Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay
African swine fever (ASF) is a contagious disease of wild boar and domestic pigs notifiable to the World Organisation for Animal Health due to its high socio-economic impact. ASF is caused by the complex ASF virus (ASFV), and it can present different clinical manifestations that can be confused with...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-03-01
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| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/12/3/307 |
| _version_ | 1797239116998377472 |
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| author | Cristina Aira Gabriela González-García Juan Martínez-Cano Nuria de la Roja Monica Giammarioli Francesco Feliziani Žanete Šteingolde Jurate Buitkuviene Petr Václavek Dimitrije Glišić Carmina Gallardo Patricia Sastre Marga García-Durán Paloma Rueda Alba Fresco-Taboada |
| author_facet | Cristina Aira Gabriela González-García Juan Martínez-Cano Nuria de la Roja Monica Giammarioli Francesco Feliziani Žanete Šteingolde Jurate Buitkuviene Petr Václavek Dimitrije Glišić Carmina Gallardo Patricia Sastre Marga García-Durán Paloma Rueda Alba Fresco-Taboada |
| author_sort | Cristina Aira |
| collection | DOAJ |
| description | African swine fever (ASF) is a contagious disease of wild boar and domestic pigs notifiable to the World Organisation for Animal Health due to its high socio-economic impact. ASF is caused by the complex ASF virus (ASFV), and it can present different clinical manifestations that can be confused with other diseases; for this reason, laboratory testing is necessary for the proper diagnosis of clinically suspected animals. Despite the efforts put into it over decades, no treatment or safe vaccine is globally available, and disease control is based on early diagnosis and the implementation of strict biosecurity measures. In this context, rapid tests have the potential to accelerate and facilitate the identification of infected animals by giving fast on-site results. In this work, we improved the available point-of-care assays for the diagnosis of the disease by the development of a more specific antigen test and a more sensitive antibody test. This antibody detection test allowed for the earlier detection of infected animals than two commercial indirect ELISAs (statistically significant). Moreover, we developed a combined dual rapid test, unifying, in the same cassette, an antigen detection strip and an antibody detection strip. In this study, we confirmed that this combo approach is a useful tool for implementing rapid tests in the field since it increases the percentage of positive samples detected, even when PCR turns negative, while maintaining a good specificity. |
| first_indexed | 2024-04-24T17:46:26Z |
| format | Article |
| id | doaj.art-a66f5d7a52aa4294aabf19a9679cc106 |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-04-24T17:46:26Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-a66f5d7a52aa4294aabf19a9679cc1062024-03-27T14:07:06ZengMDPI AGVaccines2076-393X2024-03-0112330710.3390/vaccines12030307Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow AssayCristina Aira0Gabriela González-García1Juan Martínez-Cano2Nuria de la Roja3Monica Giammarioli4Francesco Feliziani5Žanete Šteingolde6Jurate Buitkuviene7Petr Václavek8Dimitrije Glišić9Carmina Gallardo10Patricia Sastre11Marga García-Durán12Paloma Rueda13Alba Fresco-Taboada14Gold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainGold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainGold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainGold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati” (IZSUM), Via Salvemini 1, 06126 Perugia, ItalyIstituto Zooprofilattico Sperimentale dell’Umbria e delle Marche “Togo Rosati” (IZSUM), Via Salvemini 1, 06126 Perugia, ItalyInstitute of Food Safety, Animal Health and Environment “BIOR”, Lejupes Street 3, LV-1076 Riga, LatviaNational Food and Veterinary Risk Assessment Institute, J. Kairiūkščio g. 10, LT-08409 Vilnius, LithuaniaDepartment of Virology, State Veterinary Institute Jihlava, Rantirovska 93/20, Horni Kosov, 586 01 Jihlava, Czech RepublicVirology Department, Institute of Veterinary Medicine of Serbia, Janisa Janulisa 14, 11000 Belgrade, SerbiaEuropean Union Reference Laboratory for African Swine Fever, Centro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Carretera Algete-El Casar de Talamanca, Km. 8.1, 28130 Madrid, SpainGold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainGold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainGold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainGold Standard Diagnostics Madrid (GSD Madrid), Calle de los Hermanos García Noblejas 39, 28037 Madrid, SpainAfrican swine fever (ASF) is a contagious disease of wild boar and domestic pigs notifiable to the World Organisation for Animal Health due to its high socio-economic impact. ASF is caused by the complex ASF virus (ASFV), and it can present different clinical manifestations that can be confused with other diseases; for this reason, laboratory testing is necessary for the proper diagnosis of clinically suspected animals. Despite the efforts put into it over decades, no treatment or safe vaccine is globally available, and disease control is based on early diagnosis and the implementation of strict biosecurity measures. In this context, rapid tests have the potential to accelerate and facilitate the identification of infected animals by giving fast on-site results. In this work, we improved the available point-of-care assays for the diagnosis of the disease by the development of a more specific antigen test and a more sensitive antibody test. This antibody detection test allowed for the earlier detection of infected animals than two commercial indirect ELISAs (statistically significant). Moreover, we developed a combined dual rapid test, unifying, in the same cassette, an antigen detection strip and an antibody detection strip. In this study, we confirmed that this combo approach is a useful tool for implementing rapid tests in the field since it increases the percentage of positive samples detected, even when PCR turns negative, while maintaining a good specificity.https://www.mdpi.com/2076-393X/12/3/307African swine feverantigen detectionantibody detectionrapid testpoint of care |
| spellingShingle | Cristina Aira Gabriela González-García Juan Martínez-Cano Nuria de la Roja Monica Giammarioli Francesco Feliziani Žanete Šteingolde Jurate Buitkuviene Petr Václavek Dimitrije Glišić Carmina Gallardo Patricia Sastre Marga García-Durán Paloma Rueda Alba Fresco-Taboada Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay Vaccines African swine fever antigen detection antibody detection rapid test point of care |
| title | Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay |
| title_full | Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay |
| title_fullStr | Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay |
| title_full_unstemmed | Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay |
| title_short | Simultaneous Detection of Antigen and Antibodies of African Swine Fever in a Novel Combo Lateral Flow Assay |
| title_sort | simultaneous detection of antigen and antibodies of african swine fever in a novel combo lateral flow assay |
| topic | African swine fever antigen detection antibody detection rapid test point of care |
| url | https://www.mdpi.com/2076-393X/12/3/307 |
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