Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine
ABSTRACTPertussis caused by Bordetella pertussis, remains a public health problem worldwide, despite high vaccine coverage in infants and children in many countries. Iran has been using whole cell vaccine for the last 50 years with more than 95% vaccination rate since 1988 and has experienced pertus...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1665479 |
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| author | Azadeh Safarchi Sophie Octavia Vajihe Sadat Nikbin Masoumeh Nakhost Lotfi Seyed Mohsen Zahraei Chin Yen Tay Binit Lamichhane Fereshteh Shahcheraghi Ruiting Lan |
| author_facet | Azadeh Safarchi Sophie Octavia Vajihe Sadat Nikbin Masoumeh Nakhost Lotfi Seyed Mohsen Zahraei Chin Yen Tay Binit Lamichhane Fereshteh Shahcheraghi Ruiting Lan |
| author_sort | Azadeh Safarchi |
| collection | DOAJ |
| description | ABSTRACTPertussis caused by Bordetella pertussis, remains a public health problem worldwide, despite high vaccine coverage in infants and children in many countries. Iran has been using whole cell vaccine for the last 50 years with more than 95% vaccination rate since 1988 and has experienced pertussis resurgence in recent years. Here, we sequenced 55 B. pertussis isolates mostly collected from three provinces with the highest number of pertussis cases in Iran, including Tehran, Mazandaran, and Eastern-Azarbayjan from the period of 2008-2016. Most isolates carried ptxP3/prn2 alleles (42/55, 76%), the same genotype as isolates circulating in acellular vaccine-administrating countries. The second most frequent genotype was ptxP3/prn9 (8/55, 14%). Only three isolates (5%) were ptxP1. Phylogenetic analysis showed that Iranian ptxP3 isolates can be divided into eight clades (Clades 1-8) with no temporal association. Most of the isolates from Tehran grouped together as one distinctive clade (Clade 8) with six unique single nucleotide polymorphisms (SNPs). In addition, the prn9 isolates were grouped together as Clade 5 with 12 clade-supporting SNPs. No pertactin deficient isolates were found among the 55 Iranian isolates. Our findings suggest that there is an ongoing adaptation and evolution of B. pertussis regardless of the types of vaccine used. |
| first_indexed | 2024-03-08T21:59:14Z |
| format | Article |
| id | doaj.art-a67563cfbcf648f8baf90f98bef4ee7f |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:59:14Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-a67563cfbcf648f8baf90f98bef4ee7f2023-12-19T16:09:57ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-01811416142710.1080/22221751.2019.1665479Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccineAzadeh Safarchi0Sophie Octavia1Vajihe Sadat Nikbin2Masoumeh Nakhost Lotfi3Seyed Mohsen Zahraei4Chin Yen Tay5Binit Lamichhane6Fereshteh Shahcheraghi7Ruiting Lan8Pertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran, Islamic Republic of IranSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, AustraliaPertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran, Islamic Republic of IranPertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran, Islamic Republic of IranCentre for Communicable Disease Control, Ministry of Health and Medical Education, Tehran, Islamic Republic of IranPathology and Laboratory Medicine, University of Western Australia, Perth, AustraliaPathology and Laboratory Medicine, University of Western Australia, Perth, AustraliaPertussis Reference Laboratory, Department of Bacteriology, Pasteur Institute of Iran, Tehran, Islamic Republic of IranSchool of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, AustraliaABSTRACTPertussis caused by Bordetella pertussis, remains a public health problem worldwide, despite high vaccine coverage in infants and children in many countries. Iran has been using whole cell vaccine for the last 50 years with more than 95% vaccination rate since 1988 and has experienced pertussis resurgence in recent years. Here, we sequenced 55 B. pertussis isolates mostly collected from three provinces with the highest number of pertussis cases in Iran, including Tehran, Mazandaran, and Eastern-Azarbayjan from the period of 2008-2016. Most isolates carried ptxP3/prn2 alleles (42/55, 76%), the same genotype as isolates circulating in acellular vaccine-administrating countries. The second most frequent genotype was ptxP3/prn9 (8/55, 14%). Only three isolates (5%) were ptxP1. Phylogenetic analysis showed that Iranian ptxP3 isolates can be divided into eight clades (Clades 1-8) with no temporal association. Most of the isolates from Tehran grouped together as one distinctive clade (Clade 8) with six unique single nucleotide polymorphisms (SNPs). In addition, the prn9 isolates were grouped together as Clade 5 with 12 clade-supporting SNPs. No pertactin deficient isolates were found among the 55 Iranian isolates. Our findings suggest that there is an ongoing adaptation and evolution of B. pertussis regardless of the types of vaccine used.https://www.tandfonline.com/doi/10.1080/22221751.2019.1665479Bordetella pertussisIranwhole genome sequencinggenomicsadaptation and evolution |
| spellingShingle | Azadeh Safarchi Sophie Octavia Vajihe Sadat Nikbin Masoumeh Nakhost Lotfi Seyed Mohsen Zahraei Chin Yen Tay Binit Lamichhane Fereshteh Shahcheraghi Ruiting Lan Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine Emerging Microbes and Infections Bordetella pertussis Iran whole genome sequencing genomics adaptation and evolution |
| title | Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine |
| title_full | Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine |
| title_fullStr | Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine |
| title_full_unstemmed | Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine |
| title_short | Genomic epidemiology of Iranian Bordetella pertussis: 50 years after the implementation of whole cell vaccine |
| title_sort | genomic epidemiology of iranian bordetella pertussis 50 years after the implementation of whole cell vaccine |
| topic | Bordetella pertussis Iran whole genome sequencing genomics adaptation and evolution |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1665479 |
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