Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia
A 2-year prospective study carried out on ventilator-associated pneumonia (VAP) patients in the intensive care unit at a tertiary care hospital, Hail, Kingdom of Saudi Arabia (KSA), revealed a high prevalence of extremely drug-resistant (XDR) <i>Acinetobacter baumannii</i>. About a 9% in...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2227-9032/10/11/2210 |
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| author | Mohd Saleem Azharuddin Sajid Syed Khaja Ashfaque Hossain Fahaad Alenazi Kamaleldin B. Said Soha Abdallah Moursi Homoud Abdulmohsin Almalaq Hamza Mohamed Ehab Rakha |
| author_facet | Mohd Saleem Azharuddin Sajid Syed Khaja Ashfaque Hossain Fahaad Alenazi Kamaleldin B. Said Soha Abdallah Moursi Homoud Abdulmohsin Almalaq Hamza Mohamed Ehab Rakha |
| author_sort | Mohd Saleem |
| collection | DOAJ |
| description | A 2-year prospective study carried out on ventilator-associated pneumonia (VAP) patients in the intensive care unit at a tertiary care hospital, Hail, Kingdom of Saudi Arabia (KSA), revealed a high prevalence of extremely drug-resistant (XDR) <i>Acinetobacter baumannii</i>. About a 9% increase in the incidence rate of <i>A. baumannii</i> occurred in the VAP patients between 2019 and 2020 (21.4% to 30.7%). In 2019, the isolates were positive for IMP-1 and VIM-2 (31.1% and 25.7%, respectively) as detected by PCR. In comparison, a higher proportion of isolates produced NDM-1 in 2020. Here, we observed a high proportion of resistant ICU isolates towards the most common antibiotics in use. Colistin sensitivity dropped to 91.4% in the year 2020 as compared to 2019 (100%). Thus, the finding of this study has a highly significant clinical implementation in the clinical management strategies for VAP patients. Furthermore, strict implementation of antibiotic stewardship policies, regular surveillance programs for antimicrobial resistance monitoring, and screening for genes encoding drug resistance phenotypes have become imperative. |
| first_indexed | 2024-03-09T19:02:48Z |
| format | Article |
| id | doaj.art-a68cf1610c574e49b17560f725a4f717 |
| institution | Directory Open Access Journal |
| issn | 2227-9032 |
| language | English |
| last_indexed | 2024-03-09T19:02:48Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Healthcare |
| spelling | doaj.art-a68cf1610c574e49b17560f725a4f7172023-11-24T04:51:35ZengMDPI AGHealthcare2227-90322022-11-011011221010.3390/healthcare10112210Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated PneumoniaMohd Saleem0Azharuddin Sajid Syed Khaja1Ashfaque Hossain2Fahaad Alenazi3Kamaleldin B. Said4Soha Abdallah Moursi5Homoud Abdulmohsin Almalaq6Hamza Mohamed7Ehab Rakha8Department of Pathology, College of Medicine, University of Hail, Hail 55476, Saudi ArabiaDepartment of Pathology, College of Medicine, University of Hail, Hail 55476, Saudi ArabiaDepartment of Medical Microbiology and Immunology, RAK Medical and Health Sciences University, Ras Al Khaimah P.O. Box 11172, United Arab EmiratesDepartment of Pharmacology, College of Medicine, University of Hail, Hail 55476, Saudi ArabiaDepartment of Pathology, College of Medicine, University of Hail, Hail 55476, Saudi ArabiaDepartment of Pathology, College of Medicine, University of Hail, Hail 55476, Saudi ArabiaHail Health Cluster, King Khalid Hospital, College of Pharmacy, King Saud University, Riyadh 55421, Saudi ArabiaDepartment of Anatomy, College of Medicine, University of Hail, Hail 55476, Saudi ArabiaLaboratory Department, King Khalid Hospital, Hail 55421, Saudi ArabiaA 2-year prospective study carried out on ventilator-associated pneumonia (VAP) patients in the intensive care unit at a tertiary care hospital, Hail, Kingdom of Saudi Arabia (KSA), revealed a high prevalence of extremely drug-resistant (XDR) <i>Acinetobacter baumannii</i>. About a 9% increase in the incidence rate of <i>A. baumannii</i> occurred in the VAP patients between 2019 and 2020 (21.4% to 30.7%). In 2019, the isolates were positive for IMP-1 and VIM-2 (31.1% and 25.7%, respectively) as detected by PCR. In comparison, a higher proportion of isolates produced NDM-1 in 2020. Here, we observed a high proportion of resistant ICU isolates towards the most common antibiotics in use. Colistin sensitivity dropped to 91.4% in the year 2020 as compared to 2019 (100%). Thus, the finding of this study has a highly significant clinical implementation in the clinical management strategies for VAP patients. Furthermore, strict implementation of antibiotic stewardship policies, regular surveillance programs for antimicrobial resistance monitoring, and screening for genes encoding drug resistance phenotypes have become imperative.https://www.mdpi.com/2227-9032/10/11/2210<i>Acinetobacter baumannii</i>XDRIMP-1VIM-2NDM-1VAP |
| spellingShingle | Mohd Saleem Azharuddin Sajid Syed Khaja Ashfaque Hossain Fahaad Alenazi Kamaleldin B. Said Soha Abdallah Moursi Homoud Abdulmohsin Almalaq Hamza Mohamed Ehab Rakha Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia Healthcare <i>Acinetobacter baumannii</i> XDR IMP-1 VIM-2 NDM-1 VAP |
| title | Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia |
| title_full | Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia |
| title_fullStr | Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia |
| title_full_unstemmed | Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia |
| title_short | Molecular Characterization and Antibiogram of <i>Acinetobacter baumannii</i> Clinical Isolates Recovered from the Patients with Ventilator-Associated Pneumonia |
| title_sort | molecular characterization and antibiogram of i acinetobacter baumannii i clinical isolates recovered from the patients with ventilator associated pneumonia |
| topic | <i>Acinetobacter baumannii</i> XDR IMP-1 VIM-2 NDM-1 VAP |
| url | https://www.mdpi.com/2227-9032/10/11/2210 |
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