Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters
Aim of study: To investigate the general response patterns of the borage plant to water fluctuations from a biochemical and physiological perspective. Area of study: East Azerbaijan Province of Iran during the period 2012 and 2013. Material and methods: The study investigated the effects of i...
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| Format: | Article |
| Language: | English |
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Consejo Superior de Investigaciones Científicas (CSIC)
2022-06-01
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| Series: | Spanish Journal of Agricultural Research |
| Subjects: | |
| Online Access: | https://revistas.inia.es/index.php/sjar/article/view/19132 |
| _version_ | 1827203609061752832 |
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| author | Soheila Dastborhan Kazem Ghassemi-Golezani Andrzej Kalisz Mostafa Valizadeh Behnam Asgari Lajayer Tessema Astatkie |
| author_facet | Soheila Dastborhan Kazem Ghassemi-Golezani Andrzej Kalisz Mostafa Valizadeh Behnam Asgari Lajayer Tessema Astatkie |
| author_sort | Soheila Dastborhan |
| collection | DOAJ |
| description |
Aim of study: To investigate the general response patterns of the borage plant to water fluctuations from a biochemical and physiological perspective.
Area of study: East Azerbaijan Province of Iran during the period 2012 and 2013.
Material and methods: The study investigated the effects of irrigation (after 60, 90, 120 and 150 mm evaporation) and priming (unprimed, and primed seeds with water, 1% KNO3 and 1% KH2PO4) on the antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters of borage using a split-plot experimental design.
Main results: The statistical analyses showed no effect of seed priming on all evaluated traits other than than extracellular superoxide dismutase SOD3 activity where it was significantly enhanced by seed pretreatment with 1% KNO3 and 1% KH2PO4. However, irrigations after 120 and 150 mm evaporation increased Cu/Zn-superoxide dismutase (SOD1), SOD2, and SOD3, soluble sugars, and initial fluorescence (F0). The mean contents of Ch a, Ch b, and Ch a+Ch b under mild, moderate and severe water deficit were significantly higher than those under normal irrigation. Severe drought stress gave the highest carotenoids content and quantum yield baseline parameter (F0/Fm) of borage leaves. However, water limitation decreased Chl a/Chl b ratio, maximum primary yield of photosystem II (Fv/F0), and maximum quantum yield of photosystem II (Fv/Fm).
Research highlights: Based on these findings, it is postulated that the increase in soluble sugars and SOD activity under stress, and the accumulation of carotenoids under severe water limitation indirectly enhance the tolerance of borage plants to drought stress.
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| first_indexed | 2024-04-11T21:03:11Z |
| format | Article |
| id | doaj.art-a672e1ae38744d7298f01c4d74ba9625 |
| institution | Directory Open Access Journal |
| issn | 2171-9292 |
| language | English |
| last_indexed | 2025-03-21T11:39:12Z |
| publishDate | 2022-06-01 |
| publisher | Consejo Superior de Investigaciones Científicas (CSIC) |
| record_format | Article |
| series | Spanish Journal of Agricultural Research |
| spelling | doaj.art-a672e1ae38744d7298f01c4d74ba96252024-07-02T03:15:56ZengConsejo Superior de Investigaciones Científicas (CSIC)Spanish Journal of Agricultural Research2171-92922022-06-0120310.5424/sjar/2022203-19132Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parametersSoheila Dastborhan0Kazem Ghassemi-Golezani1Andrzej Kalisz2Mostafa Valizadeh3Behnam Asgari Lajayer4Tessema Astatkie5University of Tabriz, Faculty of Agriculture, Dept. of Plant Ecophysiology, Tabriz, IranUniversity of Tabriz, Faculty of Agriculture, Dept. of Plant Ecophysiology, Tabriz, IranUniversity of Agriculture in Krakow, Faculty of Biotechnology and Horticulture, Dept. of Horticulture, Kraków, Poland. University of Tabriz, Faculty of Agriculture, Dept. of Plant Breeding and Biotechnology, Tabriz, IranUniversity of Tabriz, Faculty of Agriculture, Dept. of Soil Science, Tabriz, IranDalhousie University, Faculty of Agriculture, Truro, NS, B2N 5E3, Canada Aim of study: To investigate the general response patterns of the borage plant to water fluctuations from a biochemical and physiological perspective. Area of study: East Azerbaijan Province of Iran during the period 2012 and 2013. Material and methods: The study investigated the effects of irrigation (after 60, 90, 120 and 150 mm evaporation) and priming (unprimed, and primed seeds with water, 1% KNO3 and 1% KH2PO4) on the antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters of borage using a split-plot experimental design. Main results: The statistical analyses showed no effect of seed priming on all evaluated traits other than than extracellular superoxide dismutase SOD3 activity where it was significantly enhanced by seed pretreatment with 1% KNO3 and 1% KH2PO4. However, irrigations after 120 and 150 mm evaporation increased Cu/Zn-superoxide dismutase (SOD1), SOD2, and SOD3, soluble sugars, and initial fluorescence (F0). The mean contents of Ch a, Ch b, and Ch a+Ch b under mild, moderate and severe water deficit were significantly higher than those under normal irrigation. Severe drought stress gave the highest carotenoids content and quantum yield baseline parameter (F0/Fm) of borage leaves. However, water limitation decreased Chl a/Chl b ratio, maximum primary yield of photosystem II (Fv/F0), and maximum quantum yield of photosystem II (Fv/Fm). Research highlights: Based on these findings, it is postulated that the increase in soluble sugars and SOD activity under stress, and the accumulation of carotenoids under severe water limitation indirectly enhance the tolerance of borage plants to drought stress. https://revistas.inia.es/index.php/sjar/article/view/19132Borago officinalis L.chlorophyllcompatible solutesdrought stressfluorescencesuperoxide dismutase |
| spellingShingle | Soheila Dastborhan Kazem Ghassemi-Golezani Andrzej Kalisz Mostafa Valizadeh Behnam Asgari Lajayer Tessema Astatkie Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters Spanish Journal of Agricultural Research Borago officinalis L. chlorophyll compatible solutes drought stress fluorescence superoxide dismutase |
| title | Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters |
| title_full | Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters |
| title_fullStr | Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters |
| title_full_unstemmed | Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters |
| title_short | Biochemical and physiological response of borage to seed priming and water deficit: antioxidant enzymes, osmolytes, photosynthetic pigments, and fluorescence parameters |
| title_sort | biochemical and physiological response of borage to seed priming and water deficit antioxidant enzymes osmolytes photosynthetic pigments and fluorescence parameters |
| topic | Borago officinalis L. chlorophyll compatible solutes drought stress fluorescence superoxide dismutase |
| url | https://revistas.inia.es/index.php/sjar/article/view/19132 |
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