Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea)
<i>Ampelopsis grossedentata</i> (vine tea) has been used as a detoxifying beverage in China for centuries. To systematically identify its bioactive compounds, the study adopted standards-based ultra-high-performance liquid chromatography coupled with quadrupole/electrostatic field orbitr...
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2022-10-01
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| author | Rongxin Cai Xican Li Chunhou Li Jiayi Zhu Jingyuan Zeng Jianwu Li Boxu Tang Zheng Li Shuqin Liu Yan Yan |
| author_facet | Rongxin Cai Xican Li Chunhou Li Jiayi Zhu Jingyuan Zeng Jianwu Li Boxu Tang Zheng Li Shuqin Liu Yan Yan |
| author_sort | Rongxin Cai |
| collection | DOAJ |
| description | <i>Ampelopsis grossedentata</i> (vine tea) has been used as a detoxifying beverage in China for centuries. To systematically identify its bioactive compounds, the study adopted standards-based ultra-high-performance liquid chromatography coupled with quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive Orbitrap MS) analysis. The analysis was conducted under a negative ion model and the data were collected using the Xcalibur 4.1 software package. Based on comparisons with authentic standards, 36 bioactive compounds were putatively identified by four parameters: retention time, molecular ion peak, MS/MS profile, and characteristic fragments. These bioactive compounds include two chromones (noreugenin and 3,5,7-trihydroxychromone), 15 flavonoids (S-eriodictyol, S-naringenin, luteolin, ampelopsin, taxifolin, myricetin, quercetin, viscidulin I, kaempferol, myricetin 3-<i>O</i>-galactoside, myricitrin, avicularin, quercitrin, isorhamnetin-3-<i>O</i>-<i>β</i>-D-glucoside, and afzelin), four phenolic acids (gallic acid, 3,4-dihydroxy-5-methoxybenzoic acid, syringic acid, and ellagic acid), five tea polyphenols (epigallocatechin, epigallocatechin gallate, gallocatechin gallate, epicatechin gallate, and catechin gallate), three chalcones (phloridzin, phloretin, and naringenin chalcone), one stilbene (polydatin), two lipids (myristic acid and ethyl stearate), one sugar (D-gluconic acid), one amino acid (L-tryptophan), one triterpenoid (oleanolic acid) and one alkaloid (jervine). Notably, the jervine identification is the first report regarding the occurrence of alkaloid in the plant. Two chromones may be the parent skeleton to biosynthesize the flavonoid in <i>A. grossedentata.</i> |
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| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-09T18:39:21Z |
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| spelling | doaj.art-a67bd8c22c1a434a9daa9415c15240a42023-11-24T06:50:27ZengMDPI AGSeparations2297-87392022-10-0191132910.3390/separations9110329Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea)Rongxin Cai0Xican Li1Chunhou Li2Jiayi Zhu3Jingyuan Zeng4Jianwu Li5Boxu Tang6Zheng Li7Shuqin Liu8Yan Yan9School of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, Waihuang East Road No. 232, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, Waihuang East Road No. 232, Guangzhou 510006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, Waihuang East Road No. 232, Guangzhou 510006, ChinaGuangdong Food Industry Institute Co., Ltd., Guangzhou 511442, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, Waihuang East Road No. 232, Guangzhou 510006, ChinaSchool of Pharmacy and Food Science, Zhuhai College Jilin University, Zhuhai 519040, ChinaSchool of Pharmacy and Food Science, Zhuhai College Jilin University, Zhuhai 519040, ChinaCollege of Information Science and Engineering, Hunan Normal University, Changsha 410006, ChinaSchool of Chinese Herbal Medicine, Guangzhou University of Chinese Medicine, Guangzhou Higher Education Mega Center, Waihuang East Road No. 232, Guangzhou 510006, ChinaSchool of Pharmacy and Food Science, Zhuhai College Jilin University, Zhuhai 519040, China<i>Ampelopsis grossedentata</i> (vine tea) has been used as a detoxifying beverage in China for centuries. To systematically identify its bioactive compounds, the study adopted standards-based ultra-high-performance liquid chromatography coupled with quadrupole/electrostatic field orbitrap high-resolution mass spectrometry (UPLC-Q-Exactive Orbitrap MS) analysis. The analysis was conducted under a negative ion model and the data were collected using the Xcalibur 4.1 software package. Based on comparisons with authentic standards, 36 bioactive compounds were putatively identified by four parameters: retention time, molecular ion peak, MS/MS profile, and characteristic fragments. These bioactive compounds include two chromones (noreugenin and 3,5,7-trihydroxychromone), 15 flavonoids (S-eriodictyol, S-naringenin, luteolin, ampelopsin, taxifolin, myricetin, quercetin, viscidulin I, kaempferol, myricetin 3-<i>O</i>-galactoside, myricitrin, avicularin, quercitrin, isorhamnetin-3-<i>O</i>-<i>β</i>-D-glucoside, and afzelin), four phenolic acids (gallic acid, 3,4-dihydroxy-5-methoxybenzoic acid, syringic acid, and ellagic acid), five tea polyphenols (epigallocatechin, epigallocatechin gallate, gallocatechin gallate, epicatechin gallate, and catechin gallate), three chalcones (phloridzin, phloretin, and naringenin chalcone), one stilbene (polydatin), two lipids (myristic acid and ethyl stearate), one sugar (D-gluconic acid), one amino acid (L-tryptophan), one triterpenoid (oleanolic acid) and one alkaloid (jervine). Notably, the jervine identification is the first report regarding the occurrence of alkaloid in the plant. Two chromones may be the parent skeleton to biosynthesize the flavonoid in <i>A. grossedentata.</i>https://www.mdpi.com/2297-8739/9/11/329<i>Ampelopsis grossedentata</i>polyphenoljervineUPLC-Q-Exactive Orbitrap MSsystematic identificationflavones |
| spellingShingle | Rongxin Cai Xican Li Chunhou Li Jiayi Zhu Jingyuan Zeng Jianwu Li Boxu Tang Zheng Li Shuqin Liu Yan Yan Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea) Separations <i>Ampelopsis grossedentata</i> polyphenol jervine UPLC-Q-Exactive Orbitrap MS systematic identification flavones |
| title | Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea) |
| title_full | Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea) |
| title_fullStr | Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea) |
| title_full_unstemmed | Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea) |
| title_short | Standards-Based UPLC-Q-Exactive Orbitrap MS Systematically Identifies 36 Bioactive Compounds in <i>Ampelopsis grossedentata</i> (Vine Tea) |
| title_sort | standards based uplc q exactive orbitrap ms systematically identifies 36 bioactive compounds in i ampelopsis grossedentata i vine tea |
| topic | <i>Ampelopsis grossedentata</i> polyphenol jervine UPLC-Q-Exactive Orbitrap MS systematic identification flavones |
| url | https://www.mdpi.com/2297-8739/9/11/329 |
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