Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production
Aromas and flavours can be produced from fungi by either de novo synthesis or biotransformation processes. Herein, the biocatalytic potential of seven basidiomycete species from Colombia fungal strains isolated as endophytes or basidioma was evaluated. <i>Ganoderma webenarium</i>, <i&...
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| Format: | Article |
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MDPI AG
2020-09-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/18/4344 |
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| author | David A. Jaramillo María J. Méndez Gabriela Vargas Elena E. Stashenko Aída-M. Vasco-Palacios Andrés Ceballos Nelson H. Caicedo |
| author_facet | David A. Jaramillo María J. Méndez Gabriela Vargas Elena E. Stashenko Aída-M. Vasco-Palacios Andrés Ceballos Nelson H. Caicedo |
| author_sort | David A. Jaramillo |
| collection | DOAJ |
| description | Aromas and flavours can be produced from fungi by either de novo synthesis or biotransformation processes. Herein, the biocatalytic potential of seven basidiomycete species from Colombia fungal strains isolated as endophytes or basidioma was evaluated. <i>Ganoderma webenarium</i>, <i>Ganoderma chocoense</i>, and <i>Ganoderma stipitatum</i> were the most potent strains capable of decolourizing β,β-carotene as evidence of their potential as biocatalysts for de novo aroma synthesis. Since a species’ biocatalytic potential cannot solely be determined via qualitative screening using β,β-carotene biotransformation processes, we focused on using α-pinene biotransformation with mycelium as a measure of catalytic potential. Here, two strains of <i>Trametes elegans</i>—namely, the endophytic (ET-06) and basidioma (EBB-046) strains—were screened. Herein, <i>T. elegans</i> is reported for the first time as a novel biocatalyst for the oxidation of α-pinene, with a product yield of 2.9 mg of <i>cis</i>-Verbenol per gram of dry weight mycelia used. The EBB-046 strain generated flavour compounds via the biotransformation of a Cape gooseberry medium and de novo synthesis in submerged cultures. Three aroma-producing compounds were identified via GC–MS—namely, methyl-3-methoxy-4H-pyran-4-one, hexahydro-3-(methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione, and hexahydro-3-(methylphenyl)-pyrrolo[1,2-a]pyrazine-1,4-dione. |
| first_indexed | 2024-03-10T16:08:55Z |
| format | Article |
| id | doaj.art-a677e07a2eaf48328c035656c88707ba |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T16:08:55Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a677e07a2eaf48328c035656c88707ba2023-11-20T14:39:53ZengMDPI AGMolecules1420-30492020-09-012518434410.3390/molecules25184344Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma ProductionDavid A. Jaramillo0María J. Méndez1Gabriela Vargas2Elena E. Stashenko3Aída-M. Vasco-Palacios4Andrés Ceballos5Nelson H. Caicedo6Department of Biochemical Engineering, Universidad Icesi, Calle 18 No. 122–135 Pance, Cali 760031, ColombiaDepartment of Biochemical Engineering, Universidad Icesi, Calle 18 No. 122–135 Pance, Cali 760031, ColombiaDepartment of Biochemical Engineering, Universidad Icesi, Calle 18 No. 122–135 Pance, Cali 760031, ColombiaUniversidad Industrial de Santander. Chromatography and Mass Spectrometry Center, Calle 9 Carrera 27, Bucaramanga 680002, ColombiaGrupo de Microbiología Ambiental—BioMicro, Escuela de Microbiología, Universidad de Antioquia, UdeA, Calle 70 No. 52–21, Medellín 050010, ColombiaDepartment of Biochemical Engineering, Universidad Icesi, Calle 18 No. 122–135 Pance, Cali 760031, ColombiaDepartment of Biochemical Engineering, Universidad Icesi, Calle 18 No. 122–135 Pance, Cali 760031, ColombiaAromas and flavours can be produced from fungi by either de novo synthesis or biotransformation processes. Herein, the biocatalytic potential of seven basidiomycete species from Colombia fungal strains isolated as endophytes or basidioma was evaluated. <i>Ganoderma webenarium</i>, <i>Ganoderma chocoense</i>, and <i>Ganoderma stipitatum</i> were the most potent strains capable of decolourizing β,β-carotene as evidence of their potential as biocatalysts for de novo aroma synthesis. Since a species’ biocatalytic potential cannot solely be determined via qualitative screening using β,β-carotene biotransformation processes, we focused on using α-pinene biotransformation with mycelium as a measure of catalytic potential. Here, two strains of <i>Trametes elegans</i>—namely, the endophytic (ET-06) and basidioma (EBB-046) strains—were screened. Herein, <i>T. elegans</i> is reported for the first time as a novel biocatalyst for the oxidation of α-pinene, with a product yield of 2.9 mg of <i>cis</i>-Verbenol per gram of dry weight mycelia used. The EBB-046 strain generated flavour compounds via the biotransformation of a Cape gooseberry medium and de novo synthesis in submerged cultures. Three aroma-producing compounds were identified via GC–MS—namely, methyl-3-methoxy-4H-pyran-4-one, hexahydro-3-(methylpropyl)-pyrrolo[1,2-a]pyrazine-1,4-dione, and hexahydro-3-(methylphenyl)-pyrrolo[1,2-a]pyrazine-1,4-dione.https://www.mdpi.com/1420-3049/25/18/4344aromasbasidiomycetesbioprospectionα-Pinene<i>cis</i>-verbenol |
| spellingShingle | David A. Jaramillo María J. Méndez Gabriela Vargas Elena E. Stashenko Aída-M. Vasco-Palacios Andrés Ceballos Nelson H. Caicedo Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production Molecules aromas basidiomycetes bioprospection α-Pinene <i>cis</i>-verbenol |
| title | Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production |
| title_full | Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production |
| title_fullStr | Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production |
| title_full_unstemmed | Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production |
| title_short | Biocatalytic Potential of Native Basidiomycetes from Colombia for Flavour/Aroma Production |
| title_sort | biocatalytic potential of native basidiomycetes from colombia for flavour aroma production |
| topic | aromas basidiomycetes bioprospection α-Pinene <i>cis</i>-verbenol |
| url | https://www.mdpi.com/1420-3049/25/18/4344 |
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