Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer
Abstract Background Blood-based tests have public appeal in screening cancers due to their minimally invasive nature, ability to integrate with other routine blood tests, and high compliance. This study aimed to investigate whether certain epigenetic modulation of peripheral blood mononuclear cells...
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Epigenetics & Chromatin |
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| Online Access: | https://doi.org/10.1186/s13072-023-00509-3 |
| _version_ | 1827633071492431872 |
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| author | Jia-Yi Hou Ning Li Jie Wang Li-Juan Gao Jia-Song Chang Ji-Min Cao |
| author_facet | Jia-Yi Hou Ning Li Jie Wang Li-Juan Gao Jia-Song Chang Ji-Min Cao |
| author_sort | Jia-Yi Hou |
| collection | DOAJ |
| description | Abstract Background Blood-based tests have public appeal in screening cancers due to their minimally invasive nature, ability to integrate with other routine blood tests, and high compliance. This study aimed to investigate whether certain epigenetic modulation of peripheral blood mononuclear cells (PBMCs) could be a biomarker of colorectal cancer (CRC). Results Western blotting of histones in the PBMCs from 40 colorectal cancer patients and 40 healthy controls was performed to identify the crotonylation sites of proteins. The correlation of crotonylation with tumor staging and diagnostic efficacy were analyzed. Crotonylation of H2BK12 (H2BK12cr) was identified significantly upregulated in the PBMCs of CRC patients compared to healthy controls, and were closely related to distant metastasis (P = 0.0478) and late TNM stage (P = 0.0201). Receiver operator characteristic curve (ROC) analysis demonstrated that the area under curve (AUC) of H2BK12cr was 0.8488, the sensitivity was 70%, and the specificity was 92.5%. The H2BK12cr parameter significantly increased the diagnostic effectiveness of CRC compared with the commercial carcinoembryonic antigen assays. Conclusions The H2BK12cr level in PBMCs of CRC patients has a potential to be a biomarker for distinguishing CRC patients from healthy controls with the advantages of easy operation and high diagnostic efficacy. |
| first_indexed | 2024-03-09T14:54:26Z |
| format | Article |
| id | doaj.art-a698e1cc1bf34f90b06cd422e66ff350 |
| institution | Directory Open Access Journal |
| issn | 1756-8935 |
| language | English |
| last_indexed | 2024-03-09T14:54:26Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Epigenetics & Chromatin |
| spelling | doaj.art-a698e1cc1bf34f90b06cd422e66ff3502023-11-26T14:16:25ZengBMCEpigenetics & Chromatin1756-89352023-09-0116111010.1186/s13072-023-00509-3Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancerJia-Yi Hou0Ning Li1Jie Wang2Li-Juan Gao3Jia-Song Chang4Ji-Min Cao5Department of Clinical Laboratory, Shanxi Provincial Academy of Traditional Chinese MedicineDepartment of Gastrointestinal and Pancreatic Surgery and Hernia and Abdominal Surgery, Shanxi Provincial People’s HospitalKey Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, and the Department of Physiology, Shanxi Medical UniversityKey Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, and the Department of Physiology, Shanxi Medical UniversityKey Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, and the Department of Physiology, Shanxi Medical UniversityKey Laboratory of Cellular Physiology at Shanxi Medical University, Ministry of Education, Key Laboratory of Cellular Physiology of Shanxi Province, and the Department of Physiology, Shanxi Medical UniversityAbstract Background Blood-based tests have public appeal in screening cancers due to their minimally invasive nature, ability to integrate with other routine blood tests, and high compliance. This study aimed to investigate whether certain epigenetic modulation of peripheral blood mononuclear cells (PBMCs) could be a biomarker of colorectal cancer (CRC). Results Western blotting of histones in the PBMCs from 40 colorectal cancer patients and 40 healthy controls was performed to identify the crotonylation sites of proteins. The correlation of crotonylation with tumor staging and diagnostic efficacy were analyzed. Crotonylation of H2BK12 (H2BK12cr) was identified significantly upregulated in the PBMCs of CRC patients compared to healthy controls, and were closely related to distant metastasis (P = 0.0478) and late TNM stage (P = 0.0201). Receiver operator characteristic curve (ROC) analysis demonstrated that the area under curve (AUC) of H2BK12cr was 0.8488, the sensitivity was 70%, and the specificity was 92.5%. The H2BK12cr parameter significantly increased the diagnostic effectiveness of CRC compared with the commercial carcinoembryonic antigen assays. Conclusions The H2BK12cr level in PBMCs of CRC patients has a potential to be a biomarker for distinguishing CRC patients from healthy controls with the advantages of easy operation and high diagnostic efficacy.https://doi.org/10.1186/s13072-023-00509-3Colorectal cancerBiomarkerCrotonylationH2BK12Peripheral blood mononuclear cells |
| spellingShingle | Jia-Yi Hou Ning Li Jie Wang Li-Juan Gao Jia-Song Chang Ji-Min Cao Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer Epigenetics & Chromatin Colorectal cancer Biomarker Crotonylation H2BK12 Peripheral blood mononuclear cells |
| title | Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer |
| title_full | Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer |
| title_fullStr | Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer |
| title_full_unstemmed | Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer |
| title_short | Histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer |
| title_sort | histone crotonylation of peripheral blood mononuclear cells is a potential biomarker for diagnosis of colorectal cancer |
| topic | Colorectal cancer Biomarker Crotonylation H2BK12 Peripheral blood mononuclear cells |
| url | https://doi.org/10.1186/s13072-023-00509-3 |
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