Novel 3D organotypic co-culture model of pleura
Pleural mesothelial cells are the predominant cell type in the pleural cavity, but their role in the pathogenesis of pleural diseases needs to be further elucidated. 3D organotypic models are an encouraging approach for an in vivo understanding of molecular disease development. The aim of the presen...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714887/?tool=EBI |
| _version_ | 1811209012330364928 |
|---|---|
| author | Isabella B. Metelmann Sebastian Kraemer Matthias Steinert Stefan Langer Peggy Stock Olga Kurow |
| author_facet | Isabella B. Metelmann Sebastian Kraemer Matthias Steinert Stefan Langer Peggy Stock Olga Kurow |
| author_sort | Isabella B. Metelmann |
| collection | DOAJ |
| description | Pleural mesothelial cells are the predominant cell type in the pleural cavity, but their role in the pathogenesis of pleural diseases needs to be further elucidated. 3D organotypic models are an encouraging approach for an in vivo understanding of molecular disease development. The aim of the present study was to develop a 3D organotypic model of the pleural mesothelium. Specimens of human pleura parietalis were obtained from patients undergoing surgery at the University Hospital Leipzig, Germany. 3D co-culture model of pleura was established from human pleural mesothelial cells and fibroblasts. The model was compared to human pleura tissue by phase-contrast and light microscopy, immunochemistry and -fluorescence as well as solute permeation test. Histological assessment of the 3D co-culture model displayed the presence of both cell types mimicking the morphology of the human pleura. Vimentin and Cytokeratin, PHD1 showed a similar expression pattern in pleural biopsies and 3D model. Expression of Ki-67 indicates the presence of proliferating cells. Tight junctional marker ZO-1 was found localized at contact zones between mesothelial cells. Each of these markers were expressed in both the 3D co-culture model and human biopsies. Permeability of 3D organotypic co-culture model of pleura was found to be higher for 70 kDa-Dextran and no significant difference was seen in the permeability for small dextran (4 kDa). In summary, the presented 3D organoid of pleura functions as a robust assay for pleural research serving as a precise reproduction of the in vivo morphology and microenvironment. |
| first_indexed | 2024-04-12T04:31:30Z |
| format | Article |
| id | doaj.art-a67f6f2d0ed540a98d26132ad4a33fa9 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T04:31:30Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a67f6f2d0ed540a98d26132ad4a33fa92022-12-22T03:47:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712Novel 3D organotypic co-culture model of pleuraIsabella B. MetelmannSebastian KraemerMatthias SteinertStefan LangerPeggy StockOlga KurowPleural mesothelial cells are the predominant cell type in the pleural cavity, but their role in the pathogenesis of pleural diseases needs to be further elucidated. 3D organotypic models are an encouraging approach for an in vivo understanding of molecular disease development. The aim of the present study was to develop a 3D organotypic model of the pleural mesothelium. Specimens of human pleura parietalis were obtained from patients undergoing surgery at the University Hospital Leipzig, Germany. 3D co-culture model of pleura was established from human pleural mesothelial cells and fibroblasts. The model was compared to human pleura tissue by phase-contrast and light microscopy, immunochemistry and -fluorescence as well as solute permeation test. Histological assessment of the 3D co-culture model displayed the presence of both cell types mimicking the morphology of the human pleura. Vimentin and Cytokeratin, PHD1 showed a similar expression pattern in pleural biopsies and 3D model. Expression of Ki-67 indicates the presence of proliferating cells. Tight junctional marker ZO-1 was found localized at contact zones between mesothelial cells. Each of these markers were expressed in both the 3D co-culture model and human biopsies. Permeability of 3D organotypic co-culture model of pleura was found to be higher for 70 kDa-Dextran and no significant difference was seen in the permeability for small dextran (4 kDa). In summary, the presented 3D organoid of pleura functions as a robust assay for pleural research serving as a precise reproduction of the in vivo morphology and microenvironment.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714887/?tool=EBI |
| spellingShingle | Isabella B. Metelmann Sebastian Kraemer Matthias Steinert Stefan Langer Peggy Stock Olga Kurow Novel 3D organotypic co-culture model of pleura PLoS ONE |
| title | Novel 3D organotypic co-culture model of pleura |
| title_full | Novel 3D organotypic co-culture model of pleura |
| title_fullStr | Novel 3D organotypic co-culture model of pleura |
| title_full_unstemmed | Novel 3D organotypic co-culture model of pleura |
| title_short | Novel 3D organotypic co-culture model of pleura |
| title_sort | novel 3d organotypic co culture model of pleura |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714887/?tool=EBI |
| work_keys_str_mv | AT isabellabmetelmann novel3dorganotypiccoculturemodelofpleura AT sebastiankraemer novel3dorganotypiccoculturemodelofpleura AT matthiassteinert novel3dorganotypiccoculturemodelofpleura AT stefanlanger novel3dorganotypiccoculturemodelofpleura AT peggystock novel3dorganotypiccoculturemodelofpleura AT olgakurow novel3dorganotypiccoculturemodelofpleura |