Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective Study
Yidan Sun,1– 3,* Xiaozhou Yao,1– 3,* Yingmeng Ni,1– 3 Yibing Peng,4 Guochao Shi1– 3 1Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Institute of Respiratory Diseases, Shangh...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-12-01
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| Series: | Infection and Drug Resistance |
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| Online Access: | https://www.dovepress.com/diagnostic-efficacy-of-t-spottb-for-active-tuberculosis-in-adult-a-ret-peer-reviewed-fulltext-article-IDR |
| _version_ | 1811190133202878464 |
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| author | Sun Y Yao X Ni Y Peng Y Shi G |
| author_facet | Sun Y Yao X Ni Y Peng Y Shi G |
| author_sort | Sun Y |
| collection | DOAJ |
| description | Yidan Sun,1– 3,* Xiaozhou Yao,1– 3,* Yingmeng Ni,1– 3 Yibing Peng,4 Guochao Shi1– 3 1Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Institute of Respiratory Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases, Shanghai, People’s Republic of China; 4Faculty of Medical Laboratory Science, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guochao Shi, Department of Respiratory and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Er Road, Shanghai, 200025, People’s Republic of China, Tel +86-13918462035, Fax + 021-64314162, Email shiguochao@hotmail.com Yibing Peng, Faculty of Medical Laboratory Science, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Er Road, Shanghai, 200025, People’s Republic of China, Tel +86-13795243560, Email pyb9861@sina.comPurpose: To explore the diagnostic efficacy and optimal diagnosis threshold of T-SPOT.TB for active tuberculosis in adults and to evaluate the influential factors for T-SPOT.TB results.Patients and Methods: A retrospective study of 1193 adult inpatients from April 2015 to March 2018 in Ruijin Hospital was conducted. All included patients underwent T-SPOT.TB assay, and were divided into two groups, active tuberculosis (ATB) and non-active tuberculosis (non-ATB) groups. Their demographic data, underlying diseases, personal history and laboratory findings were collected to calculate the diagnostic efficacy at different diagnosis thresholds and analyze the impact factors. Symptoms and imaging features of ATB patients were recorded and analyzed.Results: A total of 114 ATB patients and 1079 non-ATB patients were included in the study, and ATB patients had a higher level of T-SPOT.TB than the non-ATB group. Sensitivity and specificity of T-SPOT.TB for diagnosing ATB are 78.95% and 68.58% as the threshold at 6sfu. In the diagnosis accordance curves, ESAT-6, CFP-10, and max (ESAT-6 or CFP-10) reached the plateau at 40sfu, while sum (ESAT-6 and CFP-10) reached the plateau at 70sfu. Multivariate logistic regression analysis showed that obsolescent tuberculosis (p=0.001), smoking history(p=0.005), diabetes(p=0.035) and advanced age (≥ 65 years old) (p=0.031) were risk factors for false-positive result of T-SPOT.TB. In terms of imaging features, logistic regression analysis suggested that the thin-wall cavitary lesion was the only feature associated with the result of T-SPOT.TB.Conclusion: As for using T-SPOT.TB test to diagnose active tuberculosis, increased threshold could significantly elevate the diagnosis accordance. And we suggest that the threshold of T-SPOT.TB could be increased to 40sfu for diagnosing ATB. Attention should be paid when diagnose ATB in population with obsolescent tuberculosis, smoking history, diabetes and advanced age, for the risk of false-positive.Keywords: T-SPOT.TB, active tuberculosis, diagnosis threshold |
| first_indexed | 2024-04-11T14:46:10Z |
| format | Article |
| id | doaj.art-a66f5ad66d824702a432b2b32d6a32db |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-11T14:46:10Z |
| publishDate | 2022-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-a66f5ad66d824702a432b2b32d6a32db2022-12-22T04:17:38ZengDove Medical PressInfection and Drug Resistance1178-69732022-12-01Volume 157077709380053Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective StudySun YYao XNi YPeng YShi GYidan Sun,1– 3,* Xiaozhou Yao,1– 3,* Yingmeng Ni,1– 3 Yibing Peng,4 Guochao Shi1– 3 1Department of Pulmonary and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 2Institute of Respiratory Diseases, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China; 3Shanghai Key Laboratory of Emergency Prevention, Diagnosis and Treatment of Respiratory Infectious Diseases, Shanghai, People’s Republic of China; 4Faculty of Medical Laboratory Science, Shanghai Jiao Tong University School of Medicine, Shanghai, People’s Republic of China*These authors contributed equally to this workCorrespondence: Guochao Shi, Department of Respiratory and Critical Care Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Er Road, Shanghai, 200025, People’s Republic of China, Tel +86-13918462035, Fax + 021-64314162, Email shiguochao@hotmail.com Yibing Peng, Faculty of Medical Laboratory Science, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, No. 197 Ruijin Er Road, Shanghai, 200025, People’s Republic of China, Tel +86-13795243560, Email pyb9861@sina.comPurpose: To explore the diagnostic efficacy and optimal diagnosis threshold of T-SPOT.TB for active tuberculosis in adults and to evaluate the influential factors for T-SPOT.TB results.Patients and Methods: A retrospective study of 1193 adult inpatients from April 2015 to March 2018 in Ruijin Hospital was conducted. All included patients underwent T-SPOT.TB assay, and were divided into two groups, active tuberculosis (ATB) and non-active tuberculosis (non-ATB) groups. Their demographic data, underlying diseases, personal history and laboratory findings were collected to calculate the diagnostic efficacy at different diagnosis thresholds and analyze the impact factors. Symptoms and imaging features of ATB patients were recorded and analyzed.Results: A total of 114 ATB patients and 1079 non-ATB patients were included in the study, and ATB patients had a higher level of T-SPOT.TB than the non-ATB group. Sensitivity and specificity of T-SPOT.TB for diagnosing ATB are 78.95% and 68.58% as the threshold at 6sfu. In the diagnosis accordance curves, ESAT-6, CFP-10, and max (ESAT-6 or CFP-10) reached the plateau at 40sfu, while sum (ESAT-6 and CFP-10) reached the plateau at 70sfu. Multivariate logistic regression analysis showed that obsolescent tuberculosis (p=0.001), smoking history(p=0.005), diabetes(p=0.035) and advanced age (≥ 65 years old) (p=0.031) were risk factors for false-positive result of T-SPOT.TB. In terms of imaging features, logistic regression analysis suggested that the thin-wall cavitary lesion was the only feature associated with the result of T-SPOT.TB.Conclusion: As for using T-SPOT.TB test to diagnose active tuberculosis, increased threshold could significantly elevate the diagnosis accordance. And we suggest that the threshold of T-SPOT.TB could be increased to 40sfu for diagnosing ATB. Attention should be paid when diagnose ATB in population with obsolescent tuberculosis, smoking history, diabetes and advanced age, for the risk of false-positive.Keywords: T-SPOT.TB, active tuberculosis, diagnosis thresholdhttps://www.dovepress.com/diagnostic-efficacy-of-t-spottb-for-active-tuberculosis-in-adult-a-ret-peer-reviewed-fulltext-article-IDRt-spot.tbactive tuberculosisdiagnosis threshold |
| spellingShingle | Sun Y Yao X Ni Y Peng Y Shi G Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective Study Infection and Drug Resistance t-spot.tb active tuberculosis diagnosis threshold |
| title | Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective Study |
| title_full | Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective Study |
| title_fullStr | Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective Study |
| title_full_unstemmed | Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective Study |
| title_short | Diagnostic Efficacy of T-SPOT.TB for Active Tuberculosis in Adult: A Retrospective Study |
| title_sort | diagnostic efficacy of t spot tb for active tuberculosis in adult a retrospective study |
| topic | t-spot.tb active tuberculosis diagnosis threshold |
| url | https://www.dovepress.com/diagnostic-efficacy-of-t-spottb-for-active-tuberculosis-in-adult-a-ret-peer-reviewed-fulltext-article-IDR |
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