X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets
X-rays can induce morphological as well as functional changes in cells. Platelets are anuclear cellular fragments originating from megakaryocytes and are the major regulators in hemostasis and thrombosis. Platelet products are irradiated to avoid medical complications associated with platelet transf...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/45/7/380 |
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| author | Muhammad Shoaib Khan Chunliang Liu Fanbi Meng Mengnan Yang Kangxi Zhou Renping Hu Xuexiang Wang Kesheng Dai |
| author_facet | Muhammad Shoaib Khan Chunliang Liu Fanbi Meng Mengnan Yang Kangxi Zhou Renping Hu Xuexiang Wang Kesheng Dai |
| author_sort | Muhammad Shoaib Khan |
| collection | DOAJ |
| description | X-rays can induce morphological as well as functional changes in cells. Platelets are anuclear cellular fragments originating from megakaryocytes and are the major regulators in hemostasis and thrombosis. Platelet products are irradiated to avoid medical complications associated with platelet transfusion. So far, gamma, UV, and laser radiation have been used for this purpose. However, scientists are divided about the effects of radiation on platelet quality. The present study was designed to explore the possible effects of X-rays in washed human platelets and understand the molecular mechanism behind them. In the present study, we exposed washed human platelets to 10 or 30 Gy X-rays at 0.25 Gy/min. Flow cytometry, aggregometry, and western blot were performed to investigate the effect of X-rays on platelet degranulation, integrin activation, platelet aggregation, and apoptosis. It was found that X-rays immediately induced granular secretions with no effect on GP IIb/IIIa activation. Not surprisingly, due to granule secretions in irradiated platelets, platelet aggregation was significantly reduced. In contrast to granular secretions and platelet aggregation, X-rays induced mitochondrial transmembrane potential depolarization in a time-dependent manner to induce apoptosis and activated protein kinase C (PKC) signaling. This study revealed and explained the molecular mechanism activated by X-rays in washed human platelets. Here we also introduced Gö 6983, a PKC inhibitor, as an agent that counteracts X-ray-induced changes and maintains the integrity of platelets. |
| first_indexed | 2024-03-11T01:10:57Z |
| format | Article |
| id | doaj.art-a6893681cc2d4c34ac7725b3562fe159 |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-03-11T01:10:57Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Current Issues in Molecular Biology |
| spelling | doaj.art-a6893681cc2d4c34ac7725b3562fe1592023-11-18T18:51:26ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452023-07-014576024603910.3390/cimb45070380X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human PlateletsMuhammad Shoaib Khan0Chunliang Liu1Fanbi Meng2Mengnan Yang3Kangxi Zhou4Renping Hu5Xuexiang Wang6Kesheng Dai7Jiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaJiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaJiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaJiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaJiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaJiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaJiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaJiangsu Institute of Hematology, The First Affiliated Hospital and Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Protection, Medical College, Soochow University, Key Laboratory of Thrombosis and Hemostasis, Ministry of Health, Suzhou 215006, ChinaX-rays can induce morphological as well as functional changes in cells. Platelets are anuclear cellular fragments originating from megakaryocytes and are the major regulators in hemostasis and thrombosis. Platelet products are irradiated to avoid medical complications associated with platelet transfusion. So far, gamma, UV, and laser radiation have been used for this purpose. However, scientists are divided about the effects of radiation on platelet quality. The present study was designed to explore the possible effects of X-rays in washed human platelets and understand the molecular mechanism behind them. In the present study, we exposed washed human platelets to 10 or 30 Gy X-rays at 0.25 Gy/min. Flow cytometry, aggregometry, and western blot were performed to investigate the effect of X-rays on platelet degranulation, integrin activation, platelet aggregation, and apoptosis. It was found that X-rays immediately induced granular secretions with no effect on GP IIb/IIIa activation. Not surprisingly, due to granule secretions in irradiated platelets, platelet aggregation was significantly reduced. In contrast to granular secretions and platelet aggregation, X-rays induced mitochondrial transmembrane potential depolarization in a time-dependent manner to induce apoptosis and activated protein kinase C (PKC) signaling. This study revealed and explained the molecular mechanism activated by X-rays in washed human platelets. Here we also introduced Gö 6983, a PKC inhibitor, as an agent that counteracts X-ray-induced changes and maintains the integrity of platelets.https://www.mdpi.com/1467-3045/45/7/380X-raysplateletsgranular secretionsapoptosisPKC signaling |
| spellingShingle | Muhammad Shoaib Khan Chunliang Liu Fanbi Meng Mengnan Yang Kangxi Zhou Renping Hu Xuexiang Wang Kesheng Dai X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets Current Issues in Molecular Biology X-rays platelets granular secretions apoptosis PKC signaling |
| title | X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets |
| title_full | X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets |
| title_fullStr | X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets |
| title_full_unstemmed | X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets |
| title_short | X-rays Stimulate Granular Secretions and Activate Protein Kinase C Signaling in Human Platelets |
| title_sort | x rays stimulate granular secretions and activate protein kinase c signaling in human platelets |
| topic | X-rays platelets granular secretions apoptosis PKC signaling |
| url | https://www.mdpi.com/1467-3045/45/7/380 |
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