Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stability
Abstract β-Escin is a mixture of triterpenoid saponins extracted from horse chestnut seeds that have diverse pharmacological activities, including anti-inflammation, anti-edematous, venotonic, and antiviral effects. In the clinical setting, β-escin is primarily used to treat venous insufficiency and...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36871-1 |
| _version_ | 1827916978567774208 |
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| author | Zheng-Zong Lai Hsin-Hsuen Shen Yen-Mei Lee |
| author_facet | Zheng-Zong Lai Hsin-Hsuen Shen Yen-Mei Lee |
| author_sort | Zheng-Zong Lai |
| collection | DOAJ |
| description | Abstract β-Escin is a mixture of triterpenoid saponins extracted from horse chestnut seeds that have diverse pharmacological activities, including anti-inflammation, anti-edematous, venotonic, and antiviral effects. In the clinical setting, β-escin is primarily used to treat venous insufficiency and blunt trauma injuries. The anti-Zika virus (ZIKV) activity of β-escin has not been explored. This study investigated the antiviral efficacy of β-escin on ZIKV and dengue virus (DENV) in vitro and then elucidated the underlying mechanism. The inhibitory effects of β-escin on viral RNA synthesis, protein levels, and infection ability were determined using qRT-PCR, Western blotting, and immunofluorescence assays, respectively. To further characterize how β-escin interferes with the viral life cycle, the time-of-addition experiment was performed. An inactivation assay was performed to determine whether β-escin affects ZIKV virion stability. To broaden these findings, the antiviral effects of β-escin on different DENV serotypes were assessed using dose-inhibition and time-of-addition assays. The results showed that β-escin exhibits anti-ZIKV activity by decreasing viral RNA levels, protein expression, progeny yield, and virion stability. β-Escin inhibited ZIKV infection by disrupting viral binding and replication. Furthermore, β-escin demonstrated antiviral activities against four DENV serotypes in a Vero cell model and prophylactic protection against ZIKV and DENV infections. |
| first_indexed | 2024-03-13T03:22:27Z |
| format | Article |
| id | doaj.art-a69878416e1f4f05a458be7a94a6e399 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T03:22:27Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a69878416e1f4f05a458be7a94a6e3992023-06-25T11:17:13ZengNature PortfolioScientific Reports2045-23222023-06-0113111010.1038/s41598-023-36871-1Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stabilityZheng-Zong Lai0Hsin-Hsuen Shen1Yen-Mei Lee2Graduate Institute of Medical Science, National Defense Medical CenterDepartment and Graduate Institute of Pharmacology, National Defense Medical CenterDepartment and Graduate Institute of Pharmacology, National Defense Medical CenterAbstract β-Escin is a mixture of triterpenoid saponins extracted from horse chestnut seeds that have diverse pharmacological activities, including anti-inflammation, anti-edematous, venotonic, and antiviral effects. In the clinical setting, β-escin is primarily used to treat venous insufficiency and blunt trauma injuries. The anti-Zika virus (ZIKV) activity of β-escin has not been explored. This study investigated the antiviral efficacy of β-escin on ZIKV and dengue virus (DENV) in vitro and then elucidated the underlying mechanism. The inhibitory effects of β-escin on viral RNA synthesis, protein levels, and infection ability were determined using qRT-PCR, Western blotting, and immunofluorescence assays, respectively. To further characterize how β-escin interferes with the viral life cycle, the time-of-addition experiment was performed. An inactivation assay was performed to determine whether β-escin affects ZIKV virion stability. To broaden these findings, the antiviral effects of β-escin on different DENV serotypes were assessed using dose-inhibition and time-of-addition assays. The results showed that β-escin exhibits anti-ZIKV activity by decreasing viral RNA levels, protein expression, progeny yield, and virion stability. β-Escin inhibited ZIKV infection by disrupting viral binding and replication. Furthermore, β-escin demonstrated antiviral activities against four DENV serotypes in a Vero cell model and prophylactic protection against ZIKV and DENV infections.https://doi.org/10.1038/s41598-023-36871-1 |
| spellingShingle | Zheng-Zong Lai Hsin-Hsuen Shen Yen-Mei Lee Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stability Scientific Reports |
| title | Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stability |
| title_full | Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stability |
| title_fullStr | Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stability |
| title_full_unstemmed | Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stability |
| title_short | Inhibitory effect of β-escin on Zika virus infection through the interruption of viral binding, replication, and stability |
| title_sort | inhibitory effect of β escin on zika virus infection through the interruption of viral binding replication and stability |
| url | https://doi.org/10.1038/s41598-023-36871-1 |
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