Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana
ABSTRACT: In recent years, compounds with biological properties produced by plants have received attention as an alternative to control microorganisms. Essential oils extracted from green leaves of Eucalyptus sp. have been demonstrated to have antimicrobial activities, but so far there are no report...
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| Format: | Article |
| Language: | English |
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Instituto Biológico
2019-01-01
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| Series: | Arquivos do Instituto Biológico |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100201&lng=en&tlng=en |
| _version_ | 1818889147237007360 |
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| author | Marcos Saldanha Correa Joseli Schwambach Michele Bertoni Mann Jeverson Frazzon Ana Paula Guedes Frazzon |
| author_facet | Marcos Saldanha Correa Joseli Schwambach Michele Bertoni Mann Jeverson Frazzon Ana Paula Guedes Frazzon |
| author_sort | Marcos Saldanha Correa |
| collection | DOAJ |
| description | ABSTRACT: In recent years, compounds with biological properties produced by plants have received attention as an alternative to control microorganisms. Essential oils extracted from green leaves of Eucalyptus sp. have been demonstrated to have antimicrobial activities, but so far there are no reports of antimicrobial activity of essential oils extracted from dried leaves of Eucalyptus staigeriana. So, the objectives of this study were to determine the chemical composition of the essential oils obtained from dried leaves of E. staigeriana (EOdlES) and to evaluate in vitro antimicrobial and antibiofilm activities of EOdlES against gram-positive and gram-negative, resistance and multiresistant Enterococcus faecalis isolated from food and clinical samples. The characterization of EOdlES was performed by gas chromatography-mass spectrometry (GC/MS). For this study, 26 bacterial strains were used, which included 11 reference strains and 15 antibiotic resistant and multiresistant E. faecalis strains. Antimicrobial activities of EOdlES against gram-positive and gram-negative were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) value was evaluated by a microbroth dilution technique. The antibiofilm effects were assessed by microtiter plate method. As a result, 21 compounds were identified, being oxygenated monoterpenes (69.58%) the major chemical family. EOdlES showed only antimicrobial activity against gram-positive strains. E. faecalis resistant and multiresistant strains show the lowest MIC (3.12 to 6.25%), when compared with reference E. faecalis strain. EOdlES has the ability to inhibit the biofilm formation, but little or none ability to inhibit the preformed biofilm. This study demonstrates that EOdlES is a promising alternative to control important foodborne and clinic gram-positive resistant bacteria. |
| first_indexed | 2024-12-19T17:04:24Z |
| format | Article |
| id | doaj.art-a687c00808b8422986d0d8d243f34e20 |
| institution | Directory Open Access Journal |
| issn | 1808-1657 |
| language | English |
| last_indexed | 2024-12-19T17:04:24Z |
| publishDate | 2019-01-01 |
| publisher | Instituto Biológico |
| record_format | Article |
| series | Arquivos do Instituto Biológico |
| spelling | doaj.art-a687c00808b8422986d0d8d243f34e202022-12-21T20:13:13ZengInstituto BiológicoArquivos do Instituto Biológico1808-16572019-01-0186010.1590/1808-1657000202018S1808-16572019000100201Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigerianaMarcos Saldanha CorreaJoseli SchwambachMichele Bertoni MannJeverson FrazzonAna Paula Guedes FrazzonABSTRACT: In recent years, compounds with biological properties produced by plants have received attention as an alternative to control microorganisms. Essential oils extracted from green leaves of Eucalyptus sp. have been demonstrated to have antimicrobial activities, but so far there are no reports of antimicrobial activity of essential oils extracted from dried leaves of Eucalyptus staigeriana. So, the objectives of this study were to determine the chemical composition of the essential oils obtained from dried leaves of E. staigeriana (EOdlES) and to evaluate in vitro antimicrobial and antibiofilm activities of EOdlES against gram-positive and gram-negative, resistance and multiresistant Enterococcus faecalis isolated from food and clinical samples. The characterization of EOdlES was performed by gas chromatography-mass spectrometry (GC/MS). For this study, 26 bacterial strains were used, which included 11 reference strains and 15 antibiotic resistant and multiresistant E. faecalis strains. Antimicrobial activities of EOdlES against gram-positive and gram-negative were determined using the disc diffusion method. The minimum inhibitory concentration (MIC) value was evaluated by a microbroth dilution technique. The antibiofilm effects were assessed by microtiter plate method. As a result, 21 compounds were identified, being oxygenated monoterpenes (69.58%) the major chemical family. EOdlES showed only antimicrobial activity against gram-positive strains. E. faecalis resistant and multiresistant strains show the lowest MIC (3.12 to 6.25%), when compared with reference E. faecalis strain. EOdlES has the ability to inhibit the biofilm formation, but little or none ability to inhibit the preformed biofilm. This study demonstrates that EOdlES is a promising alternative to control important foodborne and clinic gram-positive resistant bacteria.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100201&lng=en&tlng=enatividade antimicrobianaatividade antibiofilmeóleos essenciaisfolhas secascepas resistentes a antibióticosEucalyptus staigeriana |
| spellingShingle | Marcos Saldanha Correa Joseli Schwambach Michele Bertoni Mann Jeverson Frazzon Ana Paula Guedes Frazzon Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana Arquivos do Instituto Biológico atividade antimicrobiana atividade antibiofilme óleos essenciais folhas secas cepas resistentes a antibióticos Eucalyptus staigeriana |
| title | Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana |
| title_full | Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana |
| title_fullStr | Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana |
| title_full_unstemmed | Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana |
| title_short | Antimicrobial and antibiofilm activity of the essential oil from dried leaves of Eucalyptus staigeriana |
| title_sort | antimicrobial and antibiofilm activity of the essential oil from dried leaves of eucalyptus staigeriana |
| topic | atividade antimicrobiana atividade antibiofilme óleos essenciais folhas secas cepas resistentes a antibióticos Eucalyptus staigeriana |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1808-16572019000100201&lng=en&tlng=en |
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