul0Mesayamas Kongsema1Thularath Toommakorn2Vasakorn Bullangpoti3Animal Toxicology and Specialty Research Unit, Department of Zoology, Faculty of Science, Kasetsart University, Phahonyothin Road, Bangkok 10900, ThailandCorresponding authors.; Animal Toxicology and Specialty Research Unit, Department of Zoology, Faculty of Science, Kasetsart University, Phahonyothin Road, Bangkok 10900, ThailandAnimal Toxicology and Specialty Research Unit, Department of Zoology, Faculty of Science, Kasetsart University, Phahonyothin Road, Bangkok 10900, ThailandCorresponding authors.; Animal Toxicology and Specialty Research Unit, Department of Zoology, Faculty of Science, Kasetsart University, Phahonyothin Road, Bangkok 10900, ThailandFluoxetine (FLX) is an antidepressant that is increasingly being detected in aquatic environments. However, this contaminated FLX can affect aquatic organisms. Therefore, the aim of this study was to evaluate the genotoxic, mutagenic, and cytotoxic potential of FLX on erythrocytes in Nile tilapia (Oreochromis niloticus) after acute exposure. Fish were exposed to different concentrations of FLX (10, 100 and 1000 µg/L) for 96 h. Then, the condition factor (K value) was used to assess the general fish condition. The genotoxicity was investigated using a comet assay, and the mutagenicity was examined using micronucleus (MN) and erythrocytic nuclear abnormalities (ENAs) assays. In addition, the cytotoxicity was analyzed by erythrocyte morphometry and erythrocyte maturity index (EMI). The results showed that FLX did not affect the fish’s health. Nevertheless, 100 and 1000 µg/L FLX significantly increased DNA damage. Furthermore, a higher concentration of FLX presented a significantly increased frequency of MNs and ENAs, also leading to changes in some erythrocyte morphometric indices and significantly decreased mature erythrocytes. In conclusion, our results indicate that FLX induces genotoxic, mutagenic, and cytotoxic effects in erythrocytes of O. niloticus.http://www.sciencedirect.com/science/article/pii/S2214750022000610FluoxetineGenotoxicityMutagenicityCytotoxicityErythrocyteOreochromis niloticus |
| spellingShingle | Pachara Vijitkul Mesayamas Kongsema Thularath Toommakorn Vasakorn Bullangpoti Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure Toxicology Reports Fluoxetine Genotoxicity Mutagenicity Cytotoxicity Erythrocyte Oreochromis niloticus |
| title | Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure |
| title_full | Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure |
| title_fullStr | Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure |
| title_full_unstemmed | Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure |
| title_short | Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure |
| title_sort | investigation of genotoxicity mutagenicity and cytotoxicity in erythrocytes of nile tilapia oreochromis niloticus after fluoxetine exposure |
| topic | Fluoxetine Genotoxicity Mutagenicity Cytotoxicity Erythrocyte Oreochromis niloticus |
| url | http://www.sciencedirect.com/science/article/pii/S2214750022000610 |
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