Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium
Atherosclerosis is a complex non-monogenic disease related to endothelial damage in elastic-type arteries and incorrect feeding. Here, using cryodamage of endothelial cells (ECs) of rat abdominal aorta, we examined the role of the EC basement membrane (BM) for re-endothelization endothelial regenera...
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MDPI AG
2022-11-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/11/2858 |
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| author | Irina S. Sesorova Vitaly V. Sesorov Pavel B. Soloviev Konstantin Yu. Lakunin Ivan D. Dimov Alexander A. Mironov |
| author_facet | Irina S. Sesorova Vitaly V. Sesorov Pavel B. Soloviev Konstantin Yu. Lakunin Ivan D. Dimov Alexander A. Mironov |
| author_sort | Irina S. Sesorova |
| collection | DOAJ |
| description | Atherosclerosis is a complex non-monogenic disease related to endothelial damage in elastic-type arteries and incorrect feeding. Here, using cryodamage of endothelial cells (ECs) of rat abdominal aorta, we examined the role of the EC basement membrane (BM) for re-endothelization endothelial regeneration and its ability to capture low density lipoproteins (LDLs). Regeneration of endothelium induced thickening of the ECBM. Secretion of the BM components occurred in the G2-phase. Multiple regenerations, as well as arterial hypertension and aging, also led to the thickening of the BM. Under these conditions, the speed of re-endothelialization increased. The thick BM captured more LDLs. LDLs formed after overloading of rats with lipids acquired higher affinity to the BM, presumably due to the prolonged transport of chylomicrons through neuraminidase-positive endo-lysosomes. These data provide new molecular and cellular mechanisms of atherogenesis. |
| first_indexed | 2024-03-09T19:15:24Z |
| format | Article |
| id | doaj.art-a69df7b90b6b43229459d40ccd89c187 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T19:15:24Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj.art-a69df7b90b6b43229459d40ccd89c1872023-11-24T03:51:52ZengMDPI AGBiomedicines2227-90592022-11-011011285810.3390/biomedicines10112858Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic EndotheliumIrina S. Sesorova0Vitaly V. Sesorov1Pavel B. Soloviev2Konstantin Yu. Lakunin3Ivan D. Dimov4Alexander A. Mironov5Department of Anatomy, Ivanovo State Medical Academy, 153012 Ivanovo, RussiaDepartment of Anatomy, Ivanovo State Medical Academy, 153012 Ivanovo, RussiaDepartment of Pathological Anatomy, Ivanovo State Medical Academy, 153012 Ivanovo, RussiaCentral District Hospital of Noginsk, 142400 Noginsk, RussiaDepartment of Anatomy, Saint Petersburg State Pediatric Medical University, 194100 Saint Petersburg, RussiaItalian Foundation for Cancer Research Institute of Molecular Oncology, Via Adamello 16, 20139 Milan, ItalyAtherosclerosis is a complex non-monogenic disease related to endothelial damage in elastic-type arteries and incorrect feeding. Here, using cryodamage of endothelial cells (ECs) of rat abdominal aorta, we examined the role of the EC basement membrane (BM) for re-endothelization endothelial regeneration and its ability to capture low density lipoproteins (LDLs). Regeneration of endothelium induced thickening of the ECBM. Secretion of the BM components occurred in the G2-phase. Multiple regenerations, as well as arterial hypertension and aging, also led to the thickening of the BM. Under these conditions, the speed of re-endothelialization increased. The thick BM captured more LDLs. LDLs formed after overloading of rats with lipids acquired higher affinity to the BM, presumably due to the prolonged transport of chylomicrons through neuraminidase-positive endo-lysosomes. These data provide new molecular and cellular mechanisms of atherogenesis.https://www.mdpi.com/2227-9059/10/11/2858endothelial cellatherosclerosisregenerationLDLenterocyteslipid overloading |
| spellingShingle | Irina S. Sesorova Vitaly V. Sesorov Pavel B. Soloviev Konstantin Yu. Lakunin Ivan D. Dimov Alexander A. Mironov Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium Biomedicines endothelial cell atherosclerosis regeneration LDL enterocytes lipid overloading |
| title | Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium |
| title_full | Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium |
| title_fullStr | Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium |
| title_full_unstemmed | Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium |
| title_short | Role of Endothelial Regeneration and Overloading of Enterocytes with Lipids in Capturing of Lipoproteins by Basement Membrane of Rat Aortic Endothelium |
| title_sort | role of endothelial regeneration and overloading of enterocytes with lipids in capturing of lipoproteins by basement membrane of rat aortic endothelium |
| topic | endothelial cell atherosclerosis regeneration LDL enterocytes lipid overloading |
| url | https://www.mdpi.com/2227-9059/10/11/2858 |
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