miRNA regulation in the early development of barley seed
<p>Abstract</p> <p>Background</p> <p>During the early stages of seed development many genes are under dynamic regulation to ensure the proper differentiation and establishment of the tissue that will constitute the mature grain. To investigate how miRNA regulation contr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2229/12/120 |
| _version_ | 1818119864377671680 |
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| author | Curaba Julien Spriggs Andrew Taylor Jen Li Zhongyi Helliwell Chris |
| author_facet | Curaba Julien Spriggs Andrew Taylor Jen Li Zhongyi Helliwell Chris |
| author_sort | Curaba Julien |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>During the early stages of seed development many genes are under dynamic regulation to ensure the proper differentiation and establishment of the tissue that will constitute the mature grain. To investigate how miRNA regulation contributes to this process in barley, a combination of small RNA and mRNA degradome analyses were used to identify miRNAs and their targets.</p> <p>Results</p> <p>Our analysis identified 84 known miRNAs and 7 new miRNAs together with 96 putative miRNA target genes regulated through a slicing mechanism in grain tissues during the first 15 days post anthesis. We also identified many potential miRNAs including several belonging to known miRNA families. Our data gave us evidence for an increase in miRNA-mediated regulation during the transition between pre-storage and storage phases. Potential miRNA targets were found in various signalling pathways including components of four phytohormone pathways (ABA, GA, auxin, ethylene) and the defence response to powdery mildew infection. Among the putative miRNA targets we identified were two essential genes controlling the GA response, a <it>GA3oxidase1</it> and a homolog of the receptor <it>GID1</it>, and a homolog of the ACC oxidase which catalyses the last step of ethylene biosynthesis. We found that two <it>MLA</it> genes are potentially miRNA regulated, establishing a direct link between miRNAs and the R gene response.</p> <p>Conclusion</p> <p>Our dataset provides a useful source of information on miRNA regulation during the early development of cereal grains and our analysis suggests that miRNAs contribute to the control of development of the cereal grain, notably through the regulation of phytohormone response pathways.</p> |
| first_indexed | 2024-12-11T05:16:58Z |
| format | Article |
| id | doaj.art-a684c415bc2748219ab106a1c90eada0 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-11T05:16:58Z |
| publishDate | 2012-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-a684c415bc2748219ab106a1c90eada02022-12-22T01:19:48ZengBMCBMC Plant Biology1471-22292012-07-0112112010.1186/1471-2229-12-120miRNA regulation in the early development of barley seedCuraba JulienSpriggs AndrewTaylor JenLi ZhongyiHelliwell Chris<p>Abstract</p> <p>Background</p> <p>During the early stages of seed development many genes are under dynamic regulation to ensure the proper differentiation and establishment of the tissue that will constitute the mature grain. To investigate how miRNA regulation contributes to this process in barley, a combination of small RNA and mRNA degradome analyses were used to identify miRNAs and their targets.</p> <p>Results</p> <p>Our analysis identified 84 known miRNAs and 7 new miRNAs together with 96 putative miRNA target genes regulated through a slicing mechanism in grain tissues during the first 15 days post anthesis. We also identified many potential miRNAs including several belonging to known miRNA families. Our data gave us evidence for an increase in miRNA-mediated regulation during the transition between pre-storage and storage phases. Potential miRNA targets were found in various signalling pathways including components of four phytohormone pathways (ABA, GA, auxin, ethylene) and the defence response to powdery mildew infection. Among the putative miRNA targets we identified were two essential genes controlling the GA response, a <it>GA3oxidase1</it> and a homolog of the receptor <it>GID1</it>, and a homolog of the ACC oxidase which catalyses the last step of ethylene biosynthesis. We found that two <it>MLA</it> genes are potentially miRNA regulated, establishing a direct link between miRNAs and the R gene response.</p> <p>Conclusion</p> <p>Our dataset provides a useful source of information on miRNA regulation during the early development of cereal grains and our analysis suggests that miRNAs contribute to the control of development of the cereal grain, notably through the regulation of phytohormone response pathways.</p>http://www.biomedcentral.com/1471-2229/12/120microRNABarleyGrain developmentPlant hormonesDisease resistanceSmall RNA sequencingmRNA degradomePARE |
| spellingShingle | Curaba Julien Spriggs Andrew Taylor Jen Li Zhongyi Helliwell Chris miRNA regulation in the early development of barley seed BMC Plant Biology microRNA Barley Grain development Plant hormones Disease resistance Small RNA sequencing mRNA degradome PARE |
| title | miRNA regulation in the early development of barley seed |
| title_full | miRNA regulation in the early development of barley seed |
| title_fullStr | miRNA regulation in the early development of barley seed |
| title_full_unstemmed | miRNA regulation in the early development of barley seed |
| title_short | miRNA regulation in the early development of barley seed |
| title_sort | mirna regulation in the early development of barley seed |
| topic | microRNA Barley Grain development Plant hormones Disease resistance Small RNA sequencing mRNA degradome PARE |
| url | http://www.biomedcentral.com/1471-2229/12/120 |
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