Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems
Ticks are vectors and reservoirs of many arboviruses pathogenic for humans or domestic animals; in addition, during bloodfeeding they can acquire and harbour pathogenic arboviruses normally transmitted by other arthropods such as mosquitoes. Tick cell and organ cultures provide convenient tools for...
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-09-01
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| Series: | Veterinary Sciences |
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| Online Access: | http://www.mdpi.com/2306-7381/3/4/28 |
| _version_ | 1818944360128970752 |
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| author | Lesley Bell-Sakyi Sabine Weisheit Claudia Rückert Gerald Barry John Fazakerley Rennos Fragkoudis |
| author_facet | Lesley Bell-Sakyi Sabine Weisheit Claudia Rückert Gerald Barry John Fazakerley Rennos Fragkoudis |
| author_sort | Lesley Bell-Sakyi |
| collection | DOAJ |
| description | Ticks are vectors and reservoirs of many arboviruses pathogenic for humans or domestic animals; in addition, during bloodfeeding they can acquire and harbour pathogenic arboviruses normally transmitted by other arthropods such as mosquitoes. Tick cell and organ cultures provide convenient tools for propagation and study of arboviruses, both tick-borne and insect-borne, enabling elucidation of virus-tick cell interaction and yielding insight into the mechanisms behind vector competence and reservoir potential for different arbovirus species. The mosquito-borne zoonotic alphavirus Semliki Forest virus (SFV), which replicates well in tick cells, has been isolated from Rhipicephalus, Hyalomma, and Amblyomma spp. ticks removed from mammalian hosts in East Africa; however nothing is known about any possible role of ticks in SFV epidemiology. Here we present a light and electron microscopic study of SFV infecting cell lines and organ cultures derived from African Rhipicephalus spp. ticks. As well as demonstrating the applicability of these culture systems for studying virus-vector interactions, we provide preliminary evidence to support the hypothesis that SFV is not normally transmitted by ticks because the virus does not infect midgut cells. |
| first_indexed | 2024-12-20T07:41:59Z |
| format | Article |
| id | doaj.art-a67d344895a94e389f93b2cec6883c06 |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-12-20T07:41:59Z |
| publishDate | 2016-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-a67d344895a94e389f93b2cec6883c062022-12-21T19:48:06ZengMDPI AGVeterinary Sciences2306-73812016-09-01342810.3390/vetsci3040028vetsci3040028Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter SystemsLesley Bell-Sakyi0Sabine Weisheit1Claudia Rückert2Gerald Barry3John Fazakerley4Rennos Fragkoudis5The Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UKThe Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UKThe Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UKThe Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UKThe Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UKThe Pirbright Institute, Ash Road, Pirbright, Surrey GU24 0NF, UKTicks are vectors and reservoirs of many arboviruses pathogenic for humans or domestic animals; in addition, during bloodfeeding they can acquire and harbour pathogenic arboviruses normally transmitted by other arthropods such as mosquitoes. Tick cell and organ cultures provide convenient tools for propagation and study of arboviruses, both tick-borne and insect-borne, enabling elucidation of virus-tick cell interaction and yielding insight into the mechanisms behind vector competence and reservoir potential for different arbovirus species. The mosquito-borne zoonotic alphavirus Semliki Forest virus (SFV), which replicates well in tick cells, has been isolated from Rhipicephalus, Hyalomma, and Amblyomma spp. ticks removed from mammalian hosts in East Africa; however nothing is known about any possible role of ticks in SFV epidemiology. Here we present a light and electron microscopic study of SFV infecting cell lines and organ cultures derived from African Rhipicephalus spp. ticks. As well as demonstrating the applicability of these culture systems for studying virus-vector interactions, we provide preliminary evidence to support the hypothesis that SFV is not normally transmitted by ticks because the virus does not infect midgut cells.http://www.mdpi.com/2306-7381/3/4/28tick cell linetick organ culturearbovirusSemliki Forest virusreporter genemicroscopy |
| spellingShingle | Lesley Bell-Sakyi Sabine Weisheit Claudia Rückert Gerald Barry John Fazakerley Rennos Fragkoudis Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems Veterinary Sciences tick cell line tick organ culture arbovirus Semliki Forest virus reporter gene microscopy |
| title | Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems |
| title_full | Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems |
| title_fullStr | Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems |
| title_full_unstemmed | Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems |
| title_short | Microscopic Visualisation of Zoonotic Arbovirus Replication in Tick Cell and Organ Cultures Using Semliki Forest Virus Reporter Systems |
| title_sort | microscopic visualisation of zoonotic arbovirus replication in tick cell and organ cultures using semliki forest virus reporter systems |
| topic | tick cell line tick organ culture arbovirus Semliki Forest virus reporter gene microscopy |
| url | http://www.mdpi.com/2306-7381/3/4/28 |
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