Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage Formation
Norisoboldine (NOR), the major isoquinoline alkaloid constituent of a Chinese traditional medicine Radix Linderae, has been demonstrated to inhibit osteoclast differentiation and improve arthritis. The aim of this study is to examine the effect of NOR on bone fracture healing and the underlying mech...
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MDPI AG
2023-07-01
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| author | Wenliang Yan Meng Shen Kainong Sun Shiming Li Jingyuan Miao Jun Wang Jiayang Xu Pengcheng Wen Qian Zhang |
| author_facet | Wenliang Yan Meng Shen Kainong Sun Shiming Li Jingyuan Miao Jun Wang Jiayang Xu Pengcheng Wen Qian Zhang |
| author_sort | Wenliang Yan |
| collection | DOAJ |
| description | Norisoboldine (NOR), the major isoquinoline alkaloid constituent of a Chinese traditional medicine Radix Linderae, has been demonstrated to inhibit osteoclast differentiation and improve arthritis. The aim of this study is to examine the effect of NOR on bone fracture healing and the underlying mechanisms correlated with bone marrow stromal cells (BMSCs) differentiation to chondrocytes. Our results showed that NOR inhibits the tibia fracture healing process by suppressing cartilage formation, which leads to less endochondral ossification, indicated by less osterix and collage I signaling at the fracture site. Moreover, NOR significantly reduced the differentiation of primary BMSCs to chondrocytes in vitro by reducing the bone morphogenetic protein 2 (BMP2) signaling. These findings imply that NOR negatively regulates the healing of the tibial midshaft fracture, which might delay the union of the fractures and should be noticed when used in other treatments. |
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| language | English |
| last_indexed | 2024-03-11T01:16:51Z |
| publishDate | 2023-07-01 |
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| series | Biomedicines |
| spelling | doaj.art-a688f1450009401894cc649f14b64a202023-11-18T18:28:28ZengMDPI AGBiomedicines2227-90592023-07-01117203110.3390/biomedicines11072031Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage FormationWenliang Yan0Meng Shen1Kainong Sun2Shiming Li3Jingyuan Miao4Jun Wang5Jiayang Xu6Pengcheng Wen7Qian Zhang8College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaDepartment of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaDepartment of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaDepartment of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaDepartment of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaDepartment of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaCollege of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, ChinaDepartment of Nutrition and Health, China Agricultural University, Beijing 100193, ChinaNorisoboldine (NOR), the major isoquinoline alkaloid constituent of a Chinese traditional medicine Radix Linderae, has been demonstrated to inhibit osteoclast differentiation and improve arthritis. The aim of this study is to examine the effect of NOR on bone fracture healing and the underlying mechanisms correlated with bone marrow stromal cells (BMSCs) differentiation to chondrocytes. Our results showed that NOR inhibits the tibia fracture healing process by suppressing cartilage formation, which leads to less endochondral ossification, indicated by less osterix and collage I signaling at the fracture site. Moreover, NOR significantly reduced the differentiation of primary BMSCs to chondrocytes in vitro by reducing the bone morphogenetic protein 2 (BMP2) signaling. These findings imply that NOR negatively regulates the healing of the tibial midshaft fracture, which might delay the union of the fractures and should be noticed when used in other treatments.https://www.mdpi.com/2227-9059/11/7/2031norisoboldinefracture healingBMP2endochondral ossification |
| spellingShingle | Wenliang Yan Meng Shen Kainong Sun Shiming Li Jingyuan Miao Jun Wang Jiayang Xu Pengcheng Wen Qian Zhang Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage Formation Biomedicines norisoboldine fracture healing BMP2 endochondral ossification |
| title | Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage Formation |
| title_full | Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage Formation |
| title_fullStr | Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage Formation |
| title_full_unstemmed | Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage Formation |
| title_short | Norisoboldine, a Natural Isoquinoline Alkaloid, Inhibits Diaphyseal Fracture Healing in Mice by Alleviating Cartilage Formation |
| title_sort | norisoboldine a natural isoquinoline alkaloid inhibits diaphyseal fracture healing in mice by alleviating cartilage formation |
| topic | norisoboldine fracture healing BMP2 endochondral ossification |
| url | https://www.mdpi.com/2227-9059/11/7/2031 |
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