Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i>
<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a major foodborne pathogen. Rapid and specific detection is crucial for controlling staphylococcal food poisoning. This study reported a <i>Staphylococcus</i> phage named LSA2302 showing great potential for applic...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/8/2098 |
| _version_ | 1797583711845220352 |
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| author | Ruining Li Zhiwei Li Chenxi Huang Yifeng Ding Jia Wang Xiaohong Wang |
| author_facet | Ruining Li Zhiwei Li Chenxi Huang Yifeng Ding Jia Wang Xiaohong Wang |
| author_sort | Ruining Li |
| collection | DOAJ |
| description | <i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a major foodborne pathogen. Rapid and specific detection is crucial for controlling staphylococcal food poisoning. This study reported a <i>Staphylococcus</i> phage named LSA2302 showing great potential for applications in the rapid detection of <i>S. aureus</i>. Its biological characteristics were identified, including growth properties and stability under different pH and temperature conditions. The genomic analysis revealed that the phage has no genes associated with pathogenicity or drug resistance. Then, the phage-functionalized magnetic beads (pMB), serving as a biological recognition element, were integrated with ATP bioluminescence assays to establish a biosensing method for <i>S. aureus</i> detection. The pMB enrichment brought high specificity and a tenfold increase in analytical sensitivity during detection. The whole detection process could be completed within 30 min, with a broad linear range of 1 × 10<sup>4</sup> to 1 × 10<sup>8</sup> CFU/mL and a limit of detection (LOD) of 2.43 × 10<sup>3</sup> CFU/mL. After a 2 h pre-cultivation, this method is capable of detecting bacteria as low as 1 CFU/mL. The recoveries of <i>S. aureus</i> in spiked skim milk and chicken samples were 81.07% to 99.17% and 86.98% to 104.62%, respectively. Our results indicated that phage-based biosensing can contribute to the detection of target pathogens in foods. |
| first_indexed | 2024-03-10T23:42:54Z |
| format | Article |
| id | doaj.art-a682e2be6e9c488cb58eb4724cb6f828 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T23:42:54Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a682e2be6e9c488cb58eb4724cb6f8282023-11-19T02:19:13ZengMDPI AGMicroorganisms2076-26072023-08-01118209810.3390/microorganisms11082098Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i>Ruining Li0Zhiwei Li1Chenxi Huang2Yifeng Ding3Jia Wang4Xiaohong Wang5College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, ChinaCollege of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China<i>Staphylococcus aureus</i> (<i>S. aureus</i>) is a major foodborne pathogen. Rapid and specific detection is crucial for controlling staphylococcal food poisoning. This study reported a <i>Staphylococcus</i> phage named LSA2302 showing great potential for applications in the rapid detection of <i>S. aureus</i>. Its biological characteristics were identified, including growth properties and stability under different pH and temperature conditions. The genomic analysis revealed that the phage has no genes associated with pathogenicity or drug resistance. Then, the phage-functionalized magnetic beads (pMB), serving as a biological recognition element, were integrated with ATP bioluminescence assays to establish a biosensing method for <i>S. aureus</i> detection. The pMB enrichment brought high specificity and a tenfold increase in analytical sensitivity during detection. The whole detection process could be completed within 30 min, with a broad linear range of 1 × 10<sup>4</sup> to 1 × 10<sup>8</sup> CFU/mL and a limit of detection (LOD) of 2.43 × 10<sup>3</sup> CFU/mL. After a 2 h pre-cultivation, this method is capable of detecting bacteria as low as 1 CFU/mL. The recoveries of <i>S. aureus</i> in spiked skim milk and chicken samples were 81.07% to 99.17% and 86.98% to 104.62%, respectively. Our results indicated that phage-based biosensing can contribute to the detection of target pathogens in foods.https://www.mdpi.com/2076-2607/11/8/2098<i>Staphylococcus aureus</i><i>Staphylococcus</i> phagemagnetic beadsATP bioluminescence assay |
| spellingShingle | Ruining Li Zhiwei Li Chenxi Huang Yifeng Ding Jia Wang Xiaohong Wang Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i> Microorganisms <i>Staphylococcus aureus</i> <i>Staphylococcus</i> phage magnetic beads ATP bioluminescence assay |
| title | Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i> |
| title_full | Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i> |
| title_fullStr | Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i> |
| title_short | Phage-Based Biosensing for Rapid and Specific Detection of <i>Staphylococcus aureus</i> |
| title_sort | phage based biosensing for rapid and specific detection of i staphylococcus aureus i |
| topic | <i>Staphylococcus aureus</i> <i>Staphylococcus</i> phage magnetic beads ATP bioluminescence assay |
| url | https://www.mdpi.com/2076-2607/11/8/2098 |
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