Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents
Rue (R. graveolens) has been an extensively studied medicinal plant due to its rich phytochemicals content, such as furanocoumarins and flavonoids. The aim of this study was to determine the effects of varying extraction conditions on the total phenolic content, the antioxidant and antibacterial pro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-03-01
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| Series: | Plants |
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| Online Access: | http://www.mdpi.com/2223-7747/8/3/69 |
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| author | Valentina Pavić Dora Flačer Martina Jakovljević Maja Molnar Stela Jokić |
| author_facet | Valentina Pavić Dora Flačer Martina Jakovljević Maja Molnar Stela Jokić |
| author_sort | Valentina Pavić |
| collection | DOAJ |
| description | Rue (R. graveolens) has been an extensively studied medicinal plant due to its rich phytochemicals content, such as furanocoumarins and flavonoids. The aim of this study was to determine the effects of varying extraction conditions on the total phenolic content, the antioxidant and antibacterial property of rue leaves crude extracts using deep eutectic solvents with different water content. These extraction conditions include the temperature and the extraction time. The extract obtained at 30 °C, with 20% water and at 90 min, with 13.3 µg mL−1 concentration, was found to possess the highest total phenolic content (38.24 ± 0.11 mg of GAE g−1 of DM) and the highest antioxidant activity (72.53 ± 0.31%). In this study, the same extract showed the best antibacterial efficiency against all the tested strains, especially gram-negative P. aeruginosa. |
| first_indexed | 2024-12-10T17:47:00Z |
| format | Article |
| id | doaj.art-a66d193022a04e4f993032fcc5ae494e |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-10T17:47:00Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-a66d193022a04e4f993032fcc5ae494e2022-12-22T01:39:12ZengMDPI AGPlants2223-77472019-03-01836910.3390/plants8030069plants8030069Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic SolventsValentina Pavić0Dora Flačer1Martina Jakovljević2Maja Molnar3Stela Jokić4Department of Biology, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaDepartment of Biology, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8/A, 31000 Osijek, CroatiaFaculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, CroatiaFaculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, CroatiaFaculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, 31000 Osijek, CroatiaRue (R. graveolens) has been an extensively studied medicinal plant due to its rich phytochemicals content, such as furanocoumarins and flavonoids. The aim of this study was to determine the effects of varying extraction conditions on the total phenolic content, the antioxidant and antibacterial property of rue leaves crude extracts using deep eutectic solvents with different water content. These extraction conditions include the temperature and the extraction time. The extract obtained at 30 °C, with 20% water and at 90 min, with 13.3 µg mL−1 concentration, was found to possess the highest total phenolic content (38.24 ± 0.11 mg of GAE g−1 of DM) and the highest antioxidant activity (72.53 ± 0.31%). In this study, the same extract showed the best antibacterial efficiency against all the tested strains, especially gram-negative P. aeruginosa.http://www.mdpi.com/2223-7747/8/3/69Ruta graveolensnatural deep eutectic solventsextractionantioxidant activityantibacterial activity |
| spellingShingle | Valentina Pavić Dora Flačer Martina Jakovljević Maja Molnar Stela Jokić Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents Plants Ruta graveolens natural deep eutectic solvents extraction antioxidant activity antibacterial activity |
| title | Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents |
| title_full | Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents |
| title_fullStr | Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents |
| title_full_unstemmed | Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents |
| title_short | Assessment of Total Phenolic Content, In Vitro Antioxidant and Antibacterial Activity of Ruta graveolens L. Extracts Obtained by Choline Chloride Based Natural Deep Eutectic Solvents |
| title_sort | assessment of total phenolic content in vitro antioxidant and antibacterial activity of ruta graveolens l extracts obtained by choline chloride based natural deep eutectic solvents |
| topic | Ruta graveolens natural deep eutectic solvents extraction antioxidant activity antibacterial activity |
| url | http://www.mdpi.com/2223-7747/8/3/69 |
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