Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell Epitopes
The rabbit hemorrhagic disease virus (RHDV) vaccine platform is a nanoparticle composed of 180 copies of the viral capsid protein, VP60, self-assembled into virus-like particles (VLPs). RHDV VLPs are able to accept the simultaneous incorporation of target epitopes at different insertion sites. The r...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/10/2/229 |
| _version_ | 1827652384057196544 |
|---|---|
| author | María Zamora-Ceballos Noelia Moreno David Gil-Cantero José R. Castón Esther Blanco Juan Bárcena |
| author_facet | María Zamora-Ceballos Noelia Moreno David Gil-Cantero José R. Castón Esther Blanco Juan Bárcena |
| author_sort | María Zamora-Ceballos |
| collection | DOAJ |
| description | The rabbit hemorrhagic disease virus (RHDV) vaccine platform is a nanoparticle composed of 180 copies of the viral capsid protein, VP60, self-assembled into virus-like particles (VLPs). RHDV VLPs are able to accept the simultaneous incorporation of target epitopes at different insertion sites. The resulting chimeric RHDV VLPs displaying immunogenic foreign antigens have been shown to induce specific protective immune responses against inserted heterologous T-cytotoxic and B-cell epitopes in the mouse and pig models. In this study, we explored whether RHDV-based engineered VLPs can be developed as efficient multivalent vaccines co-delivering different foreign B-cell antigens. We generated bivalent chimeric RHDV VLPs displaying two model B-cell epitopes at different surface-exposed insertion sites, as well as the corresponding monovalent chimeric VLPs. The immunogenic potential of the bivalent chimeric VLPs versus the monovalent constructs was assessed in the mouse model. We found that the bivalent chimeric VLPs elicited a strong and balanced antibody response towards the two target epitopes tested, although slight reductions were observed in the levels of specific serum antibody titers induced by bivalent chimeric VLPs as compared with the corresponding monovalent constructs. These results suggest that RHDV VLPs could represent a promising platform for the development of efficient multivalent vaccines. |
| first_indexed | 2024-03-09T20:54:43Z |
| format | Article |
| id | doaj.art-a66ddbaf98754c0cb8ae28eb7a30cc4e |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-09T20:54:43Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-a66ddbaf98754c0cb8ae28eb7a30cc4e2023-11-23T22:25:37ZengMDPI AGVaccines2076-393X2022-02-0110222910.3390/vaccines10020229Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell EpitopesMaría Zamora-Ceballos0Noelia Moreno1David Gil-Cantero2José R. Castón3Esther Blanco4Juan Bárcena5Instituto Centro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, SpainInstituto Centro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, SpainDepartment of Structure of Macromolecules, Centro Nacional de Biotecnología/CSIC, Cantoblanco, 28049 Madrid, SpainDepartment of Structure of Macromolecules, Centro Nacional de Biotecnología/CSIC, Cantoblanco, 28049 Madrid, SpainInstituto Centro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, SpainInstituto Centro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, SpainThe rabbit hemorrhagic disease virus (RHDV) vaccine platform is a nanoparticle composed of 180 copies of the viral capsid protein, VP60, self-assembled into virus-like particles (VLPs). RHDV VLPs are able to accept the simultaneous incorporation of target epitopes at different insertion sites. The resulting chimeric RHDV VLPs displaying immunogenic foreign antigens have been shown to induce specific protective immune responses against inserted heterologous T-cytotoxic and B-cell epitopes in the mouse and pig models. In this study, we explored whether RHDV-based engineered VLPs can be developed as efficient multivalent vaccines co-delivering different foreign B-cell antigens. We generated bivalent chimeric RHDV VLPs displaying two model B-cell epitopes at different surface-exposed insertion sites, as well as the corresponding monovalent chimeric VLPs. The immunogenic potential of the bivalent chimeric VLPs versus the monovalent constructs was assessed in the mouse model. We found that the bivalent chimeric VLPs elicited a strong and balanced antibody response towards the two target epitopes tested, although slight reductions were observed in the levels of specific serum antibody titers induced by bivalent chimeric VLPs as compared with the corresponding monovalent constructs. These results suggest that RHDV VLPs could represent a promising platform for the development of efficient multivalent vaccines.https://www.mdpi.com/2076-393X/10/2/229virus-like particles (VLPs)chimeric VLPsnanoparticlesvaccine platformmultivalent vaccineRHDV |
| spellingShingle | María Zamora-Ceballos Noelia Moreno David Gil-Cantero José R. Castón Esther Blanco Juan Bárcena Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell Epitopes Vaccines virus-like particles (VLPs) chimeric VLPs nanoparticles vaccine platform multivalent vaccine RHDV |
| title | Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell Epitopes |
| title_full | Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell Epitopes |
| title_fullStr | Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell Epitopes |
| title_full_unstemmed | Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell Epitopes |
| title_short | Immunogenicity of Multi-Target Chimeric RHDV Virus-Like Particles Delivering Foreign B-Cell Epitopes |
| title_sort | immunogenicity of multi target chimeric rhdv virus like particles delivering foreign b cell epitopes |
| topic | virus-like particles (VLPs) chimeric VLPs nanoparticles vaccine platform multivalent vaccine RHDV |
| url | https://www.mdpi.com/2076-393X/10/2/229 |
| work_keys_str_mv | AT mariazamoraceballos immunogenicityofmultitargetchimericrhdvviruslikeparticlesdeliveringforeignbcellepitopes AT noeliamoreno immunogenicityofmultitargetchimericrhdvviruslikeparticlesdeliveringforeignbcellepitopes AT davidgilcantero immunogenicityofmultitargetchimericrhdvviruslikeparticlesdeliveringforeignbcellepitopes AT josercaston immunogenicityofmultitargetchimericrhdvviruslikeparticlesdeliveringforeignbcellepitopes AT estherblanco immunogenicityofmultitargetchimericrhdvviruslikeparticlesdeliveringforeignbcellepitopes AT juanbarcena immunogenicityofmultitargetchimericrhdvviruslikeparticlesdeliveringforeignbcellepitopes |