The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> Infection
<i>Lactobacillus fermentum</i> UCO-979C (Lf979C) beneficially modulates the cytokine response of gastric epithelial cells and macrophages after <i>Helicobacter pylori</i> infection in vitro. Nevertheless, no in vivo studies were performed with this strain to confirm its benef...
| প্রধান লেখক: | , , , , , , , , |
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| বিন্যাস: | প্রবন্ধ |
| ভাষা: | English |
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MDPI AG
2020-03-01
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| মালা: | Microorganisms |
| বিষয়গুলি: | |
| অনলাইন ব্যবহার করুন: | https://www.mdpi.com/2076-2607/8/4/479 |
| _version_ | 1827761378708946944 |
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| author | Valeria Garcia-Castillo Guillermo Marcial Leonardo Albarracín Mikado Tomokiyo Patricia Clua Hideki Takahashi Haruki Kitazawa Apolinaria Garcia-Cancino Julio Villena |
| author_facet | Valeria Garcia-Castillo Guillermo Marcial Leonardo Albarracín Mikado Tomokiyo Patricia Clua Hideki Takahashi Haruki Kitazawa Apolinaria Garcia-Cancino Julio Villena |
| author_sort | Valeria Garcia-Castillo |
| collection | DOAJ |
| description | <i>Lactobacillus fermentum</i> UCO-979C (Lf979C) beneficially modulates the cytokine response of gastric epithelial cells and macrophages after <i>Helicobacter pylori</i> infection in vitro. Nevertheless, no in vivo studies were performed with this strain to confirm its beneficial immunomodulatory effects. This work evaluated whether Lf979C improves protection against <i>H. pylori</i> infection in mice by modulating the innate immune response. In addition, we evaluated whether its exopolysaccharide (EPS) was involved in its beneficial effects. Lf979C significantly reduced TNF-α, IL-8, and MCP-1 and augmented IFN-γ and IL-10 in the gastric mucosa of <i>H. pylori</i>-infected mice. The differential cytokine profile induced by Lf979C in <i>H. pylori</i>-infected mice correlated with an improved reduction in the pathogen gastric colonization and protection against inflammatory damage. The purified EPS of Lf979C reduced IL-8 and enhanced IL-10 levels in the gastric mucosa of infected mice, while no effect was observed for IFN-γ. This work demonstrates for the first time the in vivo ability of Lf979C to increase resistance against <i>H. pylori</i> infection by modulating the gastric innate immune response. In addition, we advanced knowledge of the mechanisms involved in the beneficial effects of Lf979C by demonstrating that its EPS is partially responsible for its immunomodulatory effect. |
| first_indexed | 2024-03-11T10:12:02Z |
| format | Article |
| id | doaj.art-a690fb56e088452aa57276c124d3ab8c |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T10:12:02Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a690fb56e088452aa57276c124d3ab8c2023-11-16T14:29:23ZengMDPI AGMicroorganisms2076-26072020-03-018447910.3390/microorganisms8040479The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> InfectionValeria Garcia-Castillo0Guillermo Marcial1Leonardo Albarracín2Mikado Tomokiyo3Patricia Clua4Hideki Takahashi5Haruki Kitazawa6Apolinaria Garcia-Cancino7Julio Villena8Laboratory of Bacterial Pathogenicity, Faculty of Biological Sciences, University of Concepcion, Concepcion Bio Bio 4030000, ChileLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman CP4000, ArgentinaLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman CP4000, ArgentinaFood and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman CP4000, ArgentinaLaboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanFood and Feed Immunology Group, Graduate School of Agricultural Science, Tohoku University, Sendai 981-8555, JapanLaboratory of Bacterial Pathogenicity, Faculty of Biological Sciences, University of Concepcion, Concepcion Bio Bio 4030000, ChileLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli (CERELA-CONICET), Tucuman CP4000, Argentina<i>Lactobacillus fermentum</i> UCO-979C (Lf979C) beneficially modulates the cytokine response of gastric epithelial cells and macrophages after <i>Helicobacter pylori</i> infection in vitro. Nevertheless, no in vivo studies were performed with this strain to confirm its beneficial immunomodulatory effects. This work evaluated whether Lf979C improves protection against <i>H. pylori</i> infection in mice by modulating the innate immune response. In addition, we evaluated whether its exopolysaccharide (EPS) was involved in its beneficial effects. Lf979C significantly reduced TNF-α, IL-8, and MCP-1 and augmented IFN-γ and IL-10 in the gastric mucosa of <i>H. pylori</i>-infected mice. The differential cytokine profile induced by Lf979C in <i>H. pylori</i>-infected mice correlated with an improved reduction in the pathogen gastric colonization and protection against inflammatory damage. The purified EPS of Lf979C reduced IL-8 and enhanced IL-10 levels in the gastric mucosa of infected mice, while no effect was observed for IFN-γ. This work demonstrates for the first time the in vivo ability of Lf979C to increase resistance against <i>H. pylori</i> infection by modulating the gastric innate immune response. In addition, we advanced knowledge of the mechanisms involved in the beneficial effects of Lf979C by demonstrating that its EPS is partially responsible for its immunomodulatory effect.https://www.mdpi.com/2076-2607/8/4/479<i>Lactobacillus fermentum</i> UCO-979Cexopolysaccharide<i>H. pylori</i>gastric cytokinesimmunobiotics |
| spellingShingle | Valeria Garcia-Castillo Guillermo Marcial Leonardo Albarracín Mikado Tomokiyo Patricia Clua Hideki Takahashi Haruki Kitazawa Apolinaria Garcia-Cancino Julio Villena The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> Infection Microorganisms <i>Lactobacillus fermentum</i> UCO-979C exopolysaccharide <i>H. pylori</i> gastric cytokines immunobiotics |
| title | The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> Infection |
| title_full | The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> Infection |
| title_fullStr | The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> Infection |
| title_full_unstemmed | The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> Infection |
| title_short | The Exopolysaccharide of <i>Lactobacillus fermentum</i> UCO-979C Is Partially Involved in Its Immunomodulatory Effect and Its Ability to Improve the Resistance against <i>Helicobacter pylori</i> Infection |
| title_sort | exopolysaccharide of i lactobacillus fermentum i uco 979c is partially involved in its immunomodulatory effect and its ability to improve the resistance against i helicobacter pylori i infection |
| topic | <i>Lactobacillus fermentum</i> UCO-979C exopolysaccharide <i>H. pylori</i> gastric cytokines immunobiotics |
| url | https://www.mdpi.com/2076-2607/8/4/479 |
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