Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysis
AbstractBackground Catheter-related infection (CRI) is a major complication in patients undergoing hemodialysis. The lack of high-throughput research on catheter-related microbiota makes it difficult to predict the occurrence of CRI. Thus, this study aimed to delineate the microbial structure and di...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | Renal Failure |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/0886022X.2023.2256421 |
| _version_ | 1797211827325632512 |
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| author | Ziyan Yan Yuchen Wang Wenli Zeng Renfei Xia Yanna Liu Zhouting Wu Wenfeng Deng Miao Zhu Jian Xu Haijun Deng Yun Miao |
| author_facet | Ziyan Yan Yuchen Wang Wenli Zeng Renfei Xia Yanna Liu Zhouting Wu Wenfeng Deng Miao Zhu Jian Xu Haijun Deng Yun Miao |
| author_sort | Ziyan Yan |
| collection | DOAJ |
| description | AbstractBackground Catheter-related infection (CRI) is a major complication in patients undergoing hemodialysis. The lack of high-throughput research on catheter-related microbiota makes it difficult to predict the occurrence of CRI. Thus, this study aimed to delineate the microbial structure and diversity landscape of hemodialysis catheter tips among patients during the perioperative period of kidney transplantation (KTx) and provide insights into predicting the occurrence of CRI.Methods Forty patients at the Department of Transplantation undergoing hemodialysis catheter removal were prospectively included. Samples, including catheter tip, catheter outlet skin swab, catheter blood, peripheral blood, oropharynx swab, and midstream urine, from the separate pre- and post-KTx groups were collected and analyzed using metagenomic next-generation sequencing (mNGS). All the catheter tips and blood samples were cultured conventionally.Results The positive detection rates for bacteria using mNGS and traditional culture were 97.09% (200/206) and 2.65% (3/113), respectively. Low antibiotic-sensitivity biofilms with colonized bacteria were detected at the catheter tip. In asymptomatic patients, no statistically significant difference was observed in the catheter tip microbial composition and diversity between the pre- and post-KTx group. The catheter tip microbial composition and diversity were associated with fasting blood glucose levels. Microorganisms at the catheter tip most likely originated from catheter outlet skin and peripheral blood.Conclusions The long-term colonization microbiota at the catheter tip is in a relatively stable state and is not readily influenced by KTx. It does not act as the source of infection in all CRIs, but could reflect hematogenous infection to some extent. |
| first_indexed | 2024-03-11T23:45:13Z |
| format | Article |
| id | doaj.art-a68ef678a0e14c69be5944eec6fb2f7e |
| institution | Directory Open Access Journal |
| issn | 0886-022X 1525-6049 |
| language | English |
| last_indexed | 2024-04-24T10:32:40Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Renal Failure |
| spelling | doaj.art-a68ef678a0e14c69be5944eec6fb2f7e2024-04-12T14:34:28ZengTaylor & Francis GroupRenal Failure0886-022X1525-60492023-12-0145210.1080/0886022X.2023.2256421Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysisZiyan Yan0Yuchen Wang1Wenli Zeng2Renfei Xia3Yanna Liu4Zhouting Wu5Wenfeng Deng6Miao Zhu7Jian Xu8Haijun Deng9Yun Miao10Department of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, Guangzhou, P.R. ChinaDepartment of Transplantation, Nanfang Hospital, Southern Medical Univerisity, Guangzhou, P.R. ChinaDepartment of Transplantation, Nanfang Hospital, Southern Medical Univerisity, Guangzhou, P.R. ChinaDepartment of Transplantation, Nanfang Hospital, Southern Medical Univerisity, Guangzhou, P.R. ChinaDepartment of Microbiology and Infectious Disease Center, School of Basic Medical Sciences, Peking University Health Science Center, Beijing, P.R. ChinaDepartment of Transplantation, Nanfang Hospital, Southern Medical Univerisity, Guangzhou, P.R. ChinaDepartment of Transplantation, Nanfang Hospital, Southern Medical Univerisity, Guangzhou, P.R. ChinaDepartment of Bioinformatics and System Development, Dinfectome Inc, Nanjing, P.R. ChinaDepartment of Transplantation, Nanfang Hospital, Southern Medical Univerisity, Guangzhou, P.R. ChinaDepartment of General Surgery & Guangdong Provincial Key Laboratory of Precision Medicine for Gastrointestinal Tumor, Nanfang Hospital, The First School of Clinical Medicine, Southern Medical University, Guangzhou, P.R. ChinaDepartment of Transplantation, Nanfang Hospital, Southern Medical Univerisity, Guangzhou, P.R. ChinaAbstractBackground Catheter-related infection (CRI) is a major complication in patients undergoing hemodialysis. The lack of high-throughput research on catheter-related microbiota makes it difficult to predict the occurrence of CRI. Thus, this study aimed to delineate the microbial structure and diversity landscape of hemodialysis catheter tips among patients during the perioperative period of kidney transplantation (KTx) and provide insights into predicting the occurrence of CRI.Methods Forty patients at the Department of Transplantation undergoing hemodialysis catheter removal were prospectively included. Samples, including catheter tip, catheter outlet skin swab, catheter blood, peripheral blood, oropharynx swab, and midstream urine, from the separate pre- and post-KTx groups were collected and analyzed using metagenomic next-generation sequencing (mNGS). All the catheter tips and blood samples were cultured conventionally.Results The positive detection rates for bacteria using mNGS and traditional culture were 97.09% (200/206) and 2.65% (3/113), respectively. Low antibiotic-sensitivity biofilms with colonized bacteria were detected at the catheter tip. In asymptomatic patients, no statistically significant difference was observed in the catheter tip microbial composition and diversity between the pre- and post-KTx group. The catheter tip microbial composition and diversity were associated with fasting blood glucose levels. Microorganisms at the catheter tip most likely originated from catheter outlet skin and peripheral blood.Conclusions The long-term colonization microbiota at the catheter tip is in a relatively stable state and is not readily influenced by KTx. It does not act as the source of infection in all CRIs, but could reflect hematogenous infection to some extent.https://www.tandfonline.com/doi/10.1080/0886022X.2023.2256421Hemodialysis catheter-related infectioncatheter tipperioperative period of kidney transplantationbiofilmmetagenome next-generation sequencing |
| spellingShingle | Ziyan Yan Yuchen Wang Wenli Zeng Renfei Xia Yanna Liu Zhouting Wu Wenfeng Deng Miao Zhu Jian Xu Haijun Deng Yun Miao Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysis Renal Failure Hemodialysis catheter-related infection catheter tip perioperative period of kidney transplantation biofilm metagenome next-generation sequencing |
| title | Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysis |
| title_full | Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysis |
| title_fullStr | Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysis |
| title_full_unstemmed | Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysis |
| title_short | Microbiota of long-term indwelling hemodialysis catheters during renal transplantation perioperative period: a cross-sectional metagenomic microbial community analysis |
| title_sort | microbiota of long term indwelling hemodialysis catheters during renal transplantation perioperative period a cross sectional metagenomic microbial community analysis |
| topic | Hemodialysis catheter-related infection catheter tip perioperative period of kidney transplantation biofilm metagenome next-generation sequencing |
| url | https://www.tandfonline.com/doi/10.1080/0886022X.2023.2256421 |
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