Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments.
It has been shown that modestly increasing plasma membrane cholesterol beyond its physiological set point greatly increases the endoplasmic reticulum and mitochondrial pools, thereby eliciting manifold feedback responses that return cell cholesterol to its resting state. The question arises whether...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4094430?pdf=render |
| _version_ | 1817971342866120704 |
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| author | Yvonne Lange Jin Ye Theodore L Steck |
| author_facet | Yvonne Lange Jin Ye Theodore L Steck |
| author_sort | Yvonne Lange |
| collection | DOAJ |
| description | It has been shown that modestly increasing plasma membrane cholesterol beyond its physiological set point greatly increases the endoplasmic reticulum and mitochondrial pools, thereby eliciting manifold feedback responses that return cell cholesterol to its resting state. The question arises whether this homeostatic mechanism reflects the targeting of cell surface cholesterol to specific intracellular sites or its general equilibration among the organelles. We now show that human fibroblast cholesterol can be increased as much as two-fold from 2-hydroxypropyl-β-cyclodextrin without changing the size of the cell surface pool. Rather, essentially all of the added cholesterol disperses rapidly among cytoplasmic membranes, increasing their overall cholesterol content by as much as five-fold. We conclude that the level of plasma membrane cholesterol is normally at capacity and that even small increments above this physiological set point redistribute essentially entirely to intracellular membranes, perhaps down their chemical activity gradients. |
| first_indexed | 2024-04-13T20:45:34Z |
| format | Article |
| id | doaj.art-a67eb3c421174c3c8613013055580624 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T20:45:34Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a67eb3c421174c3c86130130555806242022-12-22T02:30:43ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0197e9848210.1371/journal.pone.0098482Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments.Yvonne LangeJin YeTheodore L SteckIt has been shown that modestly increasing plasma membrane cholesterol beyond its physiological set point greatly increases the endoplasmic reticulum and mitochondrial pools, thereby eliciting manifold feedback responses that return cell cholesterol to its resting state. The question arises whether this homeostatic mechanism reflects the targeting of cell surface cholesterol to specific intracellular sites or its general equilibration among the organelles. We now show that human fibroblast cholesterol can be increased as much as two-fold from 2-hydroxypropyl-β-cyclodextrin without changing the size of the cell surface pool. Rather, essentially all of the added cholesterol disperses rapidly among cytoplasmic membranes, increasing their overall cholesterol content by as much as five-fold. We conclude that the level of plasma membrane cholesterol is normally at capacity and that even small increments above this physiological set point redistribute essentially entirely to intracellular membranes, perhaps down their chemical activity gradients.http://europepmc.org/articles/PMC4094430?pdf=render |
| spellingShingle | Yvonne Lange Jin Ye Theodore L Steck Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments. PLoS ONE |
| title | Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments. |
| title_full | Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments. |
| title_fullStr | Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments. |
| title_full_unstemmed | Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments. |
| title_short | Essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments. |
| title_sort | essentially all excess fibroblast cholesterol moves from plasma membranes to intracellular compartments |
| url | http://europepmc.org/articles/PMC4094430?pdf=render |
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