Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα Ectodomain
To estimate the levels of serum IgE that is actually able to be bound to FcεRI, we developed a novel enzyme-linked immunosorbent assay (ELISA) using a recombinant soluble form of the human FcεRIα ectodomain (soluble α-ELISA). We evaluated the levels of FcεRI-bindable serum IgE in normal volunteers a...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
1997-01-01
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| Series: | Allergology International |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1323893015315963 |
| _version_ | 1819267495906770944 |
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| author | Naruhito Wada Ko Okumura Chisei Ra |
| author_facet | Naruhito Wada Ko Okumura Chisei Ra |
| author_sort | Naruhito Wada |
| collection | DOAJ |
| description | To estimate the levels of serum IgE that is actually able to be bound to FcεRI, we developed a novel enzyme-linked immunosorbent assay (ELISA) using a recombinant soluble form of the human FcεRIα ectodomain (soluble α-ELISA). We evaluated the levels of FcεRI-bindable serum IgE in normal volunteers and in patients with atopic dermatitis and bronchial asthma, and compared them with those of total IgE measured by a conventional sandwich-ELISA. There was a striking difference between the IgE values evaluated with these two ELISA systems. In most of the sera, the IgE values evaluated with the soluble α-ELISA were substantially lower than those evaluated with the sandwich-ELISA. Our results indicate that all serum IgE (especially in hyper-lgE sera) does not necessarily bind to the FcεRI and that it will be useful to evaluate FcεRI-bindable IgE for further analysis of allergic states. |
| first_indexed | 2024-12-23T21:18:05Z |
| format | Article |
| id | doaj.art-a6701a75ba0a4838a867ad4a9a551005 |
| institution | Directory Open Access Journal |
| issn | 1323-8930 |
| language | English |
| last_indexed | 2024-12-23T21:18:05Z |
| publishDate | 1997-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Allergology International |
| spelling | doaj.art-a6701a75ba0a4838a867ad4a9a5510052022-12-21T17:30:50ZengElsevierAllergology International1323-89301997-01-0146317318010.2332/allergolint.46.173Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα EctodomainNaruhito WadaKo OkumuraChisei RaTo estimate the levels of serum IgE that is actually able to be bound to FcεRI, we developed a novel enzyme-linked immunosorbent assay (ELISA) using a recombinant soluble form of the human FcεRIα ectodomain (soluble α-ELISA). We evaluated the levels of FcεRI-bindable serum IgE in normal volunteers and in patients with atopic dermatitis and bronchial asthma, and compared them with those of total IgE measured by a conventional sandwich-ELISA. There was a striking difference between the IgE values evaluated with these two ELISA systems. In most of the sera, the IgE values evaluated with the soluble α-ELISA were substantially lower than those evaluated with the sandwich-ELISA. Our results indicate that all serum IgE (especially in hyper-lgE sera) does not necessarily bind to the FcεRI and that it will be useful to evaluate FcεRI-bindable IgE for further analysis of allergic states.http://www.sciencedirect.com/science/article/pii/S1323893015315963allergyenzyme-linked immunosorbent assayFcεRIFcεRI-bindable IgEimmunoglobulin Esoluble human FcεRIα ectodomain |
| spellingShingle | Naruhito Wada Ko Okumura Chisei Ra Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα Ectodomain Allergology International allergy enzyme-linked immunosorbent assay FcεRI FcεRI-bindable IgE immunoglobulin E soluble human FcεRIα ectodomain |
| title | Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα Ectodomain |
| title_full | Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα Ectodomain |
| title_fullStr | Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα Ectodomain |
| title_full_unstemmed | Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα Ectodomain |
| title_short | Evaluation of FcεRI-Bindable Human Ige With an Enzyme-Linked Immunosorbent Assay Using a Recombinant Soluble form of the Human FcεRIα Ectodomain |
| title_sort | evaluation of fcεri bindable human ige with an enzyme linked immunosorbent assay using a recombinant soluble form of the human fcεriα ectodomain |
| topic | allergy enzyme-linked immunosorbent assay FcεRI FcεRI-bindable IgE immunoglobulin E soluble human FcεRIα ectodomain |
| url | http://www.sciencedirect.com/science/article/pii/S1323893015315963 |
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