High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China.
In this study Illumina MiSeq was performed to investigate microbial diversity in soil, leaves, grape, grape juice and wine. A total of 1,043,102 fungal Internal Transcribed Spacer (ITS) reads and 2,422,188 high quality bacterial 16S rDNA sequences were used for taxonomic classification, revealed fiv...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5863948?pdf=render |
| _version_ | 1818691694679293952 |
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| author | Yu-Jie Wei Yun Wu Yin-Zhuo Yan Wan Zou Jie Xue Wen-Rui Ma Wei Wang Ge Tian Li-Ye Wang |
| author_facet | Yu-Jie Wei Yun Wu Yin-Zhuo Yan Wan Zou Jie Xue Wen-Rui Ma Wei Wang Ge Tian Li-Ye Wang |
| author_sort | Yu-Jie Wei |
| collection | DOAJ |
| description | In this study Illumina MiSeq was performed to investigate microbial diversity in soil, leaves, grape, grape juice and wine. A total of 1,043,102 fungal Internal Transcribed Spacer (ITS) reads and 2,422,188 high quality bacterial 16S rDNA sequences were used for taxonomic classification, revealed five fungal and eight bacterial phyla. At the genus level, the dominant fungi were Ascomycota, Sordariales, Tetracladium and Geomyces in soil, Aureobasidium and Pleosporaceae in grapes leaves, Aureobasidium in grape and grape juice. The dominant bacteria were Kaistobacter, Arthrobacter, Skermanella and Sphingomonas in soil, Pseudomonas, Acinetobacter and Kaistobacter in grape and grapes leaves, and Oenococcus in grape juice and wine. Principal coordinate analysis showed structural separation between the composition of fungi and bacteria in all samples. This is the first study to understand microbiome population in soil, grape, grapes leaves, grape juice and wine in Xinjiang through High-throughput Sequencing and identify microorganisms like Saccharomyces cerevisiae and Oenococcus spp. that may contribute to the quality and flavor of wine. |
| first_indexed | 2024-12-17T12:45:58Z |
| format | Article |
| id | doaj.art-a6899753007f4a009c6a63722b856065 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T12:45:58Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a6899753007f4a009c6a63722b8560652022-12-21T21:47:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01133e019309710.1371/journal.pone.0193097High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China.Yu-Jie WeiYun WuYin-Zhuo YanWan ZouJie XueWen-Rui MaWei WangGe TianLi-Ye WangIn this study Illumina MiSeq was performed to investigate microbial diversity in soil, leaves, grape, grape juice and wine. A total of 1,043,102 fungal Internal Transcribed Spacer (ITS) reads and 2,422,188 high quality bacterial 16S rDNA sequences were used for taxonomic classification, revealed five fungal and eight bacterial phyla. At the genus level, the dominant fungi were Ascomycota, Sordariales, Tetracladium and Geomyces in soil, Aureobasidium and Pleosporaceae in grapes leaves, Aureobasidium in grape and grape juice. The dominant bacteria were Kaistobacter, Arthrobacter, Skermanella and Sphingomonas in soil, Pseudomonas, Acinetobacter and Kaistobacter in grape and grapes leaves, and Oenococcus in grape juice and wine. Principal coordinate analysis showed structural separation between the composition of fungi and bacteria in all samples. This is the first study to understand microbiome population in soil, grape, grapes leaves, grape juice and wine in Xinjiang through High-throughput Sequencing and identify microorganisms like Saccharomyces cerevisiae and Oenococcus spp. that may contribute to the quality and flavor of wine.http://europepmc.org/articles/PMC5863948?pdf=render |
| spellingShingle | Yu-Jie Wei Yun Wu Yin-Zhuo Yan Wan Zou Jie Xue Wen-Rui Ma Wei Wang Ge Tian Li-Ye Wang High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China. PLoS ONE |
| title | High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China. |
| title_full | High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China. |
| title_fullStr | High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China. |
| title_full_unstemmed | High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China. |
| title_short | High-throughput sequencing of microbial community diversity in soil, grapes, leaves, grape juice and wine of grapevine from China. |
| title_sort | high throughput sequencing of microbial community diversity in soil grapes leaves grape juice and wine of grapevine from china |
| url | http://europepmc.org/articles/PMC5863948?pdf=render |
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